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Legendplex multiplex assay kit

Manufactured by BioLegend
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The LEGENDplex™ multiplex assay kit is a flow cytometry-based platform that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The kit provides a reliable and efficient method for analyzing complex biological samples, such as cell culture supernatants, serum, or plasma.

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Lab products found in correlation

Anti cd3 cd28 dynabeads, by Thermo Fisher Scientific (1 mentions) Synergy neo2 luminescence microplate reader, by Agilent Technologies (1 mentions) Griess reagent, by Promega (1 mentions) Cytokine bead array mouse inflammation kit, by BD (1 mentions)

Close spelling variants of this product

LEGENDplex (292 citations) LEGENDplex™ kit (64 citations) LEGENDplex assay (51 citations) LEGENDplex multiplex assay (12 citations) Multiplex assay (3 citations)

3 protocols using legendplex multiplex assay kit

1

Quantifying Macrophage Cytokine Secretion

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After 24 h of incubation, the supernatant of the macrophages grown on the bare and coated 316 L SS samples were collected, 6 inflammatory cytokines secreted by macrophages were detected by using BioLegend's LEGENDplex™ multiplex assay kit based on flow cytometry, including 3 pro-inflammatory cytokines (IL-6, TNF-α, IL-1β)and 3 anti-inflammatory cytokines (TGF-β1, IL-23, and IL-10).
Tan X., Gao P., Li Y., Qi P., Liu J., Shen R., Wang L., Huang N., Xiong K., Tian W, & Tu Q. (2020). Poly-dopamine, poly-levodopa, and poly-norepinephrine coatings: Comparison of physico-chemical and biological properties with focus on the application for blood-contacting devices. Bioactive Materials, 6(1), 285-296.
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2

Cytotoxicity Assay for CAR-T Cells

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Control PBMC T cells and iPSC-derived T cells were activated (day 0) using anti-CD3/CD28 Dynabeads (Gibco, 11131D) or CD3/CD28 T activator (StemCell Tech, 10971), followed by transduction with a lentiviral vector encoding the CD19-CAR 24-hours later (Scarfò et al., 2018 (link)). T cells were cultured in RPMI media containing 10% fetal bovine serum, penicillin, streptomycin and supplemented with 20 IU/ml rhIL-2 beginning on day 0 of culture and were maintained at a constant cell concentration of 0.5 x106/mL by counting every 2-3 days. On day 10 cells were de-beaded (when using Dynabeads) and used for assays. For cytotoxicity assays, control PBMC or iPSC-T cells were co-cultured with luciferase-expressing Jeko-1 or OCI-Ly1 tumor cells at the indicated ratios for 18 hours. Luciferase activity was measured with a Synergy Neo2 luminescence microplate reader (Biotek). Percentage of specific lysis was calculated as (total RLU / target cells only RLU) x100. Cell-free supernatants were collected for cytokine release assay. Levels of cytokines were measured using a LEGENDplex Multiplex Assay Kit (Biolegend, 741030) following manufacture's instructions.
Jing R., Scarfo I., Najia M., da Rocha E.L., Han A., Sanborn M., Bingham T., Kubaczka C., Jha D., Falchetti M., Schlaeger T.M., North T.E., Maus M.V, & Daley G.Q. (2022). EZH1 repression generates mature iPSC-derived CAR T cells with enhanced antitumor activity. Cell stem cell, 29(8), 1181-1196.e6.
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3

Cytokine and Nitric Oxide Production in MRSA-Infected Macrophages

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The protein levels of IL-12p70, TNF-α, IFN-γ, MCP-1 and IL-6 were determined using the Cytokine Bead Array Mouse Inflammation Kit (BD Bioscience) and LEGENDplex Multiplex Assay kit (BioLegend, San Diego, CA, USA) following the manufacturer's instructions. IL-10 levels were measured by the Cytokine Beads Array Mouse IL-10 Enhanced Sensitivity Flex Set (BD Bioscience). The NO assay was performed as described (29 (link)). Briefly, macrophages were treated with 5 MOI of MRSA for 8 h, and the culture medium was collected to measure the amount of nitrite, a stable metabolite of NO. One hundred microliters of the culture medium were incubated with equal amount of Griess reagent (Promega, Madison, WI, USA) at room temperature for 10 min, and the absorbance was measured at 540 nm using a microplate reader. The quantity of nitrite was determined from a standard curve of sodium nitrate.
Sim H., Jeong D., Kim H.I., Pak S., Thapa B., Kwon H.J, & Lee K. (2021). CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages. Immune Network, 21(2), e13.
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