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Recombinant human ifn λ2

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Recombinant human IFN-λ2 is a cytokine protein produced using recombinant DNA technology. It is a type III interferon that functions in the innate immune response.

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Lab products found in correlation

Recombinant human ifn β, by Thermo Fisher Scientific (2 mentions) Lactacystin, by Merck Group (1 mentions) Poly 1 c lmw, by InvivoGen (1 mentions) Recombinant mouse ifn β, by PBL Assay Science (1 mentions) Cd127 a7r34, by BioLegend (1 mentions) Ifn β, by Thermo Fisher Scientific (1 mentions) Recombinant mouse ifn λ2, by Thermo Fisher Scientific (1 mentions) Pyridone 6, by Abcam (1 mentions) Ag490, by Bio-Techne (1 mentions) Fludarabine, by Bio-Techne (1 mentions) Recombinant mouse tnf, by Thermo Fisher Scientific (1 mentions) Puromycin, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Recombinant human ifn γ, by BioLegend (1 mentions) Recombinant human ifn α2, by BioLegend (1 mentions)

Close spelling variants of this product

Recombinant murine and human IFN-λ2 and IL-22 IFN-λ2

3 protocols using recombinant human ifn λ2

1

Modulation of Cellular Signaling Pathways

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Cells were treated by ALLN (Calbiochem, 208719) at 25μM during 5hrs, Lactacystin (Sigma, L-6785) at 10μM during 5hrs, recombinant human IFN-β (Peprotech, 300-02BC) at 1ng/mL during 24hrs, recombinant human IFN-λ2 (Peprotech, 300-02K) at 2ng/mL during 24hrs, Poly(I:C) LMW (InvivoGen) at 10μg/mL during 24hrs, or with 20% of culture medium conditioned by NHDFs during the exponential growth phase or at the senescence plateau during 48hrs.
Mound A., Goormachtigh G., Bray F., Flament S., Rolando C., Ruez R., Martin N., Decourcelle A., Dehennaut V., Saliou J.M., Chamaillard M, & Abbadie C. (2023). The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein. PLOS ONE, 18(1), e0279028.
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2

Immune Cell Profiling by Flow Cytometry

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For flow cytometry and sorting experiments, antibodies to CD4 (RM4-4), NK1.1 (PK136), CD49b (DX5), CD3 (17A2), Ly6G (1A8), B220 (RA3-6B2), CD11c (N418), CD45.2 (104), CD45.1 (A20), CX3CR1 (SA011F11), IAb (AF6-120.1), CD11b (Mac1), CD90 (G7), lineage markers (17A2/RB6-8C5/RA3-6B2/Ter-119/M1/70), and CD127 (A7R34) were purchased from BioLegend. To stimulate neutrophils in vitro and for in vivo administration, we used either recombinant mouse IFN-λ2 (purchased from Peprotech) or recombinant mouse IFN-λ2 to which polyethylene glycol was attached (provided by Bristol-Myers Squibb). Recombinant mouse IFN-β was purchased from PBL interferonsource. Recombinant human IFN-λ2 and IFN-β were purchased from Peprotech. Puromycin was purchased from Sigma. Where indicated, specific chemical inhibitors were used. To inhibit protein synthesis, cycloheximide (Sigma) was used at a concentration of 10 μg/ml. To inhibit Jak signaling, pyridone 6 (BioVision) was used. To inhibit STAT-1 signaling, fludarabine (Tocris) was used. To inhibit Jak2 signaling, either AG490 (Tocris) or 1,2,3,4,5,6-hexabromocyclohexane (HBC, Tocris) was used. To stimulate neutrophils, either E. coli LPS (Serotype O55:B5 TLR grade, purchased from Enzo) or recombinant mouse TNF (Peprotech) was used.
Broggi A., Tan Y., Granucci F, & Zanoni I. (2017). IFN-λ suppresses intestinal inflammation by non-translational regulation of neutrophil function. Nature immunology, 18(10), 1084-1093.
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3

Interferon-Induced Gene Expression Analysis

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HEK293, A549, and Calu-3 cells were incubated with different concentrations of recombinant human IFN-α2 (103, 104, and 105 U/mL; BioLegend), recombinant human IFN-β (103, 104, and 105 U/mL; PeproTech), recombinant human IFN-ω (105, 106, and 107 U/mL; PeproTech), recombinant human IFN-γ (103, 104, and 105 U/mL; BioLegend), and recombinant human IFN-λ2 (103, 104, and 105 U/mL; PeproTech), respectively. At 24 h poststimulation, the cells were harvested, and the gene expression levels of α-SNAP, ISG56, IP-10, and MX1 were determined by qPCR.
A549 cells were incubated with IFN-α2 (104 U/mL), IFN-β (104 U/mL), IFN-γ (104 U/mL), IFN-λ2 (104 U/mL), and IFN-ω (106 U/mL), respectively. At 12, 24, and 48 h poststimulation, the cells were harvested to determine α-SNAP mRNA levels by qPCR. The α-SNAP protein expression levels at 24 h poststimulation were determined by using flow cytometry.
Wang J., Luo J., Wen Z., Wang X., Shuai L., Zhong G., Wang C., Sun Z., Chen W., Ge J., Liu R., Wang X, & Bu Z. (2022). Alpha-Soluble NSF Attachment Protein Prevents the Cleavage of the SARS-CoV-2 Spike Protein by Functioning as an Interferon-Upregulated Furin Inhibitor. mBio, 13(1), e02443-21.
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