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Anti cd45ro

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Anti-CD45RO is a laboratory reagent used for the identification and characterization of CD45RO-positive cells in flow cytometric analyses. It is designed to bind to the CD45RO surface antigen, which is expressed on memory T cells. The reagent can be used to study the distribution and relative expression of CD45RO in various cell populations.

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Lab products found in correlation

Anti pd 1, by Merck Group (2 mentions) Anti ki67, by Agilent Technologies (2 mentions) Anti cd4, by StatLab (2 mentions) Anti cd8, by StatLab (2 mentions) Anti pd l1, by Thermo Fisher Scientific (2 mentions) Anti cd68, by Leica (2 mentions) Anti cd20, by Leica (2 mentions) Anti lag3, by Cell Signaling Technology (2 mentions) Cd244, by Proteintech (2 mentions) Cd160, by Abcam (2 mentions) Anti cd3 clone f7, by Agilent Technologies (1 mentions) Anti cd8 clone c8 144b, by Agilent Technologies (1 mentions)

Spelling variants of this product

Anti-human CD45RO conjugated to APC-efluor780 (2 citations) ) anti-human-CD45RO (UCHL1, (2 citations) Anti-CD45RO-PE-Cy7 (2 citations) Anti-CD45RO (UCHL1)( (2 citations)

3 protocols using anti cd45ro

1

Quantifying Tumor-Infiltrating Immune Cells

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All 68 frozen tumor specimens were analyzed for the presence of tumor infiltrating cells using immunohistochemistry with a panel of specific antibodies. Positive labeling was evaluated by two independent observers who did not know the clinico-pathological characteristics of the corresponding patients. The observers evaluated tissue tumor infiltrates with 10x and 40× objectives by analyzing tissue immunolabeling with the following antibodies: anti-CD45 (clone GRT2 produced in our laboratory) [43 (link)], anti-CD45RO (Thermo Fisher, Waltham, Massachusetts, USA), anti-CD8 (clone C8/144B DAKO, Glostrup, Denmark), and anti-CD3 (clone F7.2.38, DAKO, Glostrup, Denmark). Whole tumor sample infiltration was evaluated in 5 different fields under 40x objective and scored as: + (≤30 cells/field), ++ (30–70 cells/field), +++ (70–120 cells/field) and ++++ (≤120 cells/field). We determined an infiltration score (1–4) for each marker per tumor sample by assigning one point to each cross (+) for subsequent statistical analysis. In addition, for we counted the number of CD8 positive T cells per microscopic field at a 400x magnification. We calculated the average value of the five representative fields examined for each slide.
Perea F., Sánchez-Palencia A., Gómez-Morales M., Bernal M., Concha Á., García M.M., González-Ramírez A.R., Kerick M., Martin J., Garrido F., Ruiz-Cabello F, & Aptsiauri N. (2017). HLA class I loss and PD-L1 expression in lung cancer: impact on T-cell infiltration and immune escape. Oncotarget, 9(3), 4120-4133.
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2

Immunohistochemical Analysis of Tumor Samples

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Archival tissue from the autopsy was obtained and IHC evaluation was performed. Antibodies utilized were anti-CD45RO (Thermo Scientific, cat# MA5–11532, 1:1,600), anti-GZMB (Granzyme B, Biocare cat# ACI 3202 AA, prediluted), anti-Ki67 (Dako, cat# M7240, 1:200), anti-CD4 (StatLab, cat# RM27–10, prediluted), anti-CD8 (StatLab, cat# MM39–10, prediluted), anti-PD-L1 (Cat.#PA5–28115 ThermoFisher, Grand Island, NY; 1:7500), anti-CD68 (PA0191, Leica, Buffalo Grove, IL; prediluted), anti-PD-1 (HPA035981, Sigma-Aldrich Co., St. Louis, MO; 1:75), anti-CD20 (PA0906, Leica, Buffalo Grove, IL, prediluted), anti-LAG3 (Cell Signaling, Catalog# 15372, dilution 1:200), CD244 (Proteintech, Catalog# 16677–1-AP, dilution 1:200), CD160 (Abcam, Catalog# ab202845, dilution 1:600). The Bond Polymer Refine detection system or Envision system was used for visualization. Slides were then dehydrated, cleared, and coverslipped.
Johnson D.B., McDonnell W.J., Ericsson-Gonzalez P.I., Al-Rohil R., Mobley B.C., Salem J.E., Wang D.Y., Sanchez V., Wang Y., Chastain C.A., Barker K., Liang Y., Warren S., Beechem J., Menzies A.M., Tio M., Long G.V., Cohen J.V., Guidon A.C., O’Hare M., Chandra S., Chowdhary A., Lebrun-Vignes B., Goldinger S., Rushing E., Buchbinder E., Mallal S.A., Shi C., Xu Y., Moslehi J.J., Sanders M.E., Sosman J.A, & Balko J.M. (2019). A case report of clonal EBV-like memory CD4+ T cell activation in fatal checkpoint inhibitor-induced encephalitis. Nature medicine, 25(8), 1243-1250.
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3

Immunohistochemical Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Archival tissue from the autopsy was obtained and IHC evaluation was performed. Antibodies utilized were anti-CD45RO (Thermo Scientific, cat# MA5–11532, 1:1,600), anti-GZMB (Granzyme B, Biocare cat# ACI 3202 AA, prediluted), anti-Ki67 (Dako, cat# M7240, 1:200), anti-CD4 (StatLab, cat# RM27–10, prediluted), anti-CD8 (StatLab, cat# MM39–10, prediluted), anti-PD-L1 (Cat.#PA5–28115 ThermoFisher, Grand Island, NY; 1:7500), anti-CD68 (PA0191, Leica, Buffalo Grove, IL; prediluted), anti-PD-1 (HPA035981, Sigma-Aldrich Co., St. Louis, MO; 1:75), anti-CD20 (PA0906, Leica, Buffalo Grove, IL, prediluted), anti-LAG3 (Cell Signaling, Catalog# 15372, dilution 1:200), CD244 (Proteintech, Catalog# 16677–1-AP, dilution 1:200), CD160 (Abcam, Catalog# ab202845, dilution 1:600). The Bond Polymer Refine detection system or Envision system was used for visualization. Slides were then dehydrated, cleared, and coverslipped.
Johnson D.B., McDonnell W.J., Ericsson-Gonzalez P.I., Al-Rohil R., Mobley B.C., Salem J.E., Wang D.Y., Sanchez V., Wang Y., Chastain C.A., Barker K., Liang Y., Warren S., Beechem J., Menzies A.M., Tio M., Long G.V., Cohen J.V., Guidon A.C., O’Hare M., Chandra S., Chowdhary A., Lebrun-Vignes B., Goldinger S., Rushing E., Buchbinder E., Mallal S.A., Shi C., Xu Y., Moslehi J.J., Sanders M.E., Sosman J.A, & Balko J.M. (2019). A case report of clonal EBV-like memory CD4+ T cell activation in fatal checkpoint inhibitor-induced encephalitis. Nature medicine, 25(8), 1243-1250.
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