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Anti cd45ro

Manufactured by Thermo Fisher Scientific
Sourced in United States

Anti-CD45RO is a laboratory reagent used for the identification and characterization of CD45RO-positive cells in flow cytometric analyses. It is designed to bind to the CD45RO surface antigen, which is expressed on memory T cells. The reagent can be used to study the distribution and relative expression of CD45RO in various cell populations.

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3 protocols using anti cd45ro

1

Quantifying Tumor-Infiltrating Immune Cells

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All 68 frozen tumor specimens were analyzed for the presence of tumor infiltrating cells using immunohistochemistry with a panel of specific antibodies. Positive labeling was evaluated by two independent observers who did not know the clinico-pathological characteristics of the corresponding patients. The observers evaluated tissue tumor infiltrates with 10x and 40× objectives by analyzing tissue immunolabeling with the following antibodies: anti-CD45 (clone GRT2 produced in our laboratory) [43 (link)], anti-CD45RO (Thermo Fisher, Waltham, Massachusetts, USA), anti-CD8 (clone C8/144B DAKO, Glostrup, Denmark), and anti-CD3 (clone F7.2.38, DAKO, Glostrup, Denmark). Whole tumor sample infiltration was evaluated in 5 different fields under 40x objective and scored as: + (≤30 cells/field), ++ (30–70 cells/field), +++ (70–120 cells/field) and ++++ (≤120 cells/field). We determined an infiltration score (1–4) for each marker per tumor sample by assigning one point to each cross (+) for subsequent statistical analysis. In addition, for we counted the number of CD8 positive T cells per microscopic field at a 400x magnification. We calculated the average value of the five representative fields examined for each slide.
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2

Immunohistochemical Analysis of Tumor Samples

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Archival tissue from the autopsy was obtained and IHC evaluation was performed. Antibodies utilized were anti-CD45RO (Thermo Scientific, cat# MA5–11532, 1:1,600), anti-GZMB (Granzyme B, Biocare cat# ACI 3202 AA, prediluted), anti-Ki67 (Dako, cat# M7240, 1:200), anti-CD4 (StatLab, cat# RM27–10, prediluted), anti-CD8 (StatLab, cat# MM39–10, prediluted), anti-PD-L1 (Cat.#PA5–28115 ThermoFisher, Grand Island, NY; 1:7500), anti-CD68 (PA0191, Leica, Buffalo Grove, IL; prediluted), anti-PD-1 (HPA035981, Sigma-Aldrich Co., St. Louis, MO; 1:75), anti-CD20 (PA0906, Leica, Buffalo Grove, IL, prediluted), anti-LAG3 (Cell Signaling, Catalog# 15372, dilution 1:200), CD244 (Proteintech, Catalog# 16677–1-AP, dilution 1:200), CD160 (Abcam, Catalog# ab202845, dilution 1:600). The Bond Polymer Refine detection system or Envision system was used for visualization. Slides were then dehydrated, cleared, and coverslipped.
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3

Immunohistochemical Analysis of Tumor Samples

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Archival tissue from the autopsy was obtained and IHC evaluation was performed. Antibodies utilized were anti-CD45RO (Thermo Scientific, cat# MA5–11532, 1:1,600), anti-GZMB (Granzyme B, Biocare cat# ACI 3202 AA, prediluted), anti-Ki67 (Dako, cat# M7240, 1:200), anti-CD4 (StatLab, cat# RM27–10, prediluted), anti-CD8 (StatLab, cat# MM39–10, prediluted), anti-PD-L1 (Cat.#PA5–28115 ThermoFisher, Grand Island, NY; 1:7500), anti-CD68 (PA0191, Leica, Buffalo Grove, IL; prediluted), anti-PD-1 (HPA035981, Sigma-Aldrich Co., St. Louis, MO; 1:75), anti-CD20 (PA0906, Leica, Buffalo Grove, IL, prediluted), anti-LAG3 (Cell Signaling, Catalog# 15372, dilution 1:200), CD244 (Proteintech, Catalog# 16677–1-AP, dilution 1:200), CD160 (Abcam, Catalog# ab202845, dilution 1:600). The Bond Polymer Refine detection system or Envision system was used for visualization. Slides were then dehydrated, cleared, and coverslipped.
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