Cells were grown overnight in 24-well plates at 37°C in a 5% CO2 atmosphere. After reaching over 80% confluence, the plasmids mRuby-Clathrin (Addgene #55852), pEGFP-Sec23A (Addgene #66609), ZsGreen-Rab5 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.), EGFP-Rab7A (Addgene #28047), GFP-LAMP1 (Addgene #16290), EMTB-3XGFP (Addgene #26741), pSNAPf-Cox8A (Addgene #101129), or pSNAPf-TOMM20 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.) was transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. After 24 h, the transfected cells were digested with trypsin-EDTA and seeded into Nunc Glass Bottom Dishes (Φ 12 mm, Thermo Fisher Scientific, Inc.) at a density of 1.5 ~ 2.0 × 104 per well in growth medium (150 μL). The cells were grown for an additional 12 ~ 24 h before incubation with the indicated probes.
Psnapf tomm20
Manufactured by Thermo Fisher Scientific
Sourced in China
The PSNAPf-TOMM20 is a laboratory instrument used for the analysis of protein interactions. It is designed to detect and quantify the association between the SNAP-tag fusion protein and the TOMM20 protein, which is a component of the mitochondrial outer membrane translocase complex. The device utilizes fluorescence-based detection methods to measure the interaction between these two proteins.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (4 mentions)
Lamp1 gfp, by Addgene (2 mentions)
Mruby clathrin, by Addgene (2 mentions)
Pegfp sec23a, by Addgene (2 mentions)
Zsgreen rab5, by Addgene (2 mentions)
Egfp rab7a, by Addgene (2 mentions)
Psnapf cox8a, by Addgene (2 mentions)
Nunc glass bottom dishes, by Thermo Fisher Scientific (2 mentions)
Emtb 3xgfp, by Addgene (2 mentions)
2 protocols using psnapf tomm20
1
Fluorescent Protein Transfection Workflow
2
Fluorescent protein-tagged cell imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were grown overnight in 24-well plates at 37°C in a 5% CO 2 atmosphere. After reaching over 80% confluence, the plasmids mRuby-Clathrin (Addgene #55852), pEGFP-Sec23A (Addgene #66609), ZsGreen-Rab5 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.), EGFP-Rab7A (Addgene #28047), GFP-LAMP1 (Addgene #16290), EMTB-3XGFP (Addgene #26741), pSNAPf-Cox8A (Addgene #101129), or pSNAPf-TOMM20 (custom synthesized by Genomeditech (Shanghai, China) Co., Ltd.) was transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. After 24 h, the transfected cells were digested with trypsin-EDTA and seeded into Nunc Glass Bottom Dishes (Φ 12 mm, Thermo Fisher Scientific, Inc.) at a density of 1.5~2.0 × 104 per well in growth medium (150 µL). The cells were grown for an additional 12~24 h before incubation with the indicated probes.
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