Mice were anesthetized with chloral hydrate (100 mg/kg) and transcardially perfused with ice-cold PBS/heparin (50 u/ml) solution followed by 10 % formalin. Brains were then post-fixed for 72 hrs at 4 °C in 10 % formalin and then sliced using a VT1000S vibratome (Leica). Forty-micron coronal sections were collected in 6 pools, each containing16-18 slices, spanning the entire rostral-caudal axis of the hippocampus. Morphological analysis was done by incubating one pool of slices with rabbit anti Iba1 1:1000 (Wako, Cat. #019-19741) overnight at 4 °C. To assess PSD95 and CD68 staining, a second pool of slices was incubated with rabbit anti Iba1 1:500 (Wako, Cat. #019-19741), PSD95 1:100 (Merck-Millipore, Cat. # MAB1596), and CD68 1:400 (BioRad, Cat. # MCA1957T), followed by fluorescently labeled secondary antibodies (ThermoFisher, 1:400). Stained slices were then mounted on glass slides with VECTASHIELD HardSet antifade mounting medium with DAPI (Vector laboratories Cat# 10955).
Mca1957t
Manufactured by Thermo Fisher Scientific
The MCA1957T is a laboratory instrument designed for general-purpose analytical applications. It features a microcontroller-based architecture and provides users with various measurement capabilities. The specific details and intended use of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Fluorescently labeled secondary antibody, by Thermo Fisher Scientific (2 mentions)
Vt1000s vibratome, by Leica (2 mentions)
Vectashield hardset antifade mounting medium with dapi, by Vector Laboratories (2 mentions)
Rabbit anti iba1, by Fujifilm (2 mentions)
Psd95, by Fujifilm (2 mentions)
Mab1596, by Bio-Rad (2 mentions)
2 protocols using mca1957t
1
Hippocampal Immunohistochemistry in Mice
2
Hippocampal Immunohistochemistry in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were anesthetized with chloral hydrate (100 mg/kg) and transcardially perfused with ice-cold PBS/heparin (50 u/ml) solution followed by 10 % formalin. Brains were then post-fixed for 72 hrs at 4 °C in 10 % formalin and then sliced using a VT1000S vibratome (Leica). Forty-micron coronal sections were collected in 6 pools, each containing16-18 slices, spanning the entire rostral-caudal axis of the hippocampus. Morphological analysis was done by incubating one pool of slices with rabbit anti Iba1 1:1000 (Wako, Cat. #019-19741) overnight at 4 °C. To assess PSD95 and CD68 staining, a second pool of slices was incubated with rabbit anti Iba1 1:500 (Wako, Cat. #019-19741), PSD95 1:100 (Merck-Millipore, Cat. # MAB1596), and CD68 1:400 (BioRad, Cat. # MCA1957T), followed by fluorescently labeled secondary antibodies (ThermoFisher, 1:400). Stained slices were then mounted on glass slides with VECTASHIELD HardSet antifade mounting medium with DAPI (Vector laboratories Cat# 10955).
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