Water cooled low speed diamond saw

Manufactured by Buehler

Sourced in United States

The water-cooled low-speed diamond saw is a laboratory equipment designed for precision cutting of materials. It utilizes a diamond-tipped blade and a water-cooling system to facilitate controlled and accurate cuts. The core function of this saw is to provide a safe and efficient way to section samples for further analysis or preparation.

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Lab products found in correlation

Sm2500, by Leica (1 mentions)

Spelling variants of this product

Low speed saw (8 citations) Low-speed diamond saw (4 citations) Water-cooled diamond saw (2 citations) Diamond saw (2 citations)

3 protocols using water cooled low speed diamond saw

Dental Tissue Extraction and Preparation

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A detailed description of all methods used is included in the supplementary information. Statistical analysis methods are described in each section.
Human teeth and saliva used in this study were obtained according to University of Toronto Human Ethics Protocol #25793. Teeth (3^rd molars) were extracted and were handled as before [35 ], stored in tap water at −20°C until use. Cutting of tooth structure was performed with a water-cooled low-speed diamond saw (Buehler Ltd., Lake Bluff, IL). Enamel coronal edges were removed, and coronal dentin slabs were taken from within 2 mm of the cervical line plane before using for various experiments below. Photopolymerization of resins was carried out with a plasma arc device (Sapphire Plus Plasma Arc Curing System, Dent Mat, Santa Maria, CA) for 10 s (adhesive layer) or 20 s (per 1 mm layer of resin composite) at a distance of approximately 1 mm and a minimum intensity of 1730 mW cm⁻² as verified by the device’s internal radiometer [17 (link)].

Stewart C.A., Hong J.H., Hatton B.D, & Finer Y. (2020). Antimicrobial antidegradative dental adhesive preserves restoration-tooth bond. Dental materials : official publication of the Academy of Dental Materials, 36(12), 1666-1679.

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Immature Premolar Root Dentin Preparation

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Ten immature human mandibular premolars previously stored in 0.1% thymol at 4°C were used within 6 months of extraction after obtaining Indiana university Institutional Review Board approval to use human teeth (IRB number; 1305011353). The inclusion criteria were the absence of caries, root cracks, or restorations. Furthermore, each immature tooth had to have at least a 1 mm-diameter opening at the apical foramen with two-thirds of the root formed. Each tooth was decoronated, and two 4 mm root dentin cylinders were obtained from the coronal and middle thirds of each root utilizing a water-cooled low-speed diamond saw (Buehler, Lake Bluff, IL, USA). The pulp tissue was extirpated with a barbed broach without touching the root canal surface. Four specimens were obtained from each root by sectioning each cylinder longitudinally across the maximum diameter of the root canal into two specimens without touching the root canal dentin surface.

Yassen G.H., Al-Angari S.S, & Platt J.A. (2014). The use of traditional and novel techniques to determine the hardness and indentation properties of immature radicular dentin treated with antibiotic medicaments followed by ethylenediaminetetraacetic acid. European Journal of Dentistry, 8(4), 521-527.

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Preparation of Dentin Films from Third Molars

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Non-carious, un-erupted human third molars were collected without associated patient identifiers, and the collection protocol was determined not to be human subject research (NHSR 12-50) by the University Adult Heath Sciences Institutional Review Board. The teeth were stored in 0.96% PBS containing 0.002% sodium azide at 4 °C before use. Roots of teeth were removed 2–3 mm below the cementoenamel junction using a water-cooled low-speed diamond saw (Buehler, Lake Bluff, IL, USA). The remaining tooth was then attached to an aluminum disc by using cyanoacrylate adhesive (Zapit, Dental Ventures of America, Corona, CA, USA). The occlusal 1/3 of the crown and surrounding enamel was removed to result in a dentin block. The 10-µm-thick dentin films were cut from the superficial dentin portion on the block using a microtome (Leica SM2500S, Deerfield, IL, USA). Fifty dentin films were acquired from each tooth for a total of around 400 films with a size of approximately 6 mm × 5 mm when pooled together. The films were stored at 4 °C in 0.96% PBS containing 0.002% sodium azide.

Wang Y., Green A., Yao X., Liu H., Nisar S., Gorski J.P, & Hass V. (2021). Cranberry Juice Extract Rapidly Protects Demineralized Dentin against Digestion and Inhibits Its Gelatinolytic Activity. Materials, 14(13), 3637.

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