Fl 4600 spectrofluorimeter

Manufactured by Hitachi

Sourced in Japan

The FL-4600 spectrofluorimeter is a laboratory instrument designed for the measurement of fluorescence properties of samples. It is capable of recording emission and excitation spectra, as well as performing time-resolved fluorescence measurements. The device utilizes a xenon lamp as the excitation source and a high-sensitivity photomultiplier tube detector to provide accurate and reliable fluorescence data.

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Lab products found in correlation

Inova 400 mhz, by Agilent Technologies (1 mentions) Uv 2550 uv visible spectrophotometer, by Shimadzu (1 mentions) Multimode nanoscope 5, by Veeco (1 mentions) Jem 2010, by JEOL (1 mentions) Equinox 55 ft ir spectrometer, by Bruker (1 mentions)

2 protocols using fl 4600 spectrofluorimeter

Synthesis and Characterization of Organic Compounds

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All reactants were commercially available and used without further purification. Melting points were recorded on electrothermal digital melting point apparatus and were uncorrected. ¹H and ¹³C NMR spectra were recorded at 295 K on a Varian INOVA 400 MHz or a Varian NMR System 300 MHz spectrometer using CDCl₃ or d₆-DMSO as solvent and TMS as internal standard. UV-vis spectra were recorded on a Shimadzu UV-2501PC spectrometer; fluorescence spectra were obtained on an Hitachi FL-4600 spectrofluorimeter; HRMS data were measured using TOF-MS(EI+) instrument. The synthetic routines are shown in Scheme 1.

Liu Y.C., Lu G.D., Zhou J.H., Rong J.W., Liu H.Y, & Wang H.Y. (2022). Fluoranthene dyes for the detection of water content in methanol. RSC Advances, 12(12), 7405-7412.

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Characterization of Functionalized Graphene

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Atomic force microscope (AFM) images of rGO-PVP and rGO-PVP-RGD were taken on a MultiMode Nanoscope V scanning probe microscopy (SPM) system (Veeco, USA). Commercially available AFM cantilever tips with a force constant of ~48 N m⁻¹ and resonance vibration frequency of 330 kHz were used. The scanning rate was set at 0.7 to 1 Hz. Transmission electron microscopy (TEM) images were acquired using a JEM-2010 (JEOL Ltd., Japan) operated at an accelerating voltage of 200 kV. UV-Vis spectra were measured at 20 °C using a UV-2550 UV-Visible spectrophotometer (Shimadzu, Japan) equipped with a 10-mm quartz cell, where the light path length was 1 cm. Fluorescence spectra were recorded on a FL-4600 spectrofluorimeter (Hitachi, Japan). The Fourier transform infrared (FTIR) spectra were recorded on a Bruker EQUINOX 55 FTIR Spectrometer.

Huang P., Wang S., Wang X., Shen G., Lin J., Wang Z., Guo S., Cui D., Yang M, & Chen X. (2015). Surface Functionalization of Chemically Reduced Graphene Oxide for Targeted Photodynamic Therapy. Journal of biomedical nanotechnology, 11(1), 117-125.

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