The rat cardiomyoblast cell line, H9c2, and 293T cells were purchased from BeNa Culture Collection. Cells were maintained in DMEM (Hyclone; GE Healthcare Life Sciences) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C and 5% CO2 in an incubator.
293t cells
Manufactured by BNCC
Sourced in China
293T cells are a widely used line of human embryonic kidney cells that have been modified to express the SV40 large T antigen. These cells are commonly used in various research and biotechnology applications due to their high transfection efficiency and ability to produce high levels of recombinant proteins.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Pmirglo vector, by Promega (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Ipec j2, by BNCC (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
5 protocols using 293t cells
1
H9c2 and 293T Cell Cultivation
2
Validation of miR-30d-5p Target Genes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The target gene verification was conducted by dual-luciferase reporter assay. MiR-30d-5p mimic/mimic control and the pmirGLO vector (E1330, Promega, USA) containing the wild-type (WT) or mutant (MUT) 3′UTR region of BECN1 or XIST were cotransfected into 293T cells (BNCC353535, BeNa Culture Collection, China) using Lipofectamine 2000 Transfection Reagent. Post 48 h transfection, the activities of firefly luciferase and Renilla luciferase were measured using the Dual-Luciferase® Reporter Assay System (E1910, Promega, USA).
3
CDC42 gene 3'UTR amplification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The liver tissue samples were collected from three male landrace at six months and stored at −80°C until RNA extraction and as a template for CDC42 gene 3′UTR amplification. The 293T cells and IPEC-J2 were purchased from BeNa Culture Collection (BNCC, Beijing, China). The cells were cultured in DMEM/F12 medium (HyClone, New York, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Inc., New York, USA), and 1% penicillin-streptomycin at 37°C and 5% CO2. When cell confluence reached 70% to 80%, the transfection is carried out.
4
Glucose-Induced Modeling of Diabetic Nephropathy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glomerular mesangial cells (HGMCs) and human renal glomerular endothelial cells (HRGECs) were obtained from ScienCell Research Laboratories (Carlsbad, CA, USA). 293 T cells were bought from BeNa Culture Collection (Beijing, China). Cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (Invitrogen) at 37℃ with 5% CO2. HGMCs and HRGECs were stimulated with 5.5 mM glucose (normal group) or 30 mM glucose (high glucose group). High glucose-induced HGMCs and HRGECs were served as models of DN cells.
5
Establishing HMCs Diabetic Nephropathy Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human glomerular mesangial cells (HMCs) and 293T cells were acquired from BeNa Culture Collection (Beijing, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) medium (Gibco, Carlsbad, CA, USA) plus 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Sigma, St. Louis, MO, USA) at 37 °C with 5% CO2. HMCs exposed to high glucose (HG; final concentration of 25 mM; Sigma) or normal glucose (NG; final concentration of 5.5 mM) for 48 h were utilized as DN cell model.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!