At one month post-surgery, mice were re-anesthetized and placed in a magnetic resonance imaging (MRI)-compatible holder. Structural MRI data were collected using a Bruker 7 T preclinical scanner with a 72 mm volume coil and a phased array mouse brain coil, as previously described 31 . In brief, axial diffusivity (AD), mean diffusivity (MD), fractional anisotropy (FA), and radial diffusivity (RD) maps were generated using the Bruker software PARAVISION 5.0 and processed for region of interest (ROI) analysis (Figure S2 A). White matter ROIs, including corpus callosum, fimbria, internal capsule, anterior commissure, and optic tract, were selected from within T2-weighted structural volumes by an observer blinded to intervention, and then transferred onto the parametric maps for measurements.
72 mm volume coil
Manufactured by Bruker
Sourced in Germany
The 72 mm volume coil is a core component used in Bruker's lab equipment. It serves as a radio frequency (RF) coil for signal transmission and reception in various applications. The 72 mm specification refers to the diameter of the coil.
Automatically generated - may contain errors
Lab products found in correlation
9.4t mri, by Bruker (2 mentions)
7t pre clinical scanner, by Bruker (1 mentions)
Paravision 5, by Bruker (1 mentions)
9.4 t horizontal scanner, by Bruker (1 mentions)
Magnevist, by Bayer (1 mentions)
Biospin spectrometer, by Bruker (1 mentions)
Matlab, by MathWorks (1 mentions)
Paravision 5, by Rapid Biomedical (1 mentions)
10 protocols using 72 mm volume coil
1
Structural MRI Analysis of White Matter in Mice
2
MRI Tracking of Magneto-EVs In Vivo
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal studies were performed on a 11.7T Biospec (Bruker) horizontal bore scanner equipped with a mouse brain surface array RF coil (receiver) and a 72 mm volume coil (transmitter). A 30‐min dynamic scan was acquired using a fast low angle shot (FLASH) gradient echo sequence immediately before i.v. injection of 1 × 109 magneto‐EVs or 10 ng SPIO‐His (having the same iron amount as that in magneto‐EVs) in 200 μl PBS. The acquisition parameters were: flip angle = 25°, TR = 800 ms, TE = 5.8 ms, matrix size = 256 × 128 and resolution = 167 × 280 mm2. Before and 30 min after injection T2* maps were also acquired using a multiple gradient echo (MGE) pulse sequence with TR = 800 ms and TE times of 2.6, 5.8, 9, 12.2, 15.4, 18.6, 21.8, and 25 ms.
After MRI, mice were euthanized by cervical dislocation under anaesthesia and tissues of interest were collected and fixed in 4% paraformaldehyde solution for ex vivo MRI and histological analysis.
After MRI, mice were euthanized by cervical dislocation under anaesthesia and tissues of interest were collected and fixed in 4% paraformaldehyde solution for ex vivo MRI and histological analysis.
3
In Vivo Rat MRI Experiments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All studies were performed on a 4.7-T MRI system (Bruker BioSpin, Billerica, MA) using a 72-mm volume coil (Bruker) and gradient strength up to 38 G/cm. In vivo rat experiments were performed in accordance with protocols approved by the Institutional Animal Care and Use Committee of the Korea Basic Science Institute (KBSI-IACUC, Ochang, Korea).
4
High-resolution MRI of Rat Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All imaging experiments were performed on a Bruker 9.4T horizontal scanner using a 72 mm volume coil (Bruker, Billerica, MA). Adult and aged SHAM and ME rat brains were randomly assigned to imaging tubes. For each tube of brains, T2-weighted images were first acquired at 161 micron isotropic resolution (echo time [TE] = 25 ms, matrix 256×512, 12 averages, ∼16 hours scan time). DTI images were acquired using a spin-echo based sequence with 200 micron isotropic resolution (TE = 26.9 ms, TR = 27.5 s, matrix size of 256×128, ∼55–60 axial slices, 64 gradient directions with b = 2000 s/mm2, 3 images with b = 0, scan time = ∼61 hours).
5
CE-MRI Imaging of Human Atrial Anatomy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the functional mapping experiment, the human atria were formalin fixed and kept at 4°C. To prepare the heart for CE‐MRI, it was washed out with phosphate buffered saline and then incubated at 4°C in 0.2% Gd‐DTPA (dimeglumine gadopentetate Magnevist; Bayer Schering Pharma) for 7 days as previously described for human LRA.8 CE‐MRI was performed using a 9.4 T Bruker BioSpin Spectrometer (Ettlingen, Germany) and a 72‐mm volume coil at a resolution of 180×180×360 μm3. CE‐MRI images of the human atria (107×61×85 mm3) were interpolated to an isotropic resolution of 180 μm3, segmented, and smoothed using a custom Matlab program (MathWorks) and visualized in 3D using Amira (FEI Company) (Figure 1 and Figure S1 ).23 , 24 Optical maps and the reconstructed 3D human atrial model were reconciled using atrial anatomical landmarks.8
6
MRI Artifact Evaluation of FSCV Electrode
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All MRI data were acquired using a Bruker 9.4 T MRI. In vitro scans used a Bruker 72 mm volume coil. An FSCV electrode was embedded within an agarose phantom and scanned to examine whether the materials induced artifacts in RARE (rapid acquisition with relaxation enhancement; BW=312.5 kHz, TR=2000 ms, TE=40 ms, in-plane resolution= 100 μm, slice thickness=100 μm) or FLASH (fast low angle shot; BW=312.5 kHz, TR=50 ms, TE=3.52 ms, in-plane resolution= 100 μm, slice thickness=100 μm).
7
Preclinical MRI Imaging of Mouse Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse MRI data were acquired by using a 7T horizontal bore preclinical MRI system with a 72-mm volume coil (Bruker BioSpin MRI GmbH, Germany; software: ParaVision 5.1), and a 4-channel array rat brain receiver coil for signal reception (RAPID Biomedical GmbH, Germany). Animals were imaged in supine position and kept under anesthesia using air, oxygen and isoflurane (2-3%, Isoba vet., Schering-Plough Animal-health, Denmark). A circulating warm-water and heating-pad system maintained the body temperature, and a pressure-sensitive pad monitored breathing (SA Instruments, Inc., NY). Anatomical MRI neck scans were acquired using a T2-weighted 2D RARE sequence with fat suppression (Repetition time: 4500 ms, Echo time: 35 ms, Number of averages: 16, Turbo factor: 6, Slice thickness/gap: 0.6/0.6 mm, Number of slices: 40, Pixel dimensions: 160×160 µm2, Field-of-view: 2.3×1.6 cm2). Following 1st and 2nd order automatic shimming, diffusion weighted images (DWIs) were acquired using the SE-EPI sequence (Repetition time: 3000 ms, Echo time: 21 ms, Number of averages: 3, Slice thickness/gap: 1.0/1.5 mm, Number of slices: 3, Pixel dimensions: 311×318 µm2, Field-of-view: 2.8×1.5 cm2: b-values: 0, 5, 10, 20, 35, 50, 75, 100, 200, 400, 600 and 800 s/mm2: bandwidth: 300 kHz). Apparent diffusion coefficient (ADC) maps were created online using mono-exponential fitting to data from all b-values.
8
Cuprizone-Induced Demyelination Assessed by MRI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Magnetic resonance imaging (MRI) measurements for the cuprizone model
were performed at the 4-week time point using a 7T preclinical MRI
scanner (Biospin, Bruker, Billerica, MA) with a 72-mm volume coil
(Bruker, Billerica, MA) and a 10-mm quadrature surface coil (Bruker,
Billerca, MA) served as the receiver. Multislice multi-echo
T2-weighted (T2W) images were acquired using a rapid acquisition with
relaxation enhancement sequence (TR/TE = 2500 ms/10 ms, 8 echoes, echo
spacing = 10 ms, number of excitations = 2), and T2 maps were
calculated using a single exponential decay. To calculate
magnetization transfer (MT) ratio (MTR) maps, fast low angle shot
(FLASH) images (TR/TE = 70 ms/6 ms, 10° flip angle, number of
excitations = 48) were acquired both with and without an MT saturation
pulse (Gaussian shaped, 10.25 ms long, 10 µT peak power, 6 kHz
offset). MTR and T2W images were acquired with 0.75-mm slice thickness
(six coronal slices), 25-mm field-of-view, and matrix size of
256 × 256. For data analysis, an ROI was drawn around the visible
corpus callosum structure across the six coronal slices in the MTR
maps to calculate the corpus callosum area and the MTR values. The
same ROIs were overlaid on the T2 maps to calculate the T2 values. The
individual slices from each animal were grouped together for
statistical comparisons between the two groups.
were performed at the 4-week time point using a 7T preclinical MRI
scanner (Biospin, Bruker, Billerica, MA) with a 72-mm volume coil
(Bruker, Billerica, MA) and a 10-mm quadrature surface coil (Bruker,
Billerca, MA) served as the receiver. Multislice multi-echo
T2-weighted (T2W) images were acquired using a rapid acquisition with
relaxation enhancement sequence (TR/TE = 2500 ms/10 ms, 8 echoes, echo
spacing = 10 ms, number of excitations = 2), and T2 maps were
calculated using a single exponential decay. To calculate
magnetization transfer (MT) ratio (MTR) maps, fast low angle shot
(FLASH) images (TR/TE = 70 ms/6 ms, 10° flip angle, number of
excitations = 48) were acquired both with and without an MT saturation
pulse (Gaussian shaped, 10.25 ms long, 10 µT peak power, 6 kHz
offset). MTR and T2W images were acquired with 0.75-mm slice thickness
(six coronal slices), 25-mm field-of-view, and matrix size of
256 × 256. For data analysis, an ROI was drawn around the visible
corpus callosum structure across the six coronal slices in the MTR
maps to calculate the corpus callosum area and the MTR values. The
same ROIs were overlaid on the T2 maps to calculate the T2 values. The
individual slices from each animal were grouped together for
statistical comparisons between the two groups.
9
Manganese-Enhanced MRI of Rat Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The anatomical images of the rat brain were acquired 24 h after the contrast agent
administration as described in a previous study [13 (link)]. Isoflurane was used to maintain the anesthetization (induction 4% and
maintenance 2%) for MEMRI. The rats were placed on an experimental cradle, and warm water
went through the pipe underneath the cradle to prevent temperature decline. Regarding
physiological monitoring, the stability of breathing was observed by respiration sensor.
MRI were performed using a 4.7T MRI system (Bruker, BioSpec 47/40, Karlsruhe, Germany). A
rat brain surface coil as RF receiver and the 72 mm volume coil as RF transmitter were
used (Bruker, Biospin, Rheinstetten, Germany). For analyzing the contrast agent
distribution, a set of continuous two dimensional (2D) multi-slice T1-weighted
images using spin-echo pulse sequence were acquired. T1-weighted images were
performed with the following imaging parameters; repetition time=400 ms, echo time=10.5
ms, number of averages=8, number of slices=24, slice thickness=0.5 mm, flip angle=90°,
field of view=width 40 × length 30 mm2, matrix size=256 × 256, leading to a
voxel size of 0.156 × 0.117 × 0.5 mm3.
administration as described in a previous study [13 (link)]. Isoflurane was used to maintain the anesthetization (induction 4% and
maintenance 2%) for MEMRI. The rats were placed on an experimental cradle, and warm water
went through the pipe underneath the cradle to prevent temperature decline. Regarding
physiological monitoring, the stability of breathing was observed by respiration sensor.
MRI were performed using a 4.7T MRI system (Bruker, BioSpec 47/40, Karlsruhe, Germany). A
rat brain surface coil as RF receiver and the 72 mm volume coil as RF transmitter were
used (Bruker, Biospin, Rheinstetten, Germany). For analyzing the contrast agent
distribution, a set of continuous two dimensional (2D) multi-slice T1-weighted
images using spin-echo pulse sequence were acquired. T1-weighted images were
performed with the following imaging parameters; repetition time=400 ms, echo time=10.5
ms, number of averages=8, number of slices=24, slice thickness=0.5 mm, flip angle=90°,
field of view=width 40 × length 30 mm2, matrix size=256 × 256, leading to a
voxel size of 0.156 × 0.117 × 0.5 mm3.
10
MRI Artifact Evaluation of FSCV Electrode
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!