Nano 300 zs zetasizer

Manufactured by Malvern Panalytical

Sourced in United Kingdom

The Nano 300 ZS Zetasizer is a dynamic light scattering (DLS) instrument used to measure the size and size distribution of particles, molecules, and other materials in suspension or solution. It determines the hydrodynamic size of particles through the analysis of the Brownian motion of the particles. The Nano 300 ZS Zetasizer can measure particle sizes ranging from 0.3 nanometers to 10 micrometers.

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Lab products found in correlation

Cryoplunge freezer, by Ametek (1 mentions) Nanoassemblr ignite, by Precision Nanosystems (1 mentions) Amicon ultra centrifugal filter unit, by Merck Group (1 mentions) Quant it ribogreen rna assay kit, by Thermo Fisher Scientific (1 mentions) Pni formulation buffer, by Precision Nanosystems (1 mentions) Optima xpn 100, by Beckman Coulter (1 mentions)

5 protocols using nano 300 zs zetasizer

Cryogenic TEM and SAXS Characterization of Polymer Formulations

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For cryogenic transmission electron microscopy, 4–5 μL of each formulation was applied to a 400-mesh lacy carbon copper grid. Specimens were then plunge-frozen with a Gatan Cryoplunge freezer. These specimens were imaged using a JEOL 3200FS transmission electron microscope operating at 300 keV at 4000× nominal magnification. All images were collected in vitreous ice using a total dose of ∼10 e⁻ Å⁻² and a nominal defocus range of 2.0–5.0 μm.
Small angle X-ray scattering (SAXS) studies were performed at the DuPont-Northwestern-Dow Collaborative Access Team (DND-CAT) beamline at Argonne National Laboratory’s Advanced Photon Source (Argonne, IL, USA) with 10 keV (wavelength λ = 1.24 Å) collimated X-rays. SAXS was performed on undiluted 15 mg/mL polymer formulations, as described previously. Model fitting was performed using SASView and the built-in polymer micelle model.
Dynamic light scattering measurements (DLS) were performed on 15 μg/mL polymer formulations using a Nano 300 ZS zetasizer (Malvern Panalytical, Malvern, UK), using the number average distribution for calculation of the mean diameter and polydispersity of the formulations.

Allen S.D., Liu Y.G., Kim T., Bobbala S., Yi S., Zhang X., Choi J, & Scotta E.A. (2019). Celastrol-loaded PEG-b-PPS nanocarriers as an anti-inflammatory treatment for atherosclerosis. Biomaterials science, 7(2), 657-668.

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Nanocarrier Characterization Techniques

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The nanocarrier size (z-average diameter) and polydispersity were measured using dynamic light scattering (DLS) using a Nano 300 ZS Zetasizer (Malvern Panalytical, Malvern, U.K.). BCN and PLGA morphology was visualized with cryogenic transmission electron microscopy (cryo-TEM) as previously detailed (Shang et al., 2018 (link)). Transmission electron microscopy (TEM) was performed to image BCN using uranyl acetate as a negative stain as previously reported (Bobbala et al., 2020 (link)). BCN aggregate structure and internal morphology were characterized with SAXS. These studies were performed at the DuPont-Northwestern-Dow Collaborative Access Team beamline at Argonne National Laboratory’s Advanced Photon Source with 10 keV (wavelength λ=1.24 Å) collimated X-rays, as described previously (Allen et al., 2018 (link)).

Morgun E., Zhu J., Almunif S., Bobbala S., Aguilar M.S., Wang J., Conner K., Cui Y., Cao L., Seshadri C., Scott E.A, & Wang C.R. (2023). Vaccination with mycobacterial lipid loaded nanoparticle leads to lipid antigen persistence and memory differentiation of antigen-specific T cells. eLife, 12, RP87431.

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Characterizing Nanocarrier Morphology

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The nanocarrier size (z-average diameter) and polydispersity was measured using dynamic light scattering (DLS) using a Nano 300 ZS Zetasizer (Malvern Panalytical, Malvern, U.K.). BCN and PLGA morphology was visualized with cryogenic transmission electron microscopy (cryoTEM) as previously detailed [21 (link)]. Transmission electron microscopy (TEM) was performed to image BCN using uranyl acetate as a negative stain as previously reported [22 (link)]. BCN aggregate structure and internal morphology was characterized with small angle X-ray scattering (SAXS). These studies were performed at the DuPont-Northwestern-Dow Collaborative Access Team beamline at Argonne National Laboratory’s Advanced Photon Source with 10 keV (wavelength λ = 1.24 Å) collimated X-rays, as described previously[81 (link)].

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Lipid Nanoparticle Encapsulation of circRNAs

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The circRNAs were encapsulated with lipid nanoparticles (LNPs) according to a previously described process (Ickenstein and Garidel, 2019 (link)). First, the circRNA was diluted with PNI Formulation Buffer (Precision NanoSystems, #NWW0043) to a final concentration of 170 μg/ml. Then, the lab-prepared or commercial LNP (Precision NanoSystems) were mixed with the circRNA solution at the volume ratio of 1:3 through the Ignite NxGen Cartridge (Precision NanoSystems, #NIT0002) using NanoAssemblr Ignite (Precision NanoSystems). Then the LNP-circRNA formulations were diluted 40-fold with 1×PBS buffer (pH 7.2∼7.4) and concentrated by ultrafiltration with Amicon® Ultra Centrifugal Filter Unit (Millipore). The concentration and encapsulation rate of circRNAs were measured by the Quant-it RiboGreen RNA Assay Kit (Invitrogen, #R11490). The size of LNP-circRNA particles was measured using dynamic light scattering on a Malvern Zetasizer Nano-ZS 300 (Malvern). Samples were irradiated with a red laser, and scattered light was detected. The results were analyzed to obtain an autocorrelation function using the software Zetasizer V7.13.

Qu L., Yi Z., Shen Y., Lin L., Chen F., Xu Y., Wu Z., Tang H., Zhang X., Tian F., Wang C., Xiao X., Dong X., Guo L., Lu S., Yang C., Tang C., Yang Y., Yu W., Wang J., Zhou Y., Huang Q., Yisimayi A., Liu S., Huang W., Cao Y., Wang Y., Zhou Z., Peng X., Wang J., Xie X.S, & Wei W. (2022). Circular RNA vaccines against SARS-CoV-2 and emerging variants. Cell, 185(10), 1728-1744.e16.

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Exosome Isolation from Plasma Samples

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Peripheral blood (5 mL) was collected separately from healthy volunteers and HCC patients. Plasma was isolated from peripheral blood by centrifugation at 3,000 × g for 10 min, and 2.5 mL plasma was prepared for exosome isolation. All centrifugation processes were performed at 4°C. First, the plasma was centrifuged at 2,000 × g for 30 min. The supernatant was collected and centrifuged again at 10,000 × g for 45 min to remove debris and microvesicles. The supernatant was filtered through a 0.22 μm membrane followed by ultracentrifugation at 110,000 × g for 2 h (Optima XPN-100; Beckman Coulter). The exosome pellet was washed in 1 mL PBS and resuspended in PBS, followed by an additional step of ultracentrifugation at 110,000 × g for 70 min.
The exosome was suspended in PBS and divided into two parts; one was used for the electron microscopy experiment (Servicebio) and the other was analyzed for particle size using a particle size analyzer (Malvern Zetasizer Nano-ZS 300).

Zhao B., Zheng X., Wang Y., Cheng N., Zhong Y., Zhou Y., Huang J., Wang F., Qi X., Zhuang Q., Wang Y, & Liu X. (2024). Lnc-CCNH-8 promotes immune escape by up-regulating PD-L1 in hepatocellular carcinoma. Molecular Therapy. Nucleic Acids, 35(1), 102125.

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