Cd45 fitc 30 f11

Manufactured by BioLegend

CD45 FITC (30-F11) is a fluorescently-labeled antibody that binds to the CD45 antigen. CD45 is a transmembrane protein tyrosine phosphatase that is expressed on the surface of most hematopoietic cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-CD45-FITC (clone 30-F11, FITC-anti-CD45 (30-F11, CD45 (30-F11, CD45-FITC

3 protocols using cd45 fitc 30 f11

Immune Cell Characterization by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The procedure used in this study has been published previously (14 (link),26 (link)). Briefly, the following anti-mouse monoclonal antibodies were purchased from BD Biosciences: Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 (1A8; 560602), Ly6C-fluorescein isothiocyanate (FITC) (AL-21; 553104), CD4-allophycocyanin (APC) (RM4-5; 553051), CD25-FITC (7D4; 553071), and CD19-APC (1D3; 550992). The following antibodies were purchased from BioLegend: CD45-FITC (30F-11;103108), CD45-PerCP-Cy5.5 (30F-11;103132), CD45-brilliant violet (BV510) (30F-11;103138), CD11b-APC/Fire750 (M1/70;101262), CD244-Alexa Fluor 647 (2B4;133509), F4/80-phycoerythrin (PE) (BM8;123110), PD-L1-BV421 (10F.9G2;124315), CD8a-APC/Fire 750 (53-6.7;100766), CD8a-PE (53-6.7; 100708), CD127-BV510 (A7R34;135033), CD1d-PE (1B1;123509), and CD5-BV421 (53-7.3;100618). The isolated immune cells were stained in a 96-round-bottom-well plate for 20 min at 4°C and washed with PBS containing 1% bovine serum albumin. Sorting of Ly6G⁺CD244⁺ and Ly6G⁺CD244⁻ cells was conducted on a FACSAria instrument (BD Biosciences). Flow cytometric data were obtained by a FACS Verse instrument (BD Biosciences) and analyzed by FlowJo software (TreeStar, Inc.). CD45-gated cells were analyzed in this study.

Sugita Y., Yamashita K., Fujita M., Saito M., Yamada K., Agawa K., Watanabe A., Fukuoka E., Hasegawa H., Kanaji S., Oshikiri T., Matsuda T., Nakamura T., Suzuki S, & Kakeji Y. (2021). CD244+ polymorphonuclear myeloid-derived suppressor cells reflect the status of peritoneal dissemination in a colon cancer mouse model. Oncology Reports, 45(6), 106.

+ Open protocol

+ Expand

Isolation of mouse hematopoietic progenitor cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse bone marrow cells were collected from the femurs, tibiae and iliac crest and depleted of red blood cells by an ammonium chloride lysis step (STEMCELL Technologies). Cells were lineage depleted using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (19856, STEMCELL Technologies). Lineage⁻ c-Kit⁺ (LK) cells were then isolated using the following antibodies (clone and company): streptavidin BV510 (BioLegend), c-kit APC-Cy7 (2B8, BioLegend), Sca1 BV421 (D7, BioLegend), CD45 FITC (30-F11, BioLegend), EPCR (CD201) PE (RMEPCR1560, STEMCELL Tech), CD150 PE/Cy7 (TC15-12F12.2, BioLegend), CD45 FITC (30-F1,1 BD Bioscience) and CD48 APC (HM48-1, eBioscience). Flow cytometry was performed on an LSRII Fortessa (BD) and all data were analyzed using FlowJo (BD). A representative gating strategy is shown in Figure S12.

Isobe T., Kucinski I., Barile M., Wang X., Hannah R., Bastos H.P., Chabra S., Vijayabaskar M.S., Sturgess K.H., Williams M.J., Giotopoulos G., Marando L., Li J., Rak J., Gozdecka M., Prins D., Shepherd M.S., Watcham S., Green A.R., Kent D.G., Vassiliou G.S., Huntly B.J., Wilson N.K, & Göttgens B. (2023). Preleukemic single-cell landscapes reveal mutation-specific mechanisms and gene programs predictive of AML patient outcomes. Cell Genomics, 3(12), 100426.

+ Open protocol

+ Expand

Isolation and Characterization of Brain Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Prior to tissue harvest, animals were anesthetized with isofluorane and intracardially perfused with cold heparinized saline. After removing the cerebellum and olfactory bulbs, brain tissue was processed immediately by fine cutting followed by digestion with an enzyme cocktail of collagenase/DNase (Sigma). Myelin was removed by density centrifugation in DMEM supplemented with 20% BSA, and the remaining cell pellet was resuspended in media for staining. Brains were processed individually, without pooling. Isolated cells were first blocked using CD16/CD32 Mouse BD Fc Block (BD Biosciences, San Jose, CA), and surface proteins were detected using monoclonal antibodies CD45 FITC (30-F11, Biolegend, San Diego, CA), CD11b BV510 (M1/70, Biolegend), CCR2 BV650 (SA203G11, Biolegend), CD3e PE (145-2C11, BD Pharmingen), CD19 PE-Cy7 (6D5, Biolegend), CD11c APC (N418, Biolegend), and Ly6c APC-Fire750 (HK1.4, Biolegend), CD74 BV421 (In1, BD). Dead cells were excluded using Live/Dead Fixable Blue (Thermo Fisher Scientific, Waltham, MA), according to manufacturer’s instructions. After staining, cells were washed and acquired using a FACSymphony (BD Scientific) equipped with FACS Diva v.8, and data were analyzed using FlowJo software v.10.0.7.

Davis C.M., Zhang W.H., Bah T.M., Roese N.E., Allen E.M., Leung P., Boutros S.J., Marzulla T., Patel E., Nie X., Alkayed F.N., Huang J.H., Jensen M.A., Raber J., Pike M.M, & Alkayed N.J. (2022). Age-dependent cognitive impairment, hydrocephalus and leukocyte infiltration in transgenic mice with endothelial expression of human EPHX2. NPJ Aging, 8(1), 9.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!