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Hc pl apo 63x 1.40 oil cs2 plan apochromat oil immersion objective

Manufactured by Leica
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The HC PL APO 63x/1.40 OIL CS2 plan apochromat oil immersion objective is a high-performance microscope objective lens designed for use with oil immersion. It has a magnification of 63x and a numerical aperture of 1.40, providing high-resolution imaging capabilities.

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Lab products found in correlation

Slide nunc lab tek, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

HC PL Apo 63x/1.40 Oil CS2 oil-Immersion objective HC PL APO 63x/1.40 OIL CS2 objective HC PL APO 63x/1.40 OIL CS2 PL Apo 63x/1.40 Oil CS2 objective HC PL APO 63x/1.40 oil objective PL APO CS2 63x/1.40 oil HC PL APO CS2 63x/1.40 Plan Apo 63x oil immersion objective Oil CS2 HC Plan Apo HC Plan Apochromat oil objective

2 protocols using hc pl apo 63x 1.40 oil cs2 plan apochromat oil immersion objective

1

Multicolor Organelle Imaging in MEFs

Check if the same lab product or an alternative is used in the 5 most similar protocols
For dual-color imaging between ER-Tracker Green, ER-Tracker Red or MitoTracker Deep Red FM with Mag-Fluo-4 AM or Rhod-2 AM, WT and Bok−/− MEFs were seeded in an 8-well Chamber Slide Nunc Lab-Tek (Thermo Fisher). After 24 hr cells were rinsed with Hank’s buffered salt solution (HBSS), and then incubated for 25 min at 37 C in HBSS prewarmed staining solution with 1 μM for ER-Tracker Green, 1 μM ER-Tracker Red or 100 nM MitoTracker Deep Red FM combined with 1 μM Mag-Fluo-4 AM or 1 μM Rhod-2 AM (supplemented with 0.02% Pluronic acid). Cells were rinsed three times in HBSS and then incubated for a further 30 min to allow complete de-esterification of intracellular AM esters. Cells were imaged using a Leica SP8 STED super resolution microscope using a HC PL APO 63x/1.40 OIL CS2 plan apochromat oil immersion objective and the following excitation LED light source/emission filters: 504/511 nm (for ER-TrackerTM Green), 587/615 nm (for ER-TrackerTM Red), 644/665 nm (for MitoTracker Deep Red FM), 490/517 nm (for Mag-Fluo-4 AM) and 552/581 nm (for Rhod-2 AM).
Carpio M.A., Means R.E., Brill A.L., Sainz A., Ehrlich B.E, & Katz S.G. (2021). BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites. Cell reports, 34(10), 108827.
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2

Multicolor Organelle Imaging in MEFs

Check if the same lab product or an alternative is used in the 5 most similar protocols
For dual-color imaging between ER-Tracker Green, ER-Tracker Red or MitoTracker Deep Red FM with Mag-Fluo-4 AM or Rhod-2 AM, WT and Bok−/− MEFs were seeded in an 8-well Chamber Slide Nunc Lab-Tek (Thermo Fisher). After 24 hr cells were rinsed with Hank’s buffered salt solution (HBSS), and then incubated for 25 min at 37 C in HBSS prewarmed staining solution with 1 μM for ER-Tracker Green, 1 μM ER-Tracker Red or 100 nM MitoTracker Deep Red FM combined with 1 μM Mag-Fluo-4 AM or 1 μM Rhod-2 AM (supplemented with 0.02% Pluronic acid). Cells were rinsed three times in HBSS and then incubated for a further 30 min to allow complete de-esterification of intracellular AM esters. Cells were imaged using a Leica SP8 STED super resolution microscope using a HC PL APO 63x/1.40 OIL CS2 plan apochromat oil immersion objective and the following excitation LED light source/emission filters: 504/511 nm (for ER-TrackerTM Green), 587/615 nm (for ER-TrackerTM Red), 644/665 nm (for MitoTracker Deep Red FM), 490/517 nm (for Mag-Fluo-4 AM) and 552/581 nm (for Rhod-2 AM).
Carpio M.A., Means R.E., Brill A.L., Sainz A., Ehrlich B.E, & Katz S.G. (2021). BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites. Cell reports, 34(10), 108827.
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