Cell counting kit 8 cck 8 assay

Manufactured by Transgene

Sourced in China

The Cell Counting Kit-8 (CCK-8) assay is a colorimetric detection kit used for the determination of cell viability and cell proliferation. The assay utilizes a water-soluble tetrazolium salt that is reduced by cellular dehydrogenases, resulting in the formation of a yellow-colored formazan dye, which can be measured spectrophotometrically.

Automatically generated - may contain errors

Lab products found in correlation

Epoch microplate, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CCK-8 assay CCK-8 kit Cell counting kit CCK-8

4 protocols using cell counting kit 8 cck 8 assay

Cell Proliferation and Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detection of cell proliferation was carried out using the Cell Counting Kit 8 (CCK8) assay (Transgen, China). In 96-well plates, 3×10³ cells were seeded per well. A total of 24h, 48h, 72h, and 96h of cell culture was followed by 3h of incubation with CCK8 at 37°C. After that, 450 nm absorbance was measured using a microplate reader. Cells transfected with vectors were inoculated on a
12-well plate (2X10⁵ cells/well) with 70%–80% confluency. After 48 h, the cell was detected by the caspase-3/ELISA (enzyme-linked immunosorbent assay) (Hcusabio, China). The caspase-3 enzyme is a marker for inflammation and apoptosis signaling, as it can regulate the destruction of DNA or cytoskeletal proteins. Each test was performed at least three times.

Qi F., Liu W., Tan B., Zhang J., Ma Y., Cao C., Ma F., Zhu B., Yang J, & Liu X. (2022). BTG2 suppresses renal cell carcinoma progression through N6-methyladenosine. Frontiers in Oncology, 12, 1049928.

+ Open protocol

+ Expand

Quantitative Analysis of UC-MSC Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

UC−MSCs in MCB and WCB were recovered and seeded into 96-well plates at a concentration of 6000 cells/well in UC−MSC medium. Cell growth was evaluated each day by Cell Counting Kit-8 (CCK-8) assay (TransGen (Beijing, China)), according to the manufacturer’s instructions.

Wang J., Gao S., Zhao Y., Fan T., Zhang M, & Chang D. (2022). Manufacture and Quality Control of Human Umbilical Cord-Derived Mesenchymal Stem Cell Sheets for Clinical Use. Cells, 11(17), 2732.

+ Open protocol

+ Expand

Proliferation Assay for VSMC Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The proliferation of VSMCs transfected with miR-199a-5p mimics or inhibitor was determined using the Cell Counting Kit-8 (CCK-8) assay (TransGen Biotech Co., Ltd.) according to the manufacturer's instructions. Cells (3×10⁴ per well) transfected with miR-199a-5p mimics or inhibitor were seeded into a 96-well plate for 24, 48 and 72 h. Subsequently, 10 µl CCK-8 Solution was added to the medium and incubated for 4 h at 37°C. The absorbance was detected at 450 nm using an Epoch microplate (Bio-Tek Instruments, Inc.).

Cao Y., Cao Z., Wang W., Jie X, & Li L. (2021). MicroRNA-199a-5p regulates FOXC2 to control human vascular smooth muscle cell phenotypic switch. Molecular Medicine Reports, 24(3), 627.

+ Open protocol

+ Expand

Cell Proliferation Measurement by CCK-8 Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was measured by using a cell-counting kit-8 (CCK-8) assay (TransGen Biotech, Beijing, China) according to the manufacturer's instructions. Transfected cells were examined at 24, 48, and 72 hours post-transfection, with 10 μL of CCK8 added to the medium and allowed to incubate for 1 hour. Absorbances were then measured at a wavelength of 450 nm.

Zhao Q., Zheng X., Guo H., Xue X., Zhang Y., Niu M., Cui J., Liu H., Luo H., Yang D., Shi Y., Huangfu H., Wen S., Wu Y., Gao W, & Wang B. (2020). Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma. Journal of Cancer, 11(18), 5329-5344.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!