Wild-type (WT) and E-FABP-/- [10 (link), 11 (link)] mice were bred and maintained at the University of Louisville Research Resource Facilities. E-FABP deficiency had no apparent effect on immune cell differentiation in vivo [12 (link)]. Mice were fed NIH-31 Modified Open Formula Mouse/Rat Irradiated Diet (Envigo 7913; Envigo, Indianapolis, IN) or Laboratory Autoclavable Rodent Diet 5010 (LabDiet, St. Lous, MO) and provided autoclaved municipal tap water to drink. Male mice 5–13 weeks old were used for experiments. Attenuated (actA-deficient) Listeria monocytogenes expressing chicken ovalbumin (referred to as Lm-OVA) was provided by John Harty and described previously [13 (link)]. Mice were infected with 5x106 Lm-OVA via tail vein injection.
Laboratory autoclavable rodent diet 5010
Manufactured by LabDiet
Sourced in United States
Laboratory Autoclavable Rodent Diet 5010 is a sterile diet formulated for laboratory rodents. The diet is designed to be autoclaved for sterilization purposes.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Cby smn cb17prkdc scid j balb c scid, by Jackson ImmunoResearch (2 mentions)
Balb c scid mice, by LabDiet (2 mentions)
Axin2creert2, by Jackson ImmunoResearch (1 mentions)
Rosa26 cag loxp stop loxp tdtomato, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
C57bl 6j b6 mice, by Jackson ImmunoResearch (1 mentions)
B6 sjl male mice, by Jackson ImmunoResearch (1 mentions)
Cf 1 female mice, by Inotiv (1 mentions)
D12450, by Research Diets (1 mentions)
D12492, by Research Diets (1 mentions)
D12451, by Research Diets (1 mentions)
18 protocols using laboratory autoclavable rodent diet 5010
1
Listeria monocytogenes Infection in Mice
2
High-Fat Diet-Induced Metabolic Changes in Mice
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Animals were handled in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Institutional Animal Care and Use Committee of National Cheng Kung University (Approval No. 107164), and the experiments were carried out in compliance with the Animal Research: Reporting of In Vivo Experiments guidelines.
Male C57BL/6N mice aged 8 weeks were purchased (BioLASCO Taiwan Co., Ltd, Taiwan) and randomly assigned to receive either a chow diet (Laboratory Autoclavable Rodent Diet 5010*; LabDiet, St. Louis, MO, USA) or a HFD (TestDiet-58Y1, 60% kcal from fat; TestDiet, St. Louis, MO, USA). The fat content of HFD was 34.9% (cholesterol, 301 ppm; linolenic acid (18:2), 4.7%; linolenic acid (18:3), 0.39%; arachidonic acid, 0.06%; omega-3 fatty acid, 0.39%; total saturated fatty acid, 13.68%; total monounsaturated fatty acid, 14%; total polyunsaturated fatty acid, 5.15%). The fat content of chow diet was 4.8% (cholesterol, 267 ppm; linoleic acid, 4.54%; linolenic acid, 0.13%; arachidonic acid, 0.02%; omega-3 fatty acid, 0.25%; total saturated fatty acid, 1.22%; total monounsaturated fatty acid, 1.2%). This ad libitum diet was maintained for 12 weeks until the end of the experiment. Twelve weeks later, animals of each group went through either protocol shown inFigure 3(a) or 5(a) .
Male C57BL/6N mice aged 8 weeks were purchased (BioLASCO Taiwan Co., Ltd, Taiwan) and randomly assigned to receive either a chow diet (Laboratory Autoclavable Rodent Diet 5010*; LabDiet, St. Louis, MO, USA) or a HFD (TestDiet-58Y1, 60% kcal from fat; TestDiet, St. Louis, MO, USA). The fat content of HFD was 34.9% (cholesterol, 301 ppm; linolenic acid (18:2), 4.7%; linolenic acid (18:3), 0.39%; arachidonic acid, 0.06%; omega-3 fatty acid, 0.39%; total saturated fatty acid, 13.68%; total monounsaturated fatty acid, 14%; total polyunsaturated fatty acid, 5.15%). The fat content of chow diet was 4.8% (cholesterol, 267 ppm; linoleic acid, 4.54%; linolenic acid, 0.13%; arachidonic acid, 0.02%; omega-3 fatty acid, 0.25%; total saturated fatty acid, 1.22%; total monounsaturated fatty acid, 1.2%). This ad libitum diet was maintained for 12 weeks until the end of the experiment. Twelve weeks later, animals of each group went through either protocol shown in
3
Comprehensive Mouse Strain Protocol
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All mice used in this study, with the exception of the Zbtb40 loss of function allele mice (described in detail below), were purchased from The Jackson Laboratory. The mice were fed Laboratory Autoclavable Rodent Diet 5010 (LabDiet, Cat no. 0001326) and had ad lib. access to food and water. The mice were maintained on a 12hr:12hr light:dark cycle. The stock numbers for the strains used were as follows: B6;129S-Wnt4tm1.1Bhr/BhrEiJ (Wnt4 floxed mice, #007032, [68 (link)]), B6.Cg-Tg(Prrx1-cre)1Cjt/J (Prrx1-cre driver strain, # 005584), BALB/cJ (#000651), DBA/2J (#000671), FVB/NJ (#001800), RIIIS/J (#000683), BTBR_T+_Itpr3tf/J (#002282), SJL/J (#000686), NZW/LacJ (#001058), C57BL/6J (#00064), BPN/3J (#003004), TALLYHO/JngJ (#005314), NZO/HlLtJ (#002105), NON/ShiLtJ (#002423), NOR/LtJ (#002050), A/J (#000646), LG/Jm(#000675), NOD/ShiLtJ (#001976), 129S1/SvImJ (#002448), CBA/J (#000656), DDY/JclSidSeyFrkJ (#002243), AKR/J (#000648) and C3H/HeJ (#000659). The ages and sexes of the animals used are described per experiment. Neonatal mice were euthanized by decapitation and adult mice via CO2 asphyxiation followed by cervical dislocation.
4
Mucosal and Systemic Ig Quantification
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Eight week-old female C57BL/6J mice were purchased from The Jackson Laboratory in Bar Harbor (Maine, USA). Four animals were housed in each cage in a temperature- and humidity-controlled room with alternating light/dark cycles of 12 h, with access to Laboratory Autoclavable Rodent Diet 5010 (LabDiet, St. Louis, MO) and water ad libitum. All experimental procedures were approved by the University of Louisville's Institutional Animal Care and Use Committee. Mice were acclimated for approximately one week prior to the initiation of the studies. Mouse immunization, sample collection and quantification of mucosal and systemic Igs were performed as previously described26 (link).
5
Standardized Husbandry for C57BL/6J Mice
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C57BL/6J mice acquired from Jackson Laboratory (Bar Harbor, ME, USA) were maintained at the Singhealth Experimental Medicine Centre (SEMC) facility. They were bred and housed in either the SPF or GF facility, with the temperature being maintained at approximately 23 °C under a 12-h light/12-h dark cycle. The light and dark periods started from ZT0 (7:30 am) and ZT12 (7:30 pm), respectively. The SPF mice were fed with a standard irradiated chow diet (Specialty Feeds, Perth, Australia), while GF mice were fed with an autoclaved standard chow diet (Laboratory Autoclavable Rodent Diet 5010, LabDiet, St. Louis, MO, USA). The mice were housed at a maximum of five mice per cage, with water given ad libitum. Only male mice were used in all experiments so as to eliminate confounding factors due to the estrous cycle. Mice were weighed before they were euthanized by carbon dioxide asphyxiation.
6
BALB/c SCID Mice Husbandry
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C57BL/6 and CBy.Smn.CB17PRKdc SCID/J (BALB/c SCID) mice were purchased from Jackson Laboratories (Bar Harbor). BALB/c SCID mice received Laboratory Autoclavable Rodent Diet #5010 (LabDiet). Breeding, maintenance and experimentation complied with Institutional Animal Care and Use Committee regulations.
7
Standard Housing Conditions for Mice
8
Axin2-Driven Lineage Tracing Mice
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All procedures were conducted according to the National Institutes of Health (NIH) guidelines and the Institutional Animal Care and USE Committee (IACUC) of the University of Maryland, Baltimore, protocol 0120006. Axin2CreERT2 (JAX018867), Rosa26-CAG-loxP-stop-loxP-tdTomato (Ai9:R26R-tdTomato, JAX007909), Rosa26-CAG-loxP-stop-loxP-ZsGreen (Ai6:R26R-ZsG, JAX007906), Rosa26-CAG-loxP-stop-loxP-Confetti (R26R-Confetti, JAX013731), and C57BL/6 J mice were obtained from the Jackson Laboratory. We crossed Axin2CreERT2 mice with R26R-ZsG, R26R-tdTomato or R26R-Confetti mice to create Axin2CreERT2;R26RZsGreen, Axin2CreERT2;R26RtdTomato or Axin2CreERT2;R26RConfetti mice, respectively. For Cre recombination, 120 μg·g−1 (body weight) tamoxifen (T5648; Sigma‒Aldrich) dissolved in corn oil (C8267; Sigma‒Aldrich) was subcutaneously injected for three consecutive days from P0, P23, P42 or P84 in Axin2CreERT2;R26RZsGreen mice, for three consecutive days from P23 in Axin2CreERT2;R26RtdTomato mice, and for five consecutive days from P23 in Axin2CreERT2;R26RConfetti mice. All mice were housed in specific pathogen-free conditions and were allowed free access to food (Laboratory Autoclavable Rodent Diet 5010; LabDiet) and water, except during DR, as described later.
9
BALB/c SCID Mice Husbandry
10
Mice Tissue Harvesting and RNA Analysis
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