Ifngr1

The University of Iowa institutional animal care and use committee approved all experiments. C57BL/6 WT (JAX stock 002288), Ifngr1^−/− (JAX stock 025545), and MD4 (JAX stock 002595) mice were purchased from The Jackson Laboratory. C57BL/6 Myd88^−/− and Ticam1^−/− were generous gifts from Michelle Callegan (University of Oklahoma Health Sciences Center, Oklahoma City, OK); Tim1^−/− mice were a gift from Paul Rothman (Johns Hopkins, Baltimore, MD); and Axl^−/− bone marrow was a generous gift from Carla Rothlin (Yale University, New Haven, CT). Infections in experimental mice were initiated by either serial transfer (i.v.) of 10⁶Plasmodium yoelii (Py; American Type Culture Collection) parasitized RBCs (pRBCs) derived from a single donor C57BL/6 mouse, or i.p. injections of either 2.5 × 10⁶ PFUs of LCMV-Arm or 5,000 Tb Antat1.1 parasites (Tb; a generous gift from Kent Hill, University of California, Los Angeles, Los Angeles, CA). In some experiments, Py-infected mice were treated with 500 µg of either α-IFN-γ (clone XMG1.2; BioXcell) or rat IgG1 isotype control or with 400 µg of either mouse chimeric PtS-blocking antibody clone mch1N11 or mouse IgG2a (clone 1.18.4; BioXcell) isotype control, as indicated in the figure legends. Parasitemia was measured using flow cytometry as described (Malleret et al., 2011 (link); Villarino et al., 2016 (link)).

C57BL/6 WT mice were either purchased from Charles River or bred in our specific pathogen-free facility. MHCII^−/− (H2-Aa^−/−; Köntgen et al., 1993 (link)), pI^−/−, pIV^−/− (Waldburger et al., 2003 (link)), pIII+IV^−/− (LeibundGut-Landmann et al., 2004 (link)), Ciita^−/− (Laufer et al., 1996 (link)), CD11cDOG (Hochweller et al., 2008 (link)), OT-II CD45.1⁺ (Barnden et al., 1998 (link)), K14-VEGFR3-Ig (provided by K. Alitalo, Institute of Biomedicine, University of Helsinki, Helsinki, Finland; Mäkinen et al., 2001 (link)), I-A^b GFP (Boes et al., 2002 (link); provided by A.M. Lennon-Dumenil, INSERM U932, Institut Curie, Paris, France), and Ifngr1^−/− (The Jackson Laboratory) mice have been previously described. These mouse strains are on a C57BL/6 background and were housed and maintained under SPF conditions. All animal husbandry and experiments were approved by and performed in accordance with guidelines from the Animal Research Committee of the University of Geneva. When indicated, IFN-γ (1 µg, both flanks) was injected subcutaneously. For some experiments, mice were irradiated with two consecutive doses of 300 cGy (Gammacell 40 Exactor).