IFN-γ (20ng/ml) or TNF (20ng/ml) was used to stimulate PBMCs overnight. Whole cell lysate from a patient and healthy control were prepared using cell lysis buffer. Proteins were separated by SDS-PAGE and transferred to PVDF membranes. Immunoreactive proteins were detected with a C1r antibody Ab190800 (Abcam) and were visualized using ECL Plus western blotting substrate (ThermoFisher).
Ab190800
Manufactured by Abcam
Sourced in United Kingdom
Ab190800 is a product offered by Abcam, a leading provider of high-quality reagents and tools for scientific research. The core function of this product is to serve as a tool for researchers, but no further details on its intended use can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Ecl plus western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Ab75756, by Abcam (1 mentions)
Ab195352, by Abcam (1 mentions)
Ab11861, by Abcam (1 mentions)
Ab246514, by Abcam (1 mentions)
Ab134943, by Abcam (1 mentions)
Ab189922, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Ab8245, by Abcam (1 mentions)
Ab200999, by Abcam (1 mentions)
2 protocols using ab190800
1
Immune Stimulation and Protein Analysis
2
Quantification of Protein Expression
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Proteins were extracted from cells or tissues using RIPA lysis buffer (Thermo Fisher). The protein concentration in each extract was detected using a BCA Protein Assay Kit (Beyotime). A sample of protein from each extract was separated by 10% SDS-PAGE, and the protein bands were transferred onto polyvinylidene fluoride membranes (Millipore, Darmstadt, Germany), which were subsequently blocked with 5% BSA for 2 h. Next, the membranes were incubated for 1 h with primary antibodies against METTL3 (ab195352, 1:1000, Abcam, Cambridge, UK), YTHDF2 (ab246514, 1:1000), C3 (ab200999, 1:2000), C1q (ab11861, 1:2000), C1r (ab190800, 1:3000), C1s (ab134943, 1:2000), C1qA (ab189922, 1:2000), C1qB (ab92508, 1:2000), C1qC (ab75756, 1:1000), and GAPDH (ab8245, 1:10000, Abcam), and then incubated with a secondary antibody (Abcam) for 1 h. The immunostained protein bands were detected using an enhanced chemiluminescence reagent (Thermo Fisher).
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