Igfbp1

Manufactured by R&D Systems

Sourced in United States

IGFBP1 is a protein that regulates the bioavailability and activity of insulin-like growth factors (IGFs) in the body. It functions by binding to IGFs, which can modulate their interactions with cell surface receptors.

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Lab products found in correlation

6 protocols using igfbp1

Quantifying Angiogenic Biomarkers using Luminex

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These biomarkers were measured using Luminex Screening Assay kits (R&D Systems, MN). A four-plex cocktail containing ANG1, ANG2, IGFBP1 and IGFBP3 (R&D Systems, MN, Cat. LXSAHM-04) was used to screen at 1:2 dilution IVS plasma, MMP2 and MMP9 containing 2-plex cocktail (R&D Systems, MN, Cat. LXSAHM-02) was used to screen at 1:50 dilution IVS plasma. The assay was carried out according to the manufacturer’s instructions. Plates were washed using magnetic plate separator (Luminex, Austin, Texas, Cat# CN-0269-01) and a MAGPIX instrument (EMD Millipore, Billerica, MA) was used to read plates. The results were expressed as median fluorescence intensity (MFI). A standard curve was generated for each analyte to convert MFI into corresponding protein concentration. Protein concentrations were adjusted for dilution factors used for each analyte.

Esemu L.F., Yuosembom E.K., Fang R., Rasay S., Fodjo B.A., Nguasong J.T., Kidima W., Ekali G.L., Chen J.J., Ndhlovu L., Bigoga J.D., Taylor D.W., Leke R.G, & Babakhanyan A. (2019). Impact of HIV-1 infection on the IGF-1 axis and angiogenic factors in pregnant Cameroonian women receiving antiretroviral therapy. PLoS ONE, 14(5), e0215825.

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Quantifying Growth Factors in Serum

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Serum concentrations of GH, IGF-1, IGFBP-1, and IGFBP-3 were determined by ELISA kits in accordance with the manufacturer’s instructions (GH: Millipore, MN, USA; IGF-1, IGFBP-1, and IGFBP-3: R&D, MN, USA).

Gao X., Tian F., Wang X., Zhao J., Wan X., Zhang L., Wu C., Li N, & Li J. (2015). Leucine Supplementation Improves Acquired Growth Hormone Resistance in Rats with Protein-Energy Malnutrition. PLoS ONE, 10(4), e0125023.

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Mouse and Human Serum Biomarker Analyses

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Mouse whole blood was collected in Eppendorf tubes. It was allowed to coagulate for 2 h at room temperature, centrifuged 20 min at 4,000 rpm and then stored in aliquots in PCR tubes at −80 °C until subsequent use. Whole blood from patients was collected in Vacuette Serum Clot Tubes, centrifuged 20 min at 2,100 rpm and then aliquoted into small tubes and stored at −80 °C until use. All the ELISA assays to detect mouse and human serum level of IGF1, IGFBP1, IGFBP3, C-peptide, leptin and adiponectin were purchased from R&D System except that for mouse C-peptide, which was purchased from Alpco.

Caffa I., Spagnolo V., Vernieri C., Valdemarin F., Becherini P., Wei M., Brandhorst S., Zucal C., Driehuis E., Ferrando L., Piacente F., Tagliafico A., Cilli M., Mastracci L., Vellone V.G., Piazza S., Cremonini A.L., Gradaschi R., Mantero C., Passalacqua M., Ballestrero A., Zoppoli G., Cea M., Arrighi A., Odetti P., Monacelli F., Salvadori G., Cortellino S., Clevers H., De Braud F., Sukkar S.G., Provenzani A., Longo V.D, & Nencioni A. (2020). Fasting-mimicking diet and hormone therapy induce breast cancer regression. Nature, 583(7817), 620-624.

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Expansion of Cryopreserved UCB Cells

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Cryopreserved UCB mononuclear cells (4 × 10⁵cells/ml) were suspended in serum-free Stemspan® medium (Stemcell technologies, vancouver, BC, Canada) supplied with a standard cytokine combination of 100 ng/ml SCF, 50 ng/ml FL and 100 ng/ml TPO (all three cytokines purchased from Peprotech, Rocky Hill, NJ, USA) and with individually varied doses and combinations of IGFBP1, IGFBP2, IGF2 and ANGPTL3 (these four cytokines purchased from R&D Systems, Minnneapolis, MN, USA). The cells were inoculated on a passage 3 to 5 BM-derived mesenchymal stromal cell layer and cultured in 37°C incubator for 12 days. The expanded cells were harvested at the end of 12 days and the adherent cord blood cells were detached after 1 minute of incubation at room temperature with 0.25% trypsin–ethylenediamine tetraacetic acid.

Fan X., Gay F.P., Lim F.W., Ang J.M., Chu P.P., Bari S, & Hwang W.Y. (2014). Low-dose insulin-like growth factor binding proteins 1 and 2 and angiopoietin-like protein 3 coordinately stimulate ex vivo expansion of human umbilical cord blood hematopoietic stem cells as assayed in NOD/SCID gamma null mice. Stem Cell Research & Therapy, 5(3), 71.

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Quantification of Angiogenic Factors

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To confirm the semi-quantitative results of the angiogenesis array, protein concentrations of selected pro- and anti-angiogenic factors were measured in the CM, EV-depleted CM and (lysed) EVs of DPSCs and BM-MSCs by ELISA. ELISA kits for VEGF (#DY293B), insulin-like growth factor-binding protein-1 (IGFBP-1, #DY871), TIMP-1 (#DY970), angiopoietin-1 (Angpt-1, #DY923) (R&D Systems) and IGFBP-3 (#ab217607, Abcam) were used conform the manufacturer’s guidelines. Data were normalized to the number of seeded cells.

Merckx G., Hosseinkhani B., Kuypers S., Deville S., Irobi J., Nelissen I., Michiels L., Lambrichts I, & Bronckaers A. (2020). Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles. Cells, 9(2), 312.

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Liver-specific Cpt2 Deletion in Mice

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To generate a liver-specific loss-of-function of Cpt2, we bred Cpt2^lox/lox mice (16 (link)) to albumin-Cre transgenic mice (18 (link)). Mice were housed in ventilated racks with a 14 hr light/10 hr dark cycle and fed a standard chow diet (Harlan Laboratories). All mice were euthanized at the same time of day (3p.m.). Fed mice were food deprived from 1 p.m.-3 p.m. to ensure consistent feeding patterns. For fasting studies, mice were deprived of food for 24 hours from 3p.m.-3 p.m. For the ketogenic diet studies, mice were placed on a ketogenic diet at 9 weeks of age (47 (link)). Serum was collected for all mice to measure free glycerol and TAG (Sigma), β-hydroxybutyrate (StanBio), total cholesterol, NEFA (Wako), and ALT (Sigma). Fgf21, Gdf15, Igfbp1, Corticosterone, Adiponectin (R&D systems) and insulin (Millipore) were measured by ELISA. Body fat and lean mass of 9-week old mice was measured via magnetic resonance imaging analysis (QNMR EchoMRI100; Echo Medical Systems, LLC). Indirect calorimetery and metabolic cage studies were normalized to total lean mass as described (48 (link)). All procedures were performed in accordance with the NIH’s Guide for the Care and Use of Laboratory Animals and under the approval of the Johns Hopkins Medical School Animal Care and Use Committee.

Lee J., Choi J., Scafidi S, & Wolfgang M.J. (2016). Hepatic fatty acid oxidation restrains systemic catabolism during starvation. Cell reports, 16(1), 201-212.

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