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Precision brain slicer

Manufactured by Braintree Scientific
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The Precision Brain Slicer is a laboratory tool designed to accurately and consistently cut thin sections from brain tissue samples. It features a high-precision blade mechanism that enables precise control over the thickness of the slices, ensuring reliable and reproducible results for scientific research and analysis.

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Lab products found in correlation

Gentamycin, by Thermo Fisher Scientific (2 mentions) 12 well transwell plate, by Corning (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) H e and anti gfp, by Takara Bio (2 mentions) D glucose, by Merck Group (2 mentions) Methylcellulose, by Shin-Etsu Chemical (1 mentions) Take3 micro volume plate, by Agilent Technologies (1 mentions) Biotek spectrophotometer, by Agilent Technologies (1 mentions) Maxwell rsc simplyrna tissue kit, by Promega (1 mentions)

4 protocols using precision brain slicer

1

Murine Brain Slice Co-Culture Assay

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Animal studies were conducted according to protocols approved by the IACUC of the University of California, Davis. Fresh brain tissue sections from wild-type FvB mice 12–15 weeks of age were prepared under sterile conditions immediately after sacrifice using a precision brain slicer (Braintree Scientific, Inc). High quality 1mm coronal tissue slices were cut in half (sagittal plane) in dissection media (minimum essential medium with glutamine, 1% penicillin/streptomycin, 50μg/mL gentamycin (Thermo), and 4.5mg/mL D-glucose (Sigma)). The matching left and right hemisphere sections were then placed on sterile porous (0.4μm) membranes in a 12-well Transwell plate (Corning) and co-cultured with 250,000 U251 cells either expressing control pMX-GFP or pMX-Nrdp1-FLAG-IRES-GFP in brain culture media (50% dissection media, 25% Hanks’ balanced salt solution, 25% horse serum, 1% penicillin/streptomycin, 50μg/mL gentamycin, 1x non-essential amino acids (Thermo)). Media was replaced in both the upper and lower chambers of the transwell plate after 2–3 days. At 5 days, the tissue was gently washed with PBS, fixed in 10% neutral buffered formalin, and embedded in 1% agarose for stability during histological processing and paraffin-embedding. Representative 5μm thick sections about 50, 100, and 200μm deep were processed for both H&E and anti-GFP (Clontech) IHC.
Wald J.H., Hatakeyama J., Printsev I., Cuevas A., Fry W.H., Saldana M.J., Vorst K.V., Rowson-Hodel A., Angelastro J.M., Sweeney C, & Carraway KL I.I.I. (2017). Suppression of planar cell polarity signaling and migration in glioblastoma by Nrdp1-mediated Dvl polyubiquitination. Oncogene, 36(36), 5158-5167.
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2

IEGs Expression Analysis in Mouse Brain

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For IEGs expression analysis, ICR mice were used unless otherwise noted and habituated appropriately (as described above). Only for MAP and PCP dose-response experiments, C57BL/6J mice were used for experiments. Drugs were administered orally (p.o., suspended in 0.5% methylcellulose (Shin-Etsu Chemical)), intraperitoneally (i.p., dissolved in saline, sterile 0.9% NaCl (Otsuka Pharmaceutical Factory)) and subcutaneously (s.c., dissolved in saline same as the case of i.p.). At each time point, the mice were euthanized by decapitation and whole brains were collected. Fitting the brain on a Precision Brain Slicer (Braintree Scientific), four brain regions including prefrontal cortex, nucleus accumbens, striatum, and hippocampus were dissected on ice. Schematic representation of those brain regions is shown in S1 Fig. Here, prefrontal cortex was defined as the anterior part of the frontal lobe of the brain 1 mm behind the olfactory bulb. Nucleus accumbens, striatum, and hippocampus were dissected from both hemispheres with a 1 mm slice using a Precision Brain Slicer.
Sakuma K., Komatsu H., Maruyama M., Imaichi S., Habata Y, & Mori M. (2015). Temporal and Spatial Transcriptional Fingerprints by Antipsychotic or Propsychotic Drugs in Mouse Brain. PLoS ONE, 10(2), e0118510.
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3

Murine Brain Slice Co-Culture Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal studies were conducted according to protocols approved by the IACUC of the University of California, Davis. Fresh brain tissue sections from wild-type FvB mice 12–15 weeks of age were prepared under sterile conditions immediately after sacrifice using a precision brain slicer (Braintree Scientific, Inc). High quality 1mm coronal tissue slices were cut in half (sagittal plane) in dissection media (minimum essential medium with glutamine, 1% penicillin/streptomycin, 50μg/mL gentamycin (Thermo), and 4.5mg/mL D-glucose (Sigma)). The matching left and right hemisphere sections were then placed on sterile porous (0.4μm) membranes in a 12-well Transwell plate (Corning) and co-cultured with 250,000 U251 cells either expressing control pMX-GFP or pMX-Nrdp1-FLAG-IRES-GFP in brain culture media (50% dissection media, 25% Hanks’ balanced salt solution, 25% horse serum, 1% penicillin/streptomycin, 50μg/mL gentamycin, 1x non-essential amino acids (Thermo)). Media was replaced in both the upper and lower chambers of the transwell plate after 2–3 days. At 5 days, the tissue was gently washed with PBS, fixed in 10% neutral buffered formalin, and embedded in 1% agarose for stability during histological processing and paraffin-embedding. Representative 5μm thick sections about 50, 100, and 200μm deep were processed for both H&E and anti-GFP (Clontech) IHC.
Wald J.H., Hatakeyama J., Printsev I., Cuevas A., Fry W.H., Saldana M.J., Vorst K.V., Rowson-Hodel A., Angelastro J.M., Sweeney C, & Carraway KL I.I.I. (2017). Suppression of planar cell polarity signaling and migration in glioblastoma by Nrdp1-mediated Dvl polyubiquitination. Oncogene, 36(36), 5158-5167.
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4

Prefrontal Cortex Tissue Collection

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Animals were overdosed with urethane and rapidly decapitated. Brains were extracted, snap-frozen on dry ice, and stored at −80 °C. Slices through prefrontal cortex (1 mm) were obtained using a precision brain slicer (Braintree Scientific Inc., Braintree, MA) that was equilibrated to −20 °C. Prelimbic cortex was identified using gross anatomical landmarks (Paxinos and Watson, 1998 ) and micropunch samples (1.5 mm diameter) were obtained from both hemispheres (Fig. 1C). Total RNA was isolated using the Maxwell RSC simplyRNA Tissue Kit (Promega, Madison, WI) per the manufacturer’s recommended protocol. RNA concentration was determined using the Take3 Micro-Volume Plate and BioTek spectrophotometer (BioTek, Winooksi, VT).
Moench K.M., Breach M.R, & Wellman C.L. (2019). Prior stress followed by a novel stress challenge results in sex-specific deficits in behavioral flexibility and changes in gene expression in rat medial prefrontal cortex.. Hormones and behavior, 117, 104615.
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