Samples were prepared by pipetting 5 μL of liposomes on a Quantifoil Multi A holey carbon film supported by a copper grid (Quantifoil Micro Tools GmbH, Jena, Germany). The samples were immediately frozen by immersion into liquid ethane, then transferred under liquid nitrogen to a FEI Tecnai G2 F20 microscope (FEI Company, Hillsboro, OR). Images were captured at approximately − 170 °C with a Gatan CCD camera (Gatan Inc., Warrendale, PA) and a 200 kV acceleration voltage.
Fei tecnai g2 f20 microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FEI Tecnai G2 F20 microscope is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials. It features a field emission gun (FEG) electron source, providing a high-brightness, coherent electron beam for enhanced resolution and contrast. The microscope is capable of performing various imaging modes, including bright-field, dark-field, and high-resolution, as well as selected area electron diffraction (SAED) analysis.
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Lab products found in correlation
2 protocols using fei tecnai g2 f20 microscope
1
Liposome Cryo-TEM Imaging Protocol
2
Transmission Electron Microscopy Preparation
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All the tested samples were suspended fully in ethanol and then dropped on an ultrathin carbon stainless-steel Mo grid. The prepared samples were dried naturally under ambient conditions before placed on the TEM sample holder. The transmission electron microscopy, high-resolution transmission electron microscopy (HRTEM) images were obtained from a FEI Tecnai G2 F20 microscope (FEI Co., Hillsboro, TX, USA) operating at 200 kV with a Genesis XM2 accessory (EDAX Inc., Mahwah, NJ, USA).
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