Polystyrene co-maleic anhydride (molecular weight = ~1600), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), Hank’s balanced salt solution, Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), bovine serum albumin (BSA), and TrypLE express were bought from ThermoFisher Scientific (Dubai, UAE). L-glutamine and an antibiotic solution of penicillin/streptomycin were purchased from (Merck Hertfordshire, UK). All consumable materials such as Petri dishes, conical tubes (15 mL and 50 mL), cell culture flasks (25 cm2 and 75 cm2), and dialysis tubing were purchased from (Merck Hertfordshire, 120 Moorgate London, UK).
Antibiotic solution of penicillin streptomycin
Manufactured by Merck Group
Sourced in United Kingdom
The Antibiotic solution of penicillin/streptomycin is a laboratory product that contains a mixture of the antibiotics penicillin and streptomycin. This solution is commonly used in cell culture and microbiology applications to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Hank s balanced salt solution, by Thermo Fisher Scientific (2 mentions)
1640 medium, by Thermo Fisher Scientific (2 mentions)
Conical tube, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Polystyrene co maleic anhydride, by Thermo Fisher Scientific (2 mentions)
Petri dishes, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edac, by Thermo Fisher Scientific (1 mentions)
Dasatinib, by LC Laboratories (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Amphotericin, by HiMedia (1 mentions)
Foetal bovine serum (fbs), by Corning (1 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Gant61, by Adooq (1 mentions)
Ly294002, by Adooq (1 mentions)
4 protocols using antibiotic solution of penicillin streptomycin
1
Polystyrene-co-maleic anhydride cell culture
2
Cell Culture Reagents and Materials
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Dasatinib were retained from LC Laboratories (Woburn, MA, USA). Polystyrene co-maleic anhydride (molecular weight~1600), Roswell Park Memorial Institute (RPMI) 1640 medium, Hank’s balanced salt solution, fetal bovine serum (FBS), bovine serum albumin (BSA), and TrypLE express were bought from ThermoFisher Scientific (Dubai, United Arab Emirates). N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), L-glutamine, antibiotic solution of penicillin/streptomycin were acquired from (Merck Hertfordshire, UK). All consumable materials including petri dishes, conical tubes (15 mL and 50 mL), cell culture flasks (25 and 75 cm2), and dialysis tubing were purchased from (Merck Hertfordshire, UK).
3
Culturing Pancreatic and Fibroblast Cell Lines
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Pancreatic carcinoma MIA PaCa-2 cell line was procured from National Centre for Cell Science (NCCS), Pune, India and Human dermal fibroblast HDF cell line was procured from V.G. Vaze College, Mumbai, India. Both the cell lines were cultured in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma–Aldrich, St. Louis, MO, USA) with heat inactivated 10% foetal bovine serum (FBS) (Cell Clone, Genetix, India) and 1% antibiotic solution of penicillin–streptomycin (Sigma–Aldrich, St. Louis, MO, USA), and amphotericin (Himedia, Mumbai, India). Cells were maintained by passaging 1:3 at a regular interval of 48 h using 0.5% Trypsin-EDTA (Gibco, Paisley, UK) without phenol red. All reagents, media, buffers, chemicals and plastic-ware used were of cell culture grade.
4
Overexpression of GLI1 in AML cells
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HL60 and NB4 cells were obtained from the Cell Resource Centre (Xiangya Medical College, Central South University, Hunan, China). The cell lines were maintained in RPMI-1640 medium (Corning Inc., Corning, NY, USA) containing 10% foetal bovine serum (Corning Inc.) and 1% antibiotic solution of penicillin/streptomycin (Sigma, MO, USA) in a 37 °C incubator with a humidified atmosphere of 5% CO2 (Supplementary Fig. S2 ). To overexpress GLI1, AML cells were infected with lentivirus containing the GLI1 open reading frame (MOI: 50–100). After 72 h of infection, HL60 and NB4 lines were allowed to recover for 24 h with fresh media and were referred to as HL60/GLI1 and NB4/GLI1 cells (Supplementary Fig. S3 ). To evaluate the response to drug treatment, 1 × 106 HL60/GLI1 and NB4/GLI1 cells were seeded in 6-well culture plates and incubated with either 20 μM GANT61 (Adooq Bioscience, A13252) or 20 μM LY294002 (Adooq Bioscience, A10547) for 72 h prior to subsequent measurements.
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