The prototrophic S288C strain RCY308 was cultured and metabolites were prepared according to established procedures40 (link). Yeast metabolite extracts were reconstituted to a concentration of 0.6 OD600 of yeast in 40 uL of HPLC-grade H2O by vortexing for 1 minute. The reconstituted extract was combined with an equal volume of isotopically labeled material as an internal standard. LC-MS analysis was performed using an Agilent Technologies 6540 qTOF spectrometer equipped with a Jet Spray ESI source operated in negative ionization mode. Reverse phase separation as performed using a 2.1 mm × 100 mm Waters HSS-T3 C18 column with 1.8 um packing. Metabolite levels are presented as integrated areas relative to co-eluting isotopic reference. To ensure statistical power, a minimum of 4 biological replicates was performed for each nutritional context.
6540 qtof spectrometer
Manufactured by Agilent Technologies
The Agilent 6540 qTOF spectrometer is a high-resolution mass spectrometer designed for accurate mass measurements and compound identification. It utilizes quadrupole time-of-flight (qTOF) technology to provide precise mass information for complex samples.
Automatically generated - may contain errors
2 protocols using 6540 qtof spectrometer
1
Yeast Metabolite Extraction and LC-MS Analysis
2
Moisture-Sensitive Organic Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All air-sensitive procedures were conducted under an inert atmosphere of a nitrogen-filled dry box or by standard Schlenk techniques. All reactions were performed under an atmosphere of nitrogen unless otherwise stated. All glassware for moisture sensitive reactions was dried at 140 °C in an oven. THF and DCM were degassed by purging with argon for 45 min and dried with a solvent purification system by passing through a one-meter column of activated alumina. Flash column chromatography was performed on Fisher brand silica gel 60 (230–400 mesh). Products were visualized on TLC by UV light or by staining with KMnO4, phosphomolybdic acid or ceric ammonium molybdate. HRMS (ESI) analysis was performed at the Iowa State University Chemical Instrumentation Facility on an Agilent 6540 QTOF spectrometer. NMR spectra were acquired on Varian MR-400 and Bruker Avance III 600 spectrometers at the Iowa State University Chemical Instrumentation Facility. Chemical shifts are reported in ppm relative to a residual solvent peak (CDCl3 = 7.26 ppm for 1H and 77.0 ppm for 13C). Coupling constants are reported in hertz.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!