Western blotting experiments were performed as previously described 19 (link). The following antibodies were used: anti-Actin (Millipore, Billerica, MA, USA; cat. no. MAB1501); anti-CD99 (sc-53148), anti-Delta (sc-9102), anti-Delta-3 (sc-67270), anti-FLI1 (sc-356), anti-GAPDH (sc-25778), anti-Lamin-B (sc-6216), anti-NF-kBp65 (sc-372), anti-Notch 1 (sc-6014_R) and anti-Notch 3 (sc-5593) (Santa Cruz Biotechnology, Dallas, TX, USA); anti-Phopsho-NF-kBp65 (Ser536) (Cell Signaling Technology, Beverly, MA, USA; cat. no. 3031); anti-α-Tubulin (Sigma Aldrich; cat. no. T5168); anti-rabbit or anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare; cat. no. NA934V, NA931V) were used as secondary antibodies. Proteins were visualized by incubating with ECL (EuroClone, Milan, Italy).
Anti cd99
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-CD99 is a laboratory reagent that can be used to detect the presence of the CD99 protein, which is expressed on the surface of various cell types. It is commonly used in research applications to identify and study cells expressing CD99.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase, by GE Healthcare (1 mentions)
Anti lamin b, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti nf kbp65, by Santa Cruz Biotechnology (1 mentions)
Anti fli1, by Santa Cruz Biotechnology (1 mentions)
Anti notch3, by Santa Cruz Biotechnology (1 mentions)
Enhanced chemi luminescence (ecl), by Euroclone (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Anti ezrin, by Merck Group (1 mentions)
Radioimmunoprecipitation assay (ripa), by Thermo Fisher Scientific (1 mentions)
A32959, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using anti cd99
1
Western Blotting Antibody Validation
2
Protein Analysis of Cell and EV Lysates
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lysis of cells and EVs was performed using the radioimmunoprecipitation assay (RIPA) buffer (89900, Thermo Fisher Scientific) supplemented with protease and phosphatase inhibitors (A32959, Thermo Fisher Scientific). An equal amount of proteins were run on SDS gels under denaturing conditions and blotted onto nitrocellulose membranes. The membranes were incubated overnight at 4 °C with the following primary antibodies: anti-CD99 (sc-53148; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Ezrin (E8897; Sigma-Aldrich); anti-XAGE1A (A61802; Epigentek, Farmingdale, NY); anti-GAPDH (5174; Cell Signaling); anti-GPI (MA515396; Thermo Fisher Scientific), anti-IGFALS (sc-377131; Santa Cruz Biotechnology).
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