Anti ssea1

Manufactured by STEMCELL

Sourced in United States

Anti-SSEA1 is a lab reagent used for the detection and analysis of the SSEA-1 (stage-specific embryonic antigen-1) marker on cells. SSEA-1 is a carbohydrate epitope expressed on the surface of undifferentiated embryonic stem cells and other cell types. Anti-SSEA1 can be used in various cell biology applications, such as flow cytometry and immunocytochemistry, to identify and characterize SSEA-1 positive cell populations.

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Lab products found in correlation

Anti nanog, by Cosmo Bio (1 mentions) Anti oct4, by Santa Cruz Biotechnology (1 mentions) Anti cd29, by BioLegend (1 mentions) Anti cd34, by BioLegend (1 mentions) Anti ssea 3, by BioLegend (1 mentions) Anti cd90, by BioLegend (1 mentions) Anti cd45, by BioLegend (1 mentions)

2 protocols using anti ssea1

Pluripotency Marker Expression Analysis

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Primary antibodies used in this study for immunofluorescence and FACS are as follows: anti-Oct4 (Santa Cruz, C10), anti-Nanog (Cosmobio, RCA B000 2P-F), anti-thy1 (Ebiosciences, 53-2.1), anti-SSEA1 (Stem Cell Technologies, 60060PE) and NL493-conjugated anti-SSEA4 antibody (R&D Systems, SC023), anti-Tra1-60 (Stemgent, 09-0009).

Ozmadenci D., Féraud O., Markossian S., Kress E., Ducarouge B., Gibert B., Ge J., Durand I., Gadot N., Plateroti M., Bennaceur-Griscelli A., Scoazec J.Y., Gil J., Deng H., Bernet A., Mehlen P, & Lavial F. (2015). Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance. Nature Communications, 6, 7398.

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Characterization of Rat BMMSCs and Muse Cells

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Rat BMMSCs and Muse cells were incubated with the desired antibodies (e.g., anti‐CD29, anti‐CD90, anti‐RT1A, anti‐CD34, anti‐CD45, anti‐RT1B, anti‐SSEA‐3 [BioLegend, San Diego, USA], and anti‐SSEA‐1, [STEMCELL Technologies, Vancouver, BC, Canada]). Edu proliferation (RiboBio, Guangzhou, China) and Annexin V‐FITC/PI apoptosis (KeyGen BioTECH, Nanjing, China) detection kits were used to determine the cellular proliferation and apoptosis rates following the manufacturer's guidelines.

Sun D., Yang L., Cao H., Shen Z, & Song H. (2019). Study of the protective effect on damaged intestinal epithelial cells of rat multilineage‐differentiating stress‐enduring (Muse) cells. Cell Biology International, 44(2), 549-559.

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