Vicryl suture

A cathartic agent of polyethylene glycol was administered in the evening before the operation, and prophylactic single-dose injections of metronidazole 0.5 g intravenously were administered at the time of anesthesia induction. Routine examinations such as laboratory testing, liver and kidney function tests, and other biochemical measures were carried out, and no abnormalities were found.
All operations were performed under epidural anesthesia, and all patients were in the lithotomy position. The surgical procedure was based on the technique of Lin et al. [6 (link)] and was performed by a single experienced surgeon as follows: TST consists of two rows of 33 mm diameter titanium staples; the obturator was inserted into the anus for full dilatation; the three-window anoscope with the obturator was inserted into the anus again; the obturator was pulled out (Figure 1(a)); the mucosa was sutured using a 2/0 Vicryl suture (Ethicon, Cincinnati, OH, USA), at 3-4 cm above the dentate line; the TST was opened to the maximum; the 2/0 Vicryl suture was tied to the rod of the TST (Figure 1(b)); the stapler was strained and fired; and the TST was removed from the anal canal.
Details of operating time, length of hospital stay, patient satisfaction, and perioperative complications (occurring up to postoperative day 30) were collected, as described previously [11 (link)].

Mice received irradiation to the scalp, using methodology previously described.⁹, ¹⁷ A total of 30 Gy was delivered, fractionated into six 5 Gy doses on alternate days over a total of 12 days. Lead shielding was used to ensure only the scalp was irradiated. A 4‐week recovery period followed irradiation to allow for the chronic fibrotic effects of radiation to develop (Figure 1A). ASC‐enriched grafts were prepared by mixing 10 000 FACS‐sorted CD74+, CD74−, or US ASCs with 200 μL of fresh human lipoaspirate, based on previous titration studies evaluating effects of supplemental stromal cells.³⁵ Control mice received 200 μL of fresh human lipoaspirate not enriched with ASCs (n = 5 mice/group for a total n = 20). Fat was grafted subcutaneously, directly beneath the irradiated scalp skin. A subcutaneous tunnel was first created, and the grafts were delivered by using a 1 cc syringe and a 16‐gauge needle in a retrograde fashion. Fat was injected within 2 hours of original harvest. Injection sites were closed with 6‐0 Vicryl suture (Ethicon, Inc., Somerville, New Jersey). All animal studies were performed in accordance with Stanford University animal guidelines, under the Stanford Institutional Review Board approval (APLAC #31212; Figure S1).