Total proteins were extracted utilizing RIPA lysis buffer that contained phosphatase and protease inhibitors. Protein concentration was evaluated utilizing a bicinchoniniacid assay (Solarbio, Beijing). The later steps of the western blotting analysis were conducted using standard protocol. Antibody directed towards GAPDH was acquired from Novus Biologicals, while antibodies directed towards YAP, TEAD, Bcl-2, BAX, p-YAP (Ser127), CYR61, CTGF, AXL, Caspase9, Cleaved Caspase9, PARP, and Cleaved PARP were bought through Cell Signaling Technology (Danvers, MA).
Antibody directed towards gapdh
Manufactured by Novus Biologicals
Antibody directed towards GAPDH. This antibody recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, which is a key enzyme involved in the glycolytic pathway.
Automatically generated - may contain errors
4 protocols using antibody directed towards gapdh
1
Western Blot Analysis of Apoptosis Markers
2
Comprehensive Protein Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins were obtained utilizing RIPA lysis buffer that contained phosphatase and protease inhibitors. Protein concentration was evaluated utilizing a bicinchoniniacid assay (Solarbio, Beijing). The later steps of Western blotting were conducted using standard protocol. Antibody directed towards GAPDH was acquired from Novus Biologicals. Antibodies directed towards YAP, TEAD, Bcl-2, Bax, p-YAP (Ser127), CYR61, CTGF, AXL, Caspase9, Cleaved Caspase9, PARP, Cleaved PARP were bought through Cell Signaling Technology (Danvers, MA).
3
Comprehensive Protein Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins were obtained utilizing RIPA lysis buffer that contained phosphatase and protease inhibitors. Protein concentration was evaluated utilizing a bicinchoniniacid assay (Solarbio, Beijing). The later steps of Western blotting were conducted using standard protocol. Antibody directed towards GAPDH was acquired from Novus Biologicals. Antibodies directed towards YAP, TEAD, Bcl-2, Bax, p-YAP (Ser127), CYR61, CTGF, AXL, Caspase9, Cleaved Caspase9, PARP, Cleaved PARP were bought through Cell Signaling Technology (Danvers, MA).
4
Comprehensive Protein Analysis via Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins were extracted utilizing RIPA lysis buffer that contained phosphatase and protease inhibitors. Protein concentration was evaluated utilizing a bicinchoniniacid assay (Solarbio, Beijing). The laterter steps of the western blotting analysis were conducted using standard protocol. Antibody directed towards GAPDH was acquired from Novus Biologicals, while antibodies directed towards YAP, TEAD, Bcl-2, Bax, p-YAP (Ser127), CYR61, CTGF, AXL, Caspase9, Cleaved Caspase9, PARP, and Cleaved PARP were bought through Cell Signaling Technology (Danvers, MA).
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