Formalin‐fixed paraffin‐embedded (FFPE) 2‐mm human tonsil tissue cores were obtained from the Novopath Tissue Biobank (Royal Victoria Infirmary, Newcastle upon Tyne) and embedded into a three‐core TMA. TMA blocks were constructed manually using medical biopsy punches (PFM Medical, UK). Cores were selected using hematoxylin and eosin‐stained slides to guide suitable areas in the donor blocks. Cores were placed in a paraffin embedding mold, heated to 65°C and embedded in molten wax before cooling to set. Eight‐micrometer serial sections were cut using HM 325 Rotary Microtome (Fisher Scientific, USA) and mounted onto SuperFrost Plus™ Adhesion slides (Epredia, CAT#10149870).
Superfrost plus adhesion slides
Manufactured by Epredia
Sourced in United States
The SuperFrost Plus Adhesion Slides are laboratory slides designed to provide a secure surface for adhering and mounting tissue samples during histological and cytological procedures. They feature a positively charged surface that enhances the attachment of biological specimens.
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Lab products found in correlation
3 protocols using superfrost plus adhesion slides
1
Constructing Human Tonsil Tissue Microarray
2
Eye tissue preparation for cryosectioning
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Fish were euthanized as described above. Intracardial perfusion with phosphate buffered saline (NaHPO4.2H2O, 8mM; KH2PO4, 2 mM; NaCl, 0.15M; KCl, 3mM; pH 7.4) and 4% paraformaldehyde (PFA, in PBS, Merck-Aldrich) was executed, all as described (Bergmans et al., 2023c (link); Mariën et al., 2022 (link)). Eyes were collected and post-fixed overnight in 4% PFA, followed by three rinses in PBS. Next, eyes were incubated until saturation using an increasing series of sucrose concentrations (10, 20, 30% weight by volume in PBS) and embedded in 1.25% agarose and 30% sucrose in PBS for cryosectioning. For each eye, 10 μm sagittal cryosections were serially collected on eight SuperFrost Plus Adhesion Slides (Epredia) and stored at −20°C until further use (Bergmans et al., 2023c (link)).
3
Fixation and Sectioning of Fish Eyes
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Fish were euthanized using 0.1% tris-buffered tricaine and their body size (length; head to tail) was determined using callipers before performing intracardial perfusion with phosphate buffered saline (PBS, 0.01M, pH 7.4) and 4% paraformaldehyde (PFA, in PBS, Merck-Aldrich), all as described (Mariën et al. 2022) . Eyes were collected and post-fixed for 1 h in 4% PFA at room temperature (RT) and subsequently rinsed three times in PBS. Depending on the follow-up procedure, eyes were either incubated in increasing sucrose solutions (10, 20 & 30% in PBS, Merck) and embedded in 1.25% agarose and 30% sucrose in PBS for cryosectioning, or retinas were dissected and fixed for 1 h in 4% PFA at RT, rinsed three times in PBS and stored in storage buffer (0.4% NaN3 in PBS) until further use. 10 µm sagittal cryosections were cut using a NX70 cryostat (Epredia), serially collected on eight SuperFrost Plus Adhesion Slides (Epredia) per eye and stored at -20 °C until further use. In all cases retinal orientation (dorsal, ventral, nasal and temporal) was taken into account.
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