Ttaflox

For PEGASOS-cleared mouse: Following mice were purchased from the Jackson Lab with genotypes including Thy1-EGFP-M (JAX# 007788), Ai14 (JAX# 007908), tTA^flox (JAX# 008600), tetO-H2BGFP (JAX# 005104) and Wnt1-Cre2 (JAX# 022501). Tg(Cdh5-CreERT2) mice (Strain NO.T014691) were purchased from GemPharmatech (Nanjing, China). R26-mScarlett^flox reporter was generated by Hu Zhao lab in the Chinese Institute for Brain Research. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research. For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/ml. The solution was kept at −20 °C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.
For CUBIC-L/R cleared mouse: A fixed brain of 9-week-old female Thy1-YFP-H Tg mice (B6.Cg-Tg(Thy1-YFP)HJrs/J, The Jackson Laboratory, Identifier: 003782)^{63 (link)} was provided from National Institute for Physiological Sciences (Okazaki, Japan) under the material transfer agreement with Juntendo University. All animal experiments were approved by Juntendo University (1569-2022279 and 1372-2022211), and National Institute for Physiological Sciences (22A044).

Following mice were purchased from the Jackson Lab with genotypes including Thy1-EGFP-M (JAX# 007788), Ai14 (JAX# 007908), tTA^flox (JAX# 008600), tetO-H2BGFP (JAX# 005104) and Wnt1-Cre2 (JAX# 022501). Tg(Cdh5-CreERT2) mice (Strain NO.T014691）were purchased from GemPharmatech (Nanjing, China). R26-mScarlett ^flox reporter was generated by Hu Zhao lab in the Chinese Institute for Brain Research. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research. For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/ml. The solution was kept at −20°C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.