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Morgagni 268 electron

Manufactured by Thermo Fisher Scientific
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The Morgagni 268 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a LaB6 electron source, advanced optics, and a high-resolution digital camera system for capturing detailed micrographs.

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3 protocols using morgagni 268 electron

1

Recombinant WUPyV VP1 VLPs

Cited 2 times
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Macrophages were treated with VLPs composed of up to 360 monomers of WUPyV recombinant major capsid protein VP1 (MW 40 kDa). VLPs were produced in S. cerevisiae yeast expression system and purified by CsCl density gradient centrifugation as described previously [24 (link), 25 (link)]. The isolated VLPs were dissolved in PBS, dialysed, mixed with glycerol (1:1), filtered through a 0.2 µm pore size RC syringe filter and stored at -20 °C. VLP structure was verified by examination of the purified proteins using Morgagni-268 electron microscope (FEI, Inc., Hillsboro, OR, USA).
Lučiūnaitė A., Mašalaitė K., Plikusiene I., Maciulis V., Juciute S., Norkienė M, & Žvirblienė A. (2024). Structural properties of immune complexes formed by viral antigens and specific antibodies shape the inflammatory response of macrophages. Cell & Bioscience, 14, 53.
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2

Quantifying Microtubule Cross-Sectional Area

Cited 1 time
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For electron microscopy, ultrathin Epon plastic sections were prepared as described in (Kretzschmar et al., 1997 (link)). TEMs were imaged with a FEI Morgagni 268 electron microscope. For double-blind analyses, images were taken and the pictures numbered. The measurements of microtubule area are described in (Bolkan and Kretzschmar, 2014 (link)), selecting neurites which contained microtubules that were cross-sectioned. No more than 5 neurites per image and at least 3 images per animal were analyzed. Three animals per genotype were used. The cross-sectional area of microtubules was measured with Photoshop before the genotype was revealed. The D’Agostino and Pearson omnibus test was used to test for normal distribution, followed by ANOVA one-way analysis of variance and Dunnett’s post-tests.
Law A.D., Cassar M., Long D.M., Chow E.S., Giebultowicz J.M., Venkataramanan A., Strauss R, & Kretzschmar D. (2022). FTD-associated mutations in Tau result in a combination of dominant and recessive phenotypes. Neurobiology of disease, 170, 105770.
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3

Quantifying Microtubule Cross-Sectional Area

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For electron microscopy, ultrathin Epon plastic sections were prepared as described in (Kretzschmar et al., 1997 (link)). TEMs were imaged with a FEI Morgagni 268 electron microscope. For double-blind analyses, images were taken and the pictures numbered. The measurements of microtubule area are described in (Bolkan and Kretzschmar, 2014 (link)), selecting neurites which contained microtubules that were cross-sectioned. No more than 5 neurites per image and at least 3 images per animal were analyzed. Three animals per genotype were used. The cross-sectional area of microtubules was measured with Photoshop before the genotype was revealed. The D’Agostino and Pearson omnibus test was used to test for normal distribution, followed by ANOVA one-way analysis of variance and Dunnett’s post-tests.
Law A.D., Cassar M., Long D.M., Chow E.S., Giebultowicz J.M., Venkataramanan A., Strauss R, & Kretzschmar D. (2022). FTD-associated mutations in Tau result in a combination of dominant and recessive phenotypes. Neurobiology of disease, 170, 105770.
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