Vessels were incubated with various inhibitors for 30 minutes prior to conducting PE, ACh or SNP response curves. NO synthase inhibitor, NW-nitro-L-arginine methyl ester (L-NAME 100 μM; Sigma Aldrich), NADPH oxidase was inhibited by a non-specific inhibitor (Apocynin 100 μM) and a specific and novel inhibitor, 3-Benzyl-7-(2-benzoxazolyl)thio-1,2,3-triazolo(4,5-d)pyrimidine (VAS2870 10 μM; both from Sigma Aldrich St. Louis, MO). 1H-[2 (link),12 (link),55 (link)] Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ 10 μM; Sigma Aldrich St. Louis, MO), a guanylyl cyclase inhibitor, was also incubated with some vessels for 30 minutes prior to usage of PE, ACh or SNP. To protect against the potential deleterious effects of ROS, separate vessels, ex vivo, were incubated for 30 minutes with an antioxidant, N-acetyl cysteine (NAC 20 mM; Sigma Aldrich, St. Louis, MO), and the above myograph procedure was conducted as described above.
Oxadiazolo 4 3 a quinoxalin 1 one odq
Manufactured by Merck Group
Sourced in United States
Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) is a chemical compound used as a laboratory reagent. It functions as a selective inhibitor of soluble guanylate cyclase, an enzyme involved in the regulation of various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
L arginine, by Merck Group (2 mentions)
Glibenclamide, by Merck Group (2 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Apocynin, by Merck Group (1 mentions)
3 benzyl 7 2 ben zoxazolyl thio 1 2 3 triazolo 4 5 d pyrimidine vas2870, by Merck Group (1 mentions)
Nw nitro l arginine methyl ester l name, by Merck Group (1 mentions)
Hemin, by Merck Group (1 mentions)
5 hydroxydecanoate 5 hd, by Merck Group (1 mentions)
Corm 2, by Merck Group (1 mentions)
Pinacidil, by Merck Group (1 mentions)
Ly294002 ly, by Merck Group (1 mentions)
Diltiazem, by Merck Group (1 mentions)
Paxilline, by Merck Group (1 mentions)
Ng nitro l arginine methyl ester l name, by Merck Group (1 mentions)
Wortamannin, by Merck Group (1 mentions)
Corm 3, by Merck Group (1 mentions)
Carboxy ptio, by Merck Group (1 mentions)
Bay 60 7550, by Santa Cruz Biotechnology (1 mentions)
Kt5823, by Merck Group (1 mentions)
Paracetamol, by Merck Group (1 mentions)
5 protocols using oxadiazolo 4 3 a quinoxalin 1 one odq
1
Vascular Function Assessment with Inhibitors
2
Investigating Vasodilatory Pharmacology
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ANP was purchased from Bachem Holding AG (Bubendorf, Switzerland). CORM-2, CORM-3, Hemin, zinc porphyrin (ZnPP), pinacidil, 5-hydroxydecanoate (5-HD), diltiazem, NG-nitro-L-arginine methyl ester (L-NAME), paxilline, wortamannin, LY-294002 (LY), and 1H-[1 (link),2 (link),4 (link)] oxadiazolo [4,3-a] quinoxalin-1-one (ODQ) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Pharmacological Modulators of Cellular Signaling
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KT-5823, SNAP, carboxy-PTIO and 1H-[1 (link),2 (link),4 (link)]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were from Sigma (St. Louis, MO). LY-83583 and BAY-60-7550 were from Santa Cruz Biotechnology (Santa Cruz, CA). ODQ, LY-83583 and BAY-60-7550 were dissolved in dimethyl sulfoxide (DMSO). To ensure that the channel activity was not affected by DMSO, its final concentration in the bath was less than 0.1%.
4
Pharmacological Modulation of Nociception
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Paracetamol, β-caryophyllene oxide, methiothepin, glibenclamide, [1 ,2 (link),4 (link)] oxadiazolo [4,3-a]quinoxalin-1-one (ODQ), N(G)-nitro-l -arginine methyl ester hydrochloride (l -NAME), and l -arginine were obtained from Sigma-Aldrich (St. Louis, MO, USA), and naloxone was acquired from Pisa Laboratories (Mexico City, Mexico). All drugs were suspended in carboxymethylcellulose (0.1%) and prepared minutes before being given to the animals. β-caryophyllene oxide and Paracetamol were administered orally (0.1 mL/10 g of body weight). Naloxone, methiothepin, l -NAME, ODQ, l -arginine, and glibenclamide were injected intraperitoneally (0.1 mL/10 g of body weight). Formalin (2%) was prepared by diluting aqueous formaldehyde to 37% from J.T. Baker (Matsonford Rd. Radnor Township, PA, USA).
5
Vascular Reactivity Modulation Assay
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l -arginine, Nω-nitro-l -arginine (l -NOARG), 1H-[1 (link),2 (link),4 (link)]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), glibenclamide and acetic acid were purchased from Sigma Chemical Co. (St Louis, MO, USA). acetic acid, l -arginine, l -NOARG, glibenclamide and diazepam were dissolved in normal saline (0.9% NaCl). ODQ was dissolved in 5% DMSO.
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