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Quantikine elisa mouse cxcl12 sdf 1α

Manufactured by R&D Systems

The Quantikine ELISA Mouse CXCL12/SDF-1α is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure mouse CXCL12/SDF-1α levels in cell culture supernates, serum, and plasma.

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2 protocols using quantikine elisa mouse cxcl12 sdf 1α

1

SDF-1 Quantification in Plasma

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Plasma SDF-1 levels were run in duplicate according to manufacturer’s recommendations on a commercially available, quantitative technique of sandwich enzyme immune assay (Quantikine ELISA Mouse CXCL12/SDF-1α, R&D Systems, Minneapolis, MN). The data were standardized against the protein concentrations of each sample, which was determined by taking 1μl of each sample and using the Protein A280 spectrophotometric assay (NanoDrop ND1000 spectrophotometer, NanoDrop Technologies Inc, Wilmington, Delaware, USA).
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2

Serum CXCL12 Levels in High-Fat Diet

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The concentration of serum CXCL12 was measured at 5 and 26 weeks after feeding with HFD using R&D Systems Quantikine ELISA Mouse CXCL12/SDF-1α (#MCX120; R&D Systems).
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