Mononuclear cells were isolated for further experimentation as described.23 Briefly, livers were passed through a metal spleen screen and digested with 0.05% collagenase IV (Sigma‐Aldrich, St. Louis, MO), and intrahepatic mononuclear cells were purified by Histodenz density gradient centrifugation (Sigma‐Aldrich). Spleens were passed through a mesh spleen screen, and mononuclear cells were purified by Ficoll gradient.
Histodenz density gradient centrifugation
Manufactured by Merck Group
Histodenz is a density gradient centrifugation medium used for the separation and purification of cellular components, organelles, and macromolecules. It provides a stable, non-toxic environment for the isolation of biological samples during centrifugation.
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Lab products found in correlation
2 protocols using histodenz density gradient centrifugation
1
Isolation of Hepatic and Splenic Mononuclear Cells
2
Isolation of Mononuclear Cells from Liver and Spleen
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