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Unconjugated mouse and rat igg antibodies

Manufactured by Jackson ImmunoResearch
Sourced in United States

Unconjugated mouse and rat IgG antibodies are purified immunoglobulin G (IgG) antibodies derived from mouse and rat. They are available in different formats and can be used as secondary antibodies or for other applications that require an unconjugated antibody.

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2 protocols using unconjugated mouse and rat igg antibodies

1

Multicolor Flow Cytometry Antibodies

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Monoclonal antibodies against the following mouse antigens were used in this study: CD3ε (145.2C11), CD4 (RM4-5), CD8α (53–6.7), CD11b (M1/70), CD11c (N418), CD45 (30-F11), CD45R/B220 (RA3-6B2), CD90.2 (30-H12), CD127 (A7R34), CD170/Siglec-F (E50-2440), F4/80 (BM8), IL-10 (JESS-16E3), Ly6C (HK1.4), Ly6G (1A8). Antibodies were purchased from BD Biosciences (San Diego, USA), BioLegend (San Diego, USA), or ThermoFisher Scientific (Carlsbad, USA). Antibodies were conjugated to Pacific Blue, Brilliant Violet 421, V500, Brilliant Violet 510, Brilliant Violet 570, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 785, Brilliant Violet 786, FITC, Alexa Fluor 488, PerCP-Cy5.5, PerCP-eFluor 710, PE, PE-CF594, PE-Cy7, APC, Alexa Fluor 647, Alexa Fluor 700, APC-Cy7, or APC-Fire750. Details on the antibody used in this study are given in the S1 Table. Anti-mouse CD16/32 antibody (2.4G2) and Zombie UV Dead Cell Staining Kit were obtained from Tonbo Biosciences (San Diego, USA) and from BioLegend, respectively. Unconjugated mouse and rat IgG antibodies were purchased from Jackson ImmunoResearch (West Grove, USA).
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2

Comprehensive Mouse Immune Cell Profiling

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Monoclonal antibodies against the following mouse antigens were used in this study: CD3 (145.2C11), CD4 (RM4-5), CD8 (53-6.7), CD11b (M1/70), CD11c (N418), CD45 (30-F11), CD45R/B220 (RA3-6B2), CD90.2 (30-H12), CD127 (A7R34), CD170/Siglec-F (E50-2440), F4/80 (BM8), IL-10 (JESS-16E3), Ly6C (HK1.4), Ly6G (1A8). Antibodies were purchased from BD Biosciences (San Diego, USA), BioLegend (San Diego, USA), eBioscience (San Diego, USA) or ThermoFisher Scientific (Carlsbad, USA). Antibodies Fluor 700, APC-Cy7, APC-eFluor 780 or APC-Fire750. Anti-mouse CD16/32 antibody (2.4G2) and Zombie UV Dead Cell Staining Kit were obtained from Tonbo Biosciences (San Diego, USA) and from BioLegend, respectively. Unconjugated mouse and rat IgG antibodies were purchase from Jackson ImmunoResearch (West Grove, USA).
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