Airway responsiveness to intravenous methacholine (MCh) was measured based on the response of airway resistance using the FlexiVent ventilator system (SCIREQ, Montreal, QC, Canada), as previously described [22] (link). Maximal airway resistance was calculated in response to up to 25 mg/mL methacholine i.n. (n = 10 per group).
Flexivent ventilator system
Manufactured by SCIREQ
Sourced in Canada
The FlexiVent ventilator system is a laboratory equipment designed to measure respiratory mechanics in small animal models. It provides precise control and monitoring of ventilation parameters.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using flexivent ventilator system
1
Methacholine-Induced Airway Resistance
2
Airway Responsiveness in Allergic Mice
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Twenty-four hours after the final allergen challenge, mice were anesthetized with intraperitoneal ketamine/xylasine 20 mg/kg, a metal cannula was inserted in the trachea and mice were attached to a Flexivent ventilator system (Scireq, Montreal). Lung resistance were measured in response to increasing doses of methacholine up to 64 mg/mL [11] . Mice were then sacrificed and broncho-alveolar lavage (BAL) fluid obtained for differential cell counts and enzyme-linked immunosorbent assay (ELISA). Total and differential cell counts from BAL were performed using cytospin and Hematocolor staining. Lung function measurements were performed in a blinded fashion (without knowing what treatment the animal had received).
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