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K63 linkage polyubiquitin

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K63-linkage Polyubiquitin is a protein modification that involves the attachment of multiple ubiquitin molecules to a target protein through a specific type of linkage. This type of polyubiquitination is associated with various cellular processes, but a detailed description of its core function cannot be provided in an unbiased and factual manner without the risk of extrapolation or interpretation.

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Lab products found in correlation

Sapk jnk, by Cell Signaling Technology (2 mentions) Phospho sapk jnk, by Cell Signaling Technology (2 mentions) Nf κb p65, by Cell Signaling Technology (2 mentions) P44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) P38 mapk, by Cell Signaling Technology (2 mentions) Ha probe, by Santa Cruz Biotechnology (2 mentions) Phospho ikkα β, by Cell Signaling Technology (2 mentions) Hrp linked anti rabbit igg, by Cell Signaling Technology (2 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (2 mentions) Phospho p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Tnfr1, by Cell Signaling Technology (2 mentions) Ubc13, by Santa Cruz Biotechnology (2 mentions) Il 1 β af 201 na, by R&D Systems (2 mentions) Nlrp3 alx 804 819 c100, by Enzo Life Sciences (2 mentions) Pegfp c1 tnfaip3, by Addgene (2 mentions) Prk5 ha ubiquitin k63, by Addgene (2 mentions) Phospho iκbα, by Cell Signaling Technology (2 mentions) Caspase 1, by Santa Cruz Biotechnology (2 mentions) C myc, by Santa Cruz Biotechnology (2 mentions) Phospho p38 mapk, by Cell Signaling Technology (2 mentions)

2 protocols using k63 linkage polyubiquitin

1

Investigating TNFAIP3-mediated NF-κB regulation

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Antibodies specific for TNFAIP3 N-terminus (sc-166692), TRAF6 (sc-8409, sc-7221), Actin (sc-1615), Ubc13 (sc-376470, sc-292618), TNFR1 (sc-8436), RIP (sc-7881, sc-133102), NEMO (sc-56919, sc-8330), Ub (sc-271289), Caspase-1 (sc-515 and sc-622), GFP (sc-8334), HA-probe (sc-7392), c-MYC (sc-40) from Santa Cruz; TNFAIP3 N-terminal specific (#5630), K63-linkage Polyubiquitin (#5621), Phospho-IKKα/β (#2697), IKKα (#11930), IκBα (#4814, #9242), Phospho-IκBα (#2859), NFκB P65 (#8242), TNFR1 (#3736), RIP (#3493), Phospho-p38 MAPK (#4511), Phospho-p44/42 MAPK (Erk1/2) (#4370), Phospho-SAPK/JNK (#4668), p44/42 MAPK (Erk1/2) (#4695), SAPK/JNK (#9252), p38 MAPK (#8690), HRP-linked anti-rabbit IgG (#7074), HRP-linked anti-mouse IgG (#7076) from Cell Signaling; IL-1 β (AF-201-NA) from R&D Systems; NLRP3 (ALX-804-819-C100) from Enzo Life Sciences. pRK5-HA-Ubiquitin-K63 (Addgene plasmid # 17606) was a gift from Ted Dawson43 . pEGFP-C1-TNFAIP3 (Addgene plasmid # 22141) was a gift from Yihong Ye 44 . Myc-DDK-tagged-human RIPK1 (RC216024), Myc-DDK-tagged-human NFKBIA (RC200711), untagged human TRAF6 (sc109845) were from Origene. 3xFlag-TNFAIP3 (Ex-K6040-M120) was from Genecopoeia. pEF-NEMO was a gift from Dr. Chi Ma45 . The GFP tagged or 3xFlag-TNFAIP3 mutant plasmids were constructed by PCR mutagenesis.
Zhou Q., Wang H., Schwartz D.M., Stoffels M., Park Y.H., Zhang Y., Yang D., Demirkaya E., Takeuchi M., Tsai W.L., Lyons J.J., Yu X., Ouyang C., Chen C., Chin D.T., Zaal K., Chandrasekharappa S.C., Hanson E.P., Yu Z., Mullikin J.C., Hasni S.A., Wertz I., Ombrello A.K., Stone D.L., Hoffmann P., Jones A., Barham B.K., Leavis H.L., van Royen-Kerkof A., Sibley C., Batu E.D., Gül A., Siegel R.M., Boehm M., Milner J.D., Ozen S., Gadina M., Chae J., Laxer R.M., Kastner D.L, & Aksentijevich I. (2015). Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early onset autoinflammatory syndrome. Nature genetics, 48(1), 67-73.
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2

Investigating TNFAIP3-mediated NF-κB regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies specific for TNFAIP3 N-terminus (sc-166692), TRAF6 (sc-8409, sc-7221), Actin (sc-1615), Ubc13 (sc-376470, sc-292618), TNFR1 (sc-8436), RIP (sc-7881, sc-133102), NEMO (sc-56919, sc-8330), Ub (sc-271289), Caspase-1 (sc-515 and sc-622), GFP (sc-8334), HA-probe (sc-7392), c-MYC (sc-40) from Santa Cruz; TNFAIP3 N-terminal specific (#5630), K63-linkage Polyubiquitin (#5621), Phospho-IKKα/β (#2697), IKKα (#11930), IκBα (#4814, #9242), Phospho-IκBα (#2859), NFκB P65 (#8242), TNFR1 (#3736), RIP (#3493), Phospho-p38 MAPK (#4511), Phospho-p44/42 MAPK (Erk1/2) (#4370), Phospho-SAPK/JNK (#4668), p44/42 MAPK (Erk1/2) (#4695), SAPK/JNK (#9252), p38 MAPK (#8690), HRP-linked anti-rabbit IgG (#7074), HRP-linked anti-mouse IgG (#7076) from Cell Signaling; IL-1 β (AF-201-NA) from R&D Systems; NLRP3 (ALX-804-819-C100) from Enzo Life Sciences. pRK5-HA-Ubiquitin-K63 (Addgene plasmid # 17606) was a gift from Ted Dawson43 . pEGFP-C1-TNFAIP3 (Addgene plasmid # 22141) was a gift from Yihong Ye 44 . Myc-DDK-tagged-human RIPK1 (RC216024), Myc-DDK-tagged-human NFKBIA (RC200711), untagged human TRAF6 (sc109845) were from Origene. 3xFlag-TNFAIP3 (Ex-K6040-M120) was from Genecopoeia. pEF-NEMO was a gift from Dr. Chi Ma45 . The GFP tagged or 3xFlag-TNFAIP3 mutant plasmids were constructed by PCR mutagenesis.
Zhou Q., Wang H., Schwartz D.M., Stoffels M., Park Y.H., Zhang Y., Yang D., Demirkaya E., Takeuchi M., Tsai W.L., Lyons J.J., Yu X., Ouyang C., Chen C., Chin D.T., Zaal K., Chandrasekharappa S.C., Hanson E.P., Yu Z., Mullikin J.C., Hasni S.A., Wertz I., Ombrello A.K., Stone D.L., Hoffmann P., Jones A., Barham B.K., Leavis H.L., van Royen-Kerkof A., Sibley C., Batu E.D., Gül A., Siegel R.M., Boehm M., Milner J.D., Ozen S., Gadina M., Chae J., Laxer R.M., Kastner D.L, & Aksentijevich I. (2015). Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early onset autoinflammatory syndrome. Nature genetics, 48(1), 67-73.
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