L-02 cells (2 × 105 cells/well) were grown for 24 h, followed by treatment with SHK (0.25, 0.375 and 0.50 μM) for 4 h and exposure to APAP (10 mM) for 8 h. Then, GAH was assayed by a GSH test kit (Nanjing JianCheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.
Gsh test kit
Manufactured by Nanjing Jiancheng
Sourced in China
The GSH test kit is a laboratory equipment designed to measure the concentration of glutathione (GSH) in a given sample. It provides a reliable and quantitative assessment of GSH levels, which is a crucial antioxidant in biological systems. The test kit utilizes a standardized chemical reaction to determine the GSH content, allowing researchers and laboratories to accurately analyze and monitor GSH levels in their experiments or assays.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
H2o2 test kit, by Nanjing Jiancheng (1 mentions)
Bca protein assay kit, by Keygene (1 mentions)
Ros assay kit, by Nanjing Jiancheng (1 mentions)
Mda test kit, by Nanjing Jiancheng (1 mentions)
4 protocols using gsh test kit
1
APAP-Induced Hepatocyte Oxidative Stress
2
Measuring Intracellular GSH Levels
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To measure intracellular reduced glutathione (GSH) levels, RAW 264.7 cells were grown in 6-well plates (1 × 106 cells/well) for 24 h incubation, and then the cells were exposed to various concentrations of Lico A for 18 h and subsequently subjected to t-BHP (10 μM) for 3 h. According to the manufacturer's instructions, the level of intracellular of GSH was quantified using a commercially available GSH test kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The absorbance was measured at 405 nm using a microplate reader (Bio-Tek Instruments Inc.).
3
Antioxidant Enzyme Activity in Soybean Hairy Roots
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The activities of POD and SOD, H2O2 content, and GSH level in soybean hairy roots were measured as described previously [63 ,64 (link)]. In brief, total proteins were extracted using the vegetable protein extraction kit (KeyGene BioTECH, Nanjing, China) and quantified using a BCA protein assay kit (KeyGene BioTECH). The activities of POD and SOD, H2O2 content, and GSH level were measured using a peroxidase kit (Jiancheng BioTECH, Nanjing, China), superoxide dismutase kit (Keming BioTECH, Suzhou, China), H2O2 test kit (Jiancheng BioTECH, Nanjing, China), and GSH test kit (Jiancheng BioTECH, Nanjing, China) according to the instructions from the manufacturers and previously published methods [63 ,64 (link)]. All measurements were performed with four biological replications and three independent soybean hairy roots were used in each replication.
4
Oxidative Stress Markers in Brain Tissue
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The concentrations of glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA) and reactive oxygen species (ROS) in frozen brain tissue were measured by commercial GSH test kit (Cat# A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), total SOD test kit (Cat# A001-1-1, Nanjing Jiancheng Bioengineering Institute), MDA test kit (Cat# A003-1-1, Nanjing Jiancheng Bioengineering Institute) and ROS assay kit (Cat# E004-1-1, Nanjing Jiancheng Bioengineering Institute), respectively, according to the manufacturer’s instructions. The absorbance of wells was read at 412 nm (GSH), 532 nm (MDA) and 560 nm (SOD) using a microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). The fluorescence intensity of ROS was detected with the excitation and emission wavelengths at 500 nm and 525 nm, respectively, by Thermo Scientific Fluoroskan (Thermo Fisher Scientific).
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