The largest database of trusted experimental protocols

7 protocols using acetic acid

1

Analytical Techniques for Silymarin Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the chemicals used were of analytical grade: 0.9% normal saline (Albert David, India), silymarin (Sigma Chemicals, St Louis, MO, USA), chloroform (Rankem, India), ethanol (Changshu Yangyuan Chemical, China), petroleum ether (Fisher Scientific, India), acetone (Fisher Scientific), formaldehyde (Fisher Scientific), ethyl acetate (Himedia Chemicals, India), methanol (Fisher Scientific), diethyl ether (SD Fine Chemicals, India), acetic acid (SD Fine Chemicals), formic acid (Rankem). The equipment was: UV spectrophotometer (PharmaSpec UV-1700; Shimadzu, Japan), micropipette (Superfit, India), centrifuge (Spinnwell, India), digital balance (AUX220 Unibloc; Shimadzu), refrigerator (Intello Cool LG, India), and gel electrophoresis (Gel Doc XR +; Bio-Rad, USA).
+ Open protocol
+ Expand
2

Chemical Reagent Acquisition Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Diclofenac sodium, indomethacin, paracetamol, carrageenan, and yeast Brewer's were purchased from Sigma-Aldrich (St. Louis, USA). Acetic acid was purchased from S. D fine chemicals (Mumbai, Maharashtra, India), and Formalin was purchased from Qualigens fine Chemicals (Mumbai, Maharashtra, India).
+ Open protocol
+ Expand
3

HPLC Analysis of Efavirenz

Check if the same lab product or an alternative is used in the 5 most similar protocols
High-performance liquid chromatography (HPLC)-grade acetonitrile, methanol, and ammonium acetate were purchased from Merck (Mumbai, India). Acetic acid was obtained in analytical grade from SD Fine Chemicals Limited (Mumbai, India). Purified water was collected through the Milli-Q-Plus water purification system (Millipore, Milford, MA, USA). The reference substance of AMCOL was obtained from Sigma-Aldrich (St. Louis, MO, USA). Efavirenz was a gift sample from a local manufacturing company in Chennai, India. Polyvinylidene difluoride (PVDF) filter membranes (0.22 µm × 47 mm diametre) were purchased from Fisher Scientific Pvt. Ltd., (Mumbai, India).
+ Open protocol
+ Expand
4

Analytical Grade Chemical Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals used throughout this work were of analytical grade and the solvents were of spectroscopic grade. These included hydrochloric acid, toluene, ethyl acetate, acetic acid, sodium carbonate, formic acid, methanol, and chloroform (S. D. Fine Chemicals, Ahmedabad, Gujarat, India).
+ Open protocol
+ Expand
5

Synthesis of PVA-CS-AgNP Composite Films

Check if the same lab product or an alternative is used in the 5 most similar protocols
The chemicals used in the current study are as follows: Poly(vinyl alcohol), PVA (MW = 85–124 kDa, DH = 86 to 89%), and Acetic acid (purity ≥ 99.5%) were purchased from s.d.fine Chemicals Ltd., Mumbai, India. Chitosan, CS (MW = 200 kDa; N-deacetylation degree 75–85%) and silver nanopowder (purity ≥ 99.5%, particle size < 150 nm) were purchased from Sigma-Aldrich Chemicals, St. Louis, MO, USA. Rectified ethanol (95.6 vol.%) from Godavari Biorefineries, Karnataka, India. Chemicals purchased were used without further purifications as they are reagent-grade chemicals.
+ Open protocol
+ Expand
6

Kenaf Fiber and Cowhide Waste Utilization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kenaf fiber was extracted from the kenaf tree (cultivated traditionally by farmers for making ropes to tie piles of rice after harvest for storage) collected in Issakaha village, from the Korhogo region, Côte d’Ivoire (Ivory Coast). All the plant experiments were in compliance with relevant institutional, national and international guidelines and legislation. Freshly flayed hides was purchased from the local slaughterhouse, Chennai, India. Raw cowhide trimming wastes were collected from the leather processing unit at CSIR-Central Leather Research Institute, Chennai, India. Hide powder was prepared from raw cowhide trimming wastes and were treated using the conventional process to remove the unwanted hair and flesh based on our previous report37 (link). All chemicals were of analytical grade and were used without any further purification. Chemicals used were sodium hydroxide (SD Fine Chem Limited, 98%), sodium chlorite (LOBA Chemie, 80%), sodium sulfite anhydrous (EMPARTA, 98%), acetic acid (SRL, 99.9%), acetone (SD Fine Chem Limited), citric acid, titanium (IV) isopropoxide (Sigma Aldrich, 97%), iron (II) sulphate (SD Fine Chem Limited, 99%), iron (III) sulphate (SD Fine Chem Limited, 75%), ammonia (Rankem), isopropanol (Qaligens, 70%), ethanol (Hayman, 100%) and nitric acid (SD Fine Chem Limited, 69–72%).
+ Open protocol
+ Expand
7

Chitosan-Minocycline Nanoparticle Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chitosan (low molecular weight ranging between 6000-7000 Daltons) was purchased from Sigma Aldrich, New Delhi, India. Minocycline hydrochloride was obtained as a gift sample from Ranbaxy Pharmaceuticals, Ponta Sahib, Haryana, India. Glutaraldehyde (25% v/v aqueous solution) and Sodium tripolyphosphate (TPP) were purchased from Loba Chem, Mumbai, India. Liquid paraffin (heavy : light; 1 : 1), 0.1 M phosphate buffer saline (pH 6.8), acetic acid, acetone, 0.9% NaCl solution, Span 80, and Tween 80 were purchased from S.D. Fine Chemicals, Mumbai, India. Double distilled, deionized water produced in our lab was used throughout the study. All chemicals and solvents were of analytical grade and were used without further purification. Absorbance spectroscopy was performed on Shimadzu UV-Visible 1601PC spectrophotometer (Shimadzu Corporation, Maryland, USA), Differential Scanning Calorimetry was performed on Differential Scanning Calorimeter (DSC 8000, Perkin Elmer, Sao Paulo, Brazil), Surface morphology was studied using Philips SEM 500 scanning electron microscope, In vitro release study was performed on the Water Bath Shaker (Everest Analyticals, Mumbai, India). Statistical designing was performed using Stat-Ease Design-Expert version 11.0 (Minneapolis, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!