Dopamine and serotonin levels were measured in each supernatant by HPLC with electrochemical detection (Waters, Bangalore, India). Samples were injected using a Rheodyne 7725i injector valve with a 20 μL injection loop. The mobile phase consisting of 7% acetonitrile, 3% methanoland 90% 20 mM citric acid, 10 mM monobasic phosphate sodium, 3.25 mM octanesulfonic acid, 3 mM heptanesulfonic acid, 0.1 mM EDTA, 2 mM KCl, 6 mL/L o-phosphoric acid and 2 mL/L diethylamine (pH 3) was pumped at 0.3 mL min−1 with a Gold 118 system (Beckman, Fullerton, CA). Signals were recorded and quantified with a Beckman Gold 118integrator. Amounts of dopamine and serotonin were calculated by comparing peak levels from the supernatant samples with those of externalstandards. Monoamine levels were calculated as nM/mg weight tissue.
Gold 118 system
Manufactured by Beckman Coulter
Sourced in United States
The Gold 118 system is a piece of laboratory equipment manufactured by Beckman Coulter. It is designed to perform specific functions within a laboratory setting. The core function of the Gold 118 system is to [insert concise, factual description of the system's primary purpose or capabilities, without any interpretation or extrapolation].
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Lab products found in correlation
2 protocols using gold 118 system
1
Quantification of Monoamine Neurotransmitters
2
Quantifying Striatal Dopamine by HPLC
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Striatal DA levels were determined by the high-performance liquid chromatography (HPLC) with an electrochemical detector by the method of Muralikrishnan and Mohanakumar.[33 (link)] Briefly, the procured ST region was immediately sonicated in 0.1 M perchloric acid (ice-cold condition) containing 0.01% ethylenediaminetetraacetic acid (EDTA). Samples were centrifuged (10,000 g for 10 min) and the supernatant was collected and injected (10 μl) into the HPLC system using a rheodyne 7725i injector valve with a 20 μl injection loop. The mobile phase consisting of 7% acetonitrile, 3% methanol, and 90% 20 mM citric acid, 10 mM monobasic phosphate sodium, 3.25 mM octane sulfonic acid, 0.1 mM EDTA, 3 mM heptane sulfonic acid, 2 mM KCL, 6 mL/L o-phosphoric acid, and 2 mL/L diethylamine (pH 3) was allowed to flow at a rate of 0.3 mL/min with a Gold 118 system (Beckman, Fullerton, CA, USA). Results were expressed in ng/mg weight of brain tissue.
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