Human tnf α elisa kit

A human TNF-α ELISA Kit (ABclonal, cat no: RK0030) and a human IL-6 ELISA Kit (ABclonal, cat no: RK00004) were used to detect the TNF-α IL-6 cytokine levels in a medium with HK-2 cells transfected and treated with drug-containing serum.

The cell supernatant was collected and centrifuged at 1000× g for 10 min at 4°C to remove particulates. The concentration of TNF-α in the cell supernatant was determined by human TNF-α ELISA kit (Abclonal Technology, RK00030) according to the manufacturer’s instructions. For mouse placenta tissue, the placentas were cut into pieces (~1 mm³) and homogenized in pre-cooling HBSS solution (Sigma) on ice. The tissue suspension was then sonicated with an ultrasonic cell disrupter till the solution is clarified, and centrifuged at 10,000× g for 10 min at 4°C. Collect the supernatant for subsequent experiments. The concentration of TNF-α was determined by mouse TNF-α ELISA kit (Abclonal Technology, RK00027) according to the manufacturer’s instructions.