Goat anti rabbit igg antibody
The Goat anti-rabbit IgG antibody is a laboratory reagent used to detect and quantify rabbit immunoglobulin G (IgG) in various immunoassays and research applications. It is a secondary antibody produced in goats that specifically binds to rabbit IgG molecules, allowing for the identification and measurement of rabbit IgG in biological samples.
Lab products found in correlation
24 protocols using goat anti rabbit igg antibody
Dexamethasone Induces Mitochondrial Dysfunction
Immunoblotting of Per a 2 Protein
Protein Expression Analysis in Cancer Cells
Autophagy Regulation in DMEM/F12 Cell Culture
Quantitative Immunoblot Analysis
Steroid Hormone Measurement Protocol
E2 levels in plasma were also measured using the EIA methods as described by Isobe [18 (link)], with some modifications. Briefly, 75
µl of extracted estradiol-17β standard or the sample and 25 µl of the 1:10,000 diluted estradiol-17β antibody (ab215528, Abcam, Cambridge, UK) were
applied to the wells of plates that had been previously coated with goat anti-rabbit IgG antibody (Millipore; Merck & Co., Inc., Kenilworth, NJ, USA). After 1 hr incubation, 25
µl of 1:2,500 diluted estradiol-6-CMO-HRP antigen (East Coast Bio, Inc., North Berwick, ME, USA) was added to the wells. The plate was incubated for another hour and then
washed six times. The substrate solution was applied to the wells, and then, they were incubated for 30 min at room temperature. The reaction was stopped by the addition of 1 M
H3PO4. The optical density was calculated at 450 nm. Sensitivity was 1.6 pg/ml for E2 and 0.5
ng/ml for P4. The intra-assay and inter-assay CVs were 11.4% and 6.7% for E2 and 8.6% and 3.2% for P4, respectively.
Western Blot and qRT-PCR Reagents
Antibody Characterization for Cell Analysis
Autophagy-related Proteins Modulation
Western Blot Analysis of Protein Targets
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