1 2 dioleoyl 3 trimethylammonium propane chloride dotap

Manufactured by Lipoid

Sourced in Germany

1,2-dioleoyl-3-trimethylammonium-propane chloride (DOTAP) is a cationic lipid commonly used in biotechnology and pharmaceutical research. It acts as a transfection agent, facilitating the delivery of nucleic acids, such as DNA and RNA, into cells. DOTAP's amphiphilic structure allows it to form liposomes and lipoplexes, which can encapsulate and protect genetic material. The positive charge of DOTAP enables it to interact with the negatively charged phosphate groups of nucleic acids, enabling their cellular uptake.

Automatically generated - may contain errors

Lab products found in correlation

Tissue culture plasticware, by BD (1 mentions)

Spelling variants of this product

DOTAP (9 citations)

2 protocols using 1 2 dioleoyl 3 trimethylammonium propane chloride dotap

Antioxidant Activity Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium chloride (NaCl), calcium chloride (CaCl₂), sodium phosphate dibasic (Na₂HPO₄), cerium (III) nitrate, sodium citrate tribasic dihydrate (C₆H₅Na₃O₇•2H₂O), ammonia solution (28–30%), and the SOD assay kit (cat. n. 19,160) were obtained from Merck Life Science S.r.l. (Milan, Italy). 1,2-dioleoyl-3-trimethylammonium-propane chloride (DOTAP) was kindly donated by Lipoid GmbH (Ludwigshafen, Germany) while N-palmitoyl-sphingosine-1-{succinyl[methoxy(polyethylene glycol)2000]} (Cer₁₆-PEG₂₀₀₀) was purchased from Avanti Polar Lipids (Alabaster, USA). The 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate solution (cat. n. N301) and the Stop solution (cat. n. N600) were purchased from ThermoFisher Scientific (Rodano, Italy) while regenerated cellulose (RC) syringe filters with a 0.2 μm pore size were purchased from Exacta + Optech Labcenter SpA (San Prospero, Italy).

Nele V., Tedeschi V., Campani V., Ciancio R., Angelillo A., Graziano S.F., De Rosa G, & Secondo A. (2023). Cerium-Doped Self-Assembling Nanoparticles as a Novel Anti-Oxidant Delivery System Preserving Mitochondrial Function in Cortical Neurons Exposed to Ischemia-like Conditions. Antioxidants, 12(2), 358.

+ Open protocol

+ Expand

Nanoparticle-Mediated Cytotoxicity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless otherwise stated, all chemicals were from Sigma-Aldrich (Saint Louis, MO). 1,2-dioleoyl-3-trimethylammonium-propane chloride (DOTAP) and 1,2-diacyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene-glycol)-2000] (DSPE-PEG2000) were obtained from Lipoid GmbH (Cam, Switzerland). RPMI 1640 were purchased from FlowLaboratories (Milan, Italy). Tissue culture plasticware was from Becton Dickinson (Lincoln Park, NJ). ZOL was provided by Novartis (Novartis, Basilea, Svizzera). TMZ (Sigma Life Science) and FTM were provided by Prof. C. Leonetti (Experimental Chemotherapy Laboratory, Regina Elena National Cancer Institute IRCCS - Rome). NPs were provided by Prof. G. De Rosa (Department of Pharmaceutical Chemistry, University of Naples,‘‘Federico II’’).

Porru M., Zappavigna S., Salzano G., Luce A., Stoppacciaro A., Balestrieri M.L., Artuso S., Lusa S., De Rosa G., Leonetti C, & Caraglia M. (2014). Medical treatment of orthotopic glioblastoma with transferrin-conjugated nanoparticles encapsulating zoledronic acid. Oncotarget, 5(21), 10446-10459.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!