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Anti cd25 alexa 700

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Anti-CD25 Alexa 700 is a fluorophore-conjugated antibody that specifically binds to CD25, also known as the interleukin-2 receptor alpha chain. This product can be used for the detection and analysis of CD25-expressing cells in flow cytometry applications.

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Lab products found in correlation

Anti cd3 pe cy7, by BioLegend (3 mentions) Fortessa flow cytometer, by BD (3 mentions) Anti cd3 alexa700, by BioLegend (3 mentions) Cd8 apc, by BioLegend (2 mentions) Cd4 pe cy7, by BioLegend (2 mentions) Cd44 pe, by BioLegend (2 mentions) Cd4 pacific blue, by BioLegend (2 mentions) Anti foxp3 efluor 450 clone fjk 16 s, by Thermo Fisher Scientific (2 mentions) Anti cd36 apc, by BioLegend (2 mentions) Slc27a1, by Merck Group (2 mentions) Slc27a4, by Abcam (2 mentions) Goat anti rabbit igg alexa 647, by Jackson ImmunoResearch (2 mentions) Anti cd62l apc cy7, by BioLegend (1 mentions) Anti cd4 pacific blue, by BioLegend (1 mentions) Anti cd4 pe cy7, by BioLegend (1 mentions) Anti cd44 pe, by BioLegend (1 mentions) Anti cd8 apc, by BioLegend (1 mentions) Countbright beads, by Thermo Fisher Scientific (1 mentions) Facsdiva software, by BD (1 mentions) Anti cd4 fitc, by BioLegend (1 mentions)

Spelling variants of this product

CD25 Alexa 700 (3 citations)

4 protocols using anti cd25 alexa 700

1

Comprehensive Immune Profiling by Flow Cytometry

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For in-vitro studies, the following flow cytometry panel was used in conjunction with CTV and viability dye: anti-CD3 Alexa700, anti-CD4 PE-Cy7, anti-CD8 APC, and anti-CD44 PE, all at a 1:200 dilution (Biolegend). For in-vivo and ex-vivo studies, the following flow cytometry panel was used: anti-CD3 PE-Cy7, anti-CD4 Pacific Blue, anti-CD8 APC, anti-CD44 PE, anti-CD62L APC-Cy7, and anti-CD25 Alexa 700, all at a 1:200 dilution, except anti-CD25 at a 1:50 dilution (Biolegend). Endogenous Foxp3 expression was detected either via eGFP of eYFP fluorescence, which is compatible with all panels but not when used with BODIPY-FL dye (493/503) or 2-NBDG (465/540). In those assays, Foxp3-based uptake of FA or Glucose was not performed, but all other cellular subsets described were tested. All acquisition was performed using a BD Fortessa flow cytometer (BD, MD, USA) at the Northwestern University Comprehensive Flow Cytometry core.
Muroski M.E., Miska J., Chang A.L., Zhang P., Rashidi A., Moore H., Lopez-Rosas A., Han Y, & Lesniak M.S. (2017). Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate. Scientific Reports, 7, 5790.
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2

Flow Cytometry Immunophenotyping Protocol

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For in-vitro studies the following flow cytometry panel was used in conjunction with CTV and viability dye: anti-CD3 Alexa700, CD4 PE-Cy7, CD8 APC, CD44 PE all at a 1:200 dilution purchased from Biolegend. For in-vivo and ex-vivo studies, the following flow cytometry panel was used: anti-CD3 PE-Cy7, CD4 Pacific Blue, CD8 APC, CD44 PE, and anti-CD25 Alexa 700 all at a 1:200 dilution (except anti-CD25 at a 1:50 dilution) and were purchased from Biolegend. Endogenous Foxp3 expression was detected via eYFP fluorescence (or re-stained with anti-Foxp3 Efluor-450 clone FJK-16 s for any overnight staining (Fisher)), all acquisition was performed using a BD Fortessa flow cytometer. For surface FA transporter measurement, anti-CD36-APC (Biolegend), SLC27A1 (sigma), and SLC27A4 was purchased from Abcam (Cambridge, UK), followed by secondary goat anti-rabbit IgG Alexa-647 (Jackson Immunoresearch, West Grove, PA). Proliferation was analyzed using both FACS DIVA software as well as FlowJo software to determine expansion indexes.
Miska J., Lee-Chang C., Rashidi A., Muroski M.E., Chang A.L., Lopez-Rosas A., Zhang P., Panek W.K., Cordero A., Han Y., Ahmed A.U., Chandel N.S, & Lesniak M.S. (2019). HIF-1α Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma. Cell reports, 27(1), 226-237.e4.
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3

Flow Cytometry Immunophenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in-vitro studies the following flow cytometry panel was used in conjunction with CTV and viability dye: anti-CD3 Alexa700, CD4 PE-Cy7, CD8 APC, CD44 PE all at a 1:200 dilution purchased from Biolegend. For in-vivo and ex-vivo studies, the following flow cytometry panel was used: anti-CD3 PE-Cy7, CD4 Pacific Blue, CD8 APC, CD44 PE, and anti-CD25 Alexa 700 all at a 1:200 dilution (except anti-CD25 at a 1:50 dilution) and were purchased from Biolegend. Endogenous Foxp3 expression was detected via eYFP fluorescence (or re-stained with anti-Foxp3 Efluor-450 clone FJK-16 s for any overnight staining (Fisher)), all acquisition was performed using a BD Fortessa flow cytometer. For surface FA transporter measurement, anti-CD36-APC (Biolegend), SLC27A1 (sigma), and SLC27A4 was purchased from Abcam (Cambridge, UK), followed by secondary goat anti-rabbit IgG Alexa-647 (Jackson Immunoresearch, West Grove, PA). Proliferation was analyzed using both FACS DIVA software as well as FlowJo software to determine expansion indexes.
Miska J., Lee-Chang C., Rashidi A., Muroski M.E., Chang A.L., Lopez-Rosas A., Zhang P., Panek W.K., Cordero A., Han Y., Ahmed A.U., Chandel N.S, & Lesniak M.S. (2019). HIF-1α Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma. Cell reports, 27(1), 226-237.e4.
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4

Comprehensive Thymocyte Immunophenotyping

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Anti-CD4–Horizon V450, anti-CD4–FITC, anti-CD8–FITC, anti-CD8–Alexa647, anti-CD24–PE, anti-CD25–Alexa700, anti-CD25–APC-Cy7, anti-CD44–Horizon V450, anti-CD44–PE-Cy7, anti-CD45.1–Pacific Blue, anti-CD45.2–PE-Cy5, anti-CD117–PE-Cy7, anti-Sca-1–Alexa700, anti-TCRβ–PE, anti-TCRβ–PE-Cy5, and anti-Ter119–FITC were purchased from BioLegend (San Diego, CA) or BD Biosciences (San Jose, CA). Lineage markers were identified using biotinylated anti-Ter119, anti-CD11b, anti-Gr-1, anti-CD3ε, and anti-B220 followed by streptavidin-APC-Cy7 (Biolegend). Cell labeling was performed in PBS containing 2 % FCS. To quantify the number of thymocytes and mast cells, CountBright™ beads (Life Technologies, Grand Island, NY) were added.
Flow cytometry studies were performed using a BD LSR II (BD Immunocytometry Systems, San Jose, CA). Data were analyzed using BD FACSDiva software (BD Biosciences, San Jose, CA). Thymocytes were gated on the live lymphocyte gate, and doublet discrimination was performed. Thymocytes were gated on the Ter119−CD45+ population.
Xiong J., Parker B.L, & Yankee T.M. (2014). The combined loss of Gads and CD127 reveals a novel function of Gads prior to TCRβ expression. Immunologic research, 60(1), 77-84.
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