We examined approximately 650 specimens that were collected in South Korea from 2003 to 2017. All were deposited in the Korean Lichen Research Institute, Sunchon National University (KoLRI). The morphological characteristics and chemical spot tests were conducted under a dissecting microscope (Nikon SMZ 745T, Tokyo, Japan) and Olympus BX 50 microscope, and photographs were taken under a Carl Zeiss MicroImaging microscope with an Axio Cam ERc 5s imaging system (Carl Zeiss MicroImaging, GmbH 37081, Göttingen, Germany). All measurements based on the sections from the thalli and apothecia were recorded in water. The ascospore dimensions ranged from 20–80 spores from a single apothecium per specimen. The secondary metabolites were studied according to the spot tests in a solution (K = 10% aqueous KOH solution; C = saturated aqueous Ca(OCl)2; KC = 10% aqueous KOH solution followed by saturated aqueous Ca(OCl)2; P = 5% alcoholic p-phenylenediamine solution) and thin layer chromatography (TLC) in solvent C [48 ,49 ], and zeorine and atranorin were selected as controls.
Smz 745 t
Manufactured by Olympus
Sourced in Japan
The SMZ 745 T is a stereo microscope designed for laboratory use. It provides a magnification range from 0.67x to 5.6x, allowing for detailed observation and analysis of samples. The microscope features a trinocular observation tube, enabling the connection of a camera for documentation and image capture.
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Lab products found in correlation
3 protocols using smz 745 t
1
Lichen Specimen Analysis in South Korea
2
Morphological and Anatomical Characterization of Lichens
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The morphological and anatomical characteristics of the specimens were examined under a dissecting microscope (Nikon SMZ 745 T; Tokyo, Japan) and Olympus BX 50 microscope (Olympus, Tokyo, Japan). Photos were taken under HD-Measure LTHS-300 (Leetech Co., Seoul, South Korea) microscope and Carl Zeiss MicroImaging with Axio Cam ERc 5 s imaging system (Carl Zeiss MicroImaging, GmbH, Gottingen, Germany). All measurements based on the sections from thallus and apothecia were made in the water. Minimum-maximum values and the maximum value are recorded for granule, asci, and ascospores size. The number (N) of granules, asci, and spores were measured, and mean values (in italics), standard deviations (S.D.), quotient of length and width (Q), and average quotient [Q (m)] were calculated. The terminology to describe leprose lichens follows Lendemer [13 ].
3
Lichen Specimen Collection and Analysis
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Three specimens in this study were collected from South Korea and deposited at Sunchon National University, Korean Lichen Research Institute (KoLRI). Morphological and anatomical characters were recorded under a dissecting microscope (Nikon SMZ 745 T, Tokyo, Japan) and an Olympus BX 50 microscope (Olympus, Tokyo, Japan), and images were captured using an HD-Measure LTHS-300 (Leetech Co., Seoul, South Korea) microscope and a Carl Zeiss MicroImaging with Axio Cam ERc 5 s imaging system (Carl Zeiss MicroImaging, GmbH 37081, Gottingen, Germany). The secondary metabolites were studied using the spot test (K = 10% aqueous KOH solution; C = saturated aqueous Ca(ClO)2; KC = 10% aqueous KOH solution followed by saturated aqueous Ca(ClO)2; PD = 5% alcoholic p-phenylenediamine solution) and thin-layer chromatography (TLC) in solvent C [15 ,16 ]. UV tests were performed using a UV Chamber (CE07 21470) under long (366 nm) wavelength.
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