Il 6 elisa kit

Manufactured by BioLegend

Sourced in United States

The IL-6 ELISA kit is a laboratory instrument used to measure the concentration of interleukin-6 (IL-6) in biological samples. IL-6 is a cytokine that plays a crucial role in immune response and inflammation. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to quantify the levels of IL-6 present in the sample.

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Lab products found in correlation

Tnf α, by BioLegend (5 mentions) Il 1β, by BioLegend (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Anti actin, by Proteintech (2 mentions) Nf κb signal pathway kit, by Cell Signaling Technology (2 mentions) Anti bcl 2, by Proteintech (2 mentions) Anti bax, by Proteintech (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Tnf α elisa kit, by BioLegend (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Microplate reader, by Thermo Fisher Scientific (2 mentions) Nuclear extraction kit, by Active Motif (2 mentions) Transam elisa kit, by Active Motif (2 mentions) Rmmfg e8, by R&D Systems (1 mentions) Anti histone h3, by Proteintech (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Lipopolysaccharide escherichia coli serotype 0111 b4, by Merck Group (1 mentions) Anti mouse ly 6g gr 1 fitc, by Thermo Fisher Scientific (1 mentions) Tamoxifen, by Merck Group (1 mentions)

30 protocols using il 6 elisa kit

Evaluating Inflammatory Signaling and Renal Function

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TNF-α, IL-1β, and IL-6 ELISA Kits were purchased from Biolegend (San Diego, CA,
USA). rmMFG-E8 was purchased from R&D Systems (Minneapolis, MN, USA).
Antibodies used for western blot were as follows: anti-Bcl-2 (Proteintech,
Rosemont, IL, USA), anti-Bax (Proteintech, Rosemont, IL, USA), anti-ß-actin
(Proteintech, Rosemont, IL, USA), and anti-histone H3 (Proteintech, Rosemont,
IL, USA). A NF-κB signal pathway kit was used to determine NF-κB pathway
activation (Cell Signaling Technology, Beverly, MA, USA). Blood urea nitrogen
(BUN) and serum creatinine (Cre) detection kits were purchased from the
Institute of Jiancheng Bioengineering (Nanjing, China). All other reagents were
of analytical grade.

Zhao Y., Wang Q, & Zang B. (2019). Milk fat globule-epidermal growth factor 8 (MFG-E8) attenuates sepsis-induced acute kidney injury by inhibiting NF-κB signaling pathway. Acta Cirúrgica Brasileira, 34(2), e201900209.

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Tamoxifen Modulates Inflammation and Kidney Injury

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Tamoxifen and lipopolysaccharide (Escherichia coli serotype 0111:B4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). TNF-α and IL-6 ELISA kits were obtained from Biolegend (San Diego, CA, USA). Blood urea nitrogen (BUN) assay kit was obtained from Arbor Assays (Ann Arbor, Michigan, USA). Kidney injury molecule-1 (KIM-1) assay kit was purchased from R&D (Minneapolis, MN, USA). Anti-Mouse Ly-6G (Gr-1)-FITC was purchased from eBioscience (San Diego, CA, USA). Goat anti-mouse ICAM-1 and VCAM-1 antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA). Phospho-STAT3 (Thr705), Stat3 (124H6), phospho-p44/42MAPK (ERK1/2) (Thr202/Tyr204), p44/42MAPK (ERK1/2), phosphorylated IκBα, IκBα, and β-actin antibodies were obtained from Cell Signal Technology (Boston, MA, USA).

Gao R., Chen J., Hu Y., Li Z., Wang S., Shetty S, & Fu J. (2014). Sirt1 Deletion Leads to Enhanced Inflammation and Aggravates Endotoxin-Induced Acute Kidney Injury. PLoS ONE, 9(6), e98909.

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Cytokine Expression Profiling in Mice

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Mouse IL-17, IL-22, IL-10, IFN-γ, TNF-α, and IL-6 ELISA kits were purchased from Biolegend. The detection for each cytokine was performed following manufacturer’s instructions.

Sun M., He C., Chen L., Yang W., Wu W., Chen F., Cao A.T., Yao S., Dann S.M., Dhar T.G., Salter-Cid L., Zhao Q., Liu Z, & Cong Y. (2018). RORγt represses Th17 cell IL-10 production to maintain their pathogenicity in inducing intestinal inflammation. Journal of immunology (Baltimore, Md. : 1950), 202(1), 79-92.

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Quantifying Immune Modulators and Histone Modifications

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The supernatants of cultured and ablated KPC and S2013 were analyzed using murine and human MIF ELISA kits (R&D Systems). Supernatant from in vivo trained ex vivo stimulated CD11b+ cells were analyzed using murine TNF-α and interleukin (IL)-6 ELISA kits (BioLegend). To detect histone modification, histones were extracted from CD11b+ cells, which were positively selected from PBS, β-glucan, IRE, and β-glucan plus IRE treated KPC tumors using the Histone Extraction Kit (Active Motif, Catalong # 40028). Histone modifications were quantified using EpiQuik Total Histone H3, Acetyl histone H3K27, Tri-Methyl histone H3K4, and Tri-methyl histone H3K27 Quantification Kits (Colorimetric). Supernatants from in vitro trained human CD14+ cells were analyzed using human TNF-α ELISA kits (BioLegend). All assays were performed using the provided manufacturer instructions and all conditions were performed in duplicates.

Woeste M.R., Shrestha R., Geller A.E., Li S., Montoya-Durango D., Ding C., Hu X., Li H., Puckett A., Mitchell R.A., Hayat T., Tan M., Li Y., McMasters K.M., Martin R.C, & Yan J. (2023). Irreversible electroporation augments β-glucan induced trained innate immunity for the treatment of pancreatic ductal adenocarcinoma. Journal for Immunotherapy of Cancer, 11(4), e006221.

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GFP-NDV Infection and Innate Immune Response

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GFP-NDV was generously provided by Dr. Christopher Basler with permission from Dr. Peter Palese [57 (link)]. GFP-NDV (2X10⁴ fluorescent focus forming units (FFU)) was added to RAW264.7 and U937 cells and mouse macrophages in serum-free medium, and then incubated at 37ºC for 1.5 h, before complete medium was replaced. For TLR7 challenge, cells were incubated with Gardiquimod or HIV1 ssRNA40, with indicated concentrations and time-points. Human and mouse IFNα production was measured by ELISA with human and mouse VeriKine IFNα ELISA kits (PBL Assay Science), respectively. IL-6 ELISA kits were from BioLegend, Inc.

Wang L., Zhao J., Ren J., Hall K.H., Moorman J.P., Yao Z.Q, & Ning S. (2016). Protein phosphatase 1 abrogates IRF7-mediated type I IFN response in antiviral immunity. European journal of immunology, 46(10), 2409-2419.

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Cytokine Profiling of Macrophage Response

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The concentrations of cytokines in supernatants collected from LA-stimulated human and murine macrophages were measured using human and murine TNF-α, IL-1β, and IL-6 ELISA kits according to the manufacturer’s instructions (BioLegend). In all in vitro experiments, the same preparations of structurally well-defined ligands (LA O55 and LA I–IV) were used.

Kaszowska M., Wojcik M., Siednienko J., Lugowski C, & Lukasiewicz J. (2017). Structure–Activity Relationship of Plesiomonas shigelloides Lipid A to the Production of TNF-α, IL-1β, and IL-6 by Human and Murine Macrophages. Frontiers in Immunology, 8, 1741.

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Cytokine Profiling in Lung Tissues

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The contents of IL-6, TNF-α, IFN-γ, MCP-1, IL-1β, and IL-18 were determined by ELISA method. The lung tissues were crushed with balls at 0–4°C and centrifuged at 12,000 rpm for 20 min. Protein concentration was determined by BCA protein assay kit (Thermo Fisher Scientific, MA, United States). The levels of IL-6, TNF-α, IFN-γ, MCP-1, IL-1β, and IL-18 in lung tissues were measured by using commercially procured ELISA assay kits, including IL-6 ELISA kits (Biolegend, San Diego, United States, Item No. 437107), TNF-α ELISA kits (Biolegend, San Diego, United States, Item No. 438207), IFN-γ ELISA kits (Biolegend, San Diego, United States, Item No. 439007), MCP-1/CCL2 ELISA kits (Genie, London, United Kingdom, Item No. RTFI00038), IL-1β ELISA kits (Genie, London, United Kingdom, Item No. RTDL00552), and IL-18 ELISA kits (Genie, London, United Kingdom, Item No. RTDL00548).

Zhang C., Wang X., Wang C., He C., Ma Q., Li J., Wang W., Xu Y.T, & Wang T. (2021). Qingwenzhike Prescription Alleviates Acute Lung Injury Induced by LPS via Inhibiting TLR4/NF-kB Pathway and NLRP3 Inflammasome Activation. Frontiers in Pharmacology, 12, 790072.

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Quantification of Inflammatory Cytokines

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The amount of TNF-α and IL-6 was quantified in culture supernatants from BMDMs treated as indicated using mouse TNF-α ELISA and IL-6 ELISA kits (BioLegend, CA, USA) following the manufacturer’s instructions. Streptavidin HRP was used to detect bound antibodies, and TMB (Sigma Aldrich) was used as a substrate. The reaction was stopped with 1 M H₂SO₄. Absorbance at 450 and 620 nm was measured on a microplate reader (Thermo Fisher Scientific).

Boonmee A., Benjaskulluecha S., Kueanjinda P., Wongprom B., Pattarakankul T, & Palaga T. (2021). The chemotherapeutic drug carboplatin affects macrophage responses to LPS and LPS tolerance via epigenetic modifications. Scientific Reports, 11, 21574.

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Phytochemical and Bioactivity Analysis

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HRESIMS data were acquired using a Thermo Scientific LTQ-Orbitrap XL instrument (Bremen, Germany). The IR spectra data were recorded using a Shimadzu FTIR-8400S spectrometer (Kyoto, Japan). The UV spectra data were recorded using a Shimadzu UV-2550 spectrophotometer (Kyoto, Japan). The optical rotation data were determined at 25 °C in CH₃OH using a Perkin-Elmer 341 digital polarimeter (Waltham, MA, USA). NMR spectra data were recorded on a Bruker AV III 600 NMR spectrometer (Rheinstetten, Germany). Column chromatography was performed using Sephadex LH-20 (Pharmacia Biotech, Stockholm, Sweden) and silica gel (200–300 mesh). Semipreparative HPLC was conducted on a Chuangxintongheng K1001 analytic LC instrument with an Agilent-SB-Phenyl (250 × 10 mm, 5 μm) and a YMC-C18 column (250 × 10 mm, 5 μm). Penicillin, streptomycin, trypsin, MTT, DMEM, and FBS were purchased from Gibco (Carlsbad, CA, USA). DMSO, L-NAME, and LPS were purchased from Sigma-Aldrich (St. Louis, MO, USA). TNF-α, IL-1β, and IL-6 ELISA kits were purchased from Biolegend (San Diego, CA, USA).

Wang J., Zheng Q., Shi M., Wang H., Fan C., Wang G., Zhao Y, & Si J. (2023). Isolation, Identification, Anti-Inflammatory, and In Silico Analysis of New Lignans from the Resin of Ferula sinkiangensis. Pharmaceuticals, 16(10), 1351.

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Hepatoprotective Effects of Gas6 in Liver Injury

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LPS, D-galactosamine were purchased from Sigma-Aldrich Co (St. Louis, MO, USA). AST, ALT, MPO kits were purchased from the Institute of Jiancheng Bioengineering (Nanjing, China). TNF-α, IL-1β, and IL-6 ELISA kits were purchased from Biolegend (San Diego, CA, USA). Gas6 was purchased from R&D Systems (Minneapolis, MN, USA). Antibodies used for western blot were as follows: anti-Bcl-2 (Proteintech, Rosemont, IL, USA), anti-Bax (Proteintech, Rosemont, IL, USA), anti-ß-actin (Proteintech, Rosemont, IL, USA), NF-κB signal pathway kit (Cell Signaling Technology, Beverly, MA, USA).

Wang Q., Zhao Y, & Zang B. (2020). Anti-inflammation and anti-apoptosis effects of growth arrest-specific protein 6 in acute liver injury induced by LPS/D-GalN in mice. Acta Cirúrgica Brasileira, 35(2), e202000204.

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