β actin c4 sc 477778

RPMI-1640 cell culture medium, FBS (fetal bovine serum), Penicillin/streptomycin (P/S), and trypan blue were purchased from Gibco BRL, CA, USA. Dimethyl sulfoxide (DMSO), Propidium iodide (PI), Ribonuclease A (RNase A), Bovine serum albumin (BSA), Ammonium persulfate (APS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), Cellytic ™ cell lysis reagent, Triton X-100 were from Sigma–Aldrich Chemical company, St. Louis, USA. N–N–N’-N’-Tetramethylene-diamine (TEMED), Polyoxyethylenesorbitan monolaurate (Tween-20), trypran-EDTA, 40% acrylamide/BIS-ACRYLAMIDE (29:1 Ratio), 10X Sodium dodecylsulfate (SDS) buffer, Tris (tris(hydroxymethyl)-aminomethane), 10X SDS-PAGE running buffer were from Amersco company, USA. Apoptosis detection kit (PI/Annexin V-FITC) was from Becton Dickinson, USA. Western lightning chemiluminescene reagent plus (ECL) kit was from NEN Life Science, USA. Anti-cyclin A (ab7956), anticyclin B1 (ab2949), anti-cyclin D1 (ab7958), anticyclin E (ab7959), anti-CDk1/Cdc2 (ab6537), anti-Cdk2 (ab7954), anti-Cdk4 (ab7955), Anti-Cdk6 (ab41870), Anti-p27K1P1 (ab47590), Anti-p21 (ab47452), goat anti-mouse IgG (HRP) horseradish peroxide conjugated antibody were from Abcam company, Cambridge, UK. β-actin (C4): sc-477778, were from Santa Cruz biotechnology, CA, USA.

RPMI cell culture medium, FBS (fetal bovine serum), P/S (penicillin-streptomycin), and trypan blue were purchased from Gibco BRL USA. DMSO (dimethyl sulfoxide), PI (propidium iodide), RNase A (ribonuclease A), BSA (bovine serum albumin), APS (ammonium persulfate), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide), Cellytic ™ M cell lysis reagent, Triton X-100 were from Sigma-Aldrich Chemical company, USA. TEMED (N–N–N′-N′-Tetramethylenediamine), Tween-20 (polyoxyethylenesorbitan monolaurate), trypran-EDTA, 40% acrylamide/BIS-ACRYLAMIDE (29:1 Ratio), 10X SDS (sodium dodecylsulfate) buffer, Tris (tris(hydroxymethy)-aminomethane), 10X SDS-PAGE running buffer (TG-SDS buffer) were from Amersco company, USA. Apoptosis detection kit (PI/Annexin V-FITC) was from Becton Dickinson, USA. Western lightning chemiluminescene reagent plus (ECL) kit was from NEN Life Science, USA. Anti-cyclin A (ab7956), anticyclin E (ab7959), anti-CDk1/Cdc2 (ab6537), anti-Cdk2 (ab7954), Anti-p27K1P1 (ab47590), Anti-p21 (ab47452), goat anti-mouse IgG (HRP) horseradish peroxide conjugated antibody were from Abcam company. β-Actin (C4): sc-477778, caspase-3 (H-277): sc-7418, caspase-8 p20 (H-134):sc-7890, were from Santa Cruz biotechnology.