Bond 3 ihc stainer

Manufactured by Leica

Sourced in Germany

The Bond-III IHC stainer is a fully automated immunohistochemistry (IHC) staining system designed for research and clinical applications. It is capable of performing a range of IHC staining protocols, including basic and advanced staining techniques. The Bond-III IHC stainer provides consistent and reliable results, with the ability to process multiple samples simultaneously.

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Lab products found in correlation

C dpvb 500 hrp, by Medac (2 mentions) Aperio imagescope software, by Leica (1 mentions) Autostainer 480 s, by Medac (1 mentions)

Close spelling variants of this product

Bond III automated IHC/ISH stainer Bond-III automated IHC stainer BOND-III stainer Bond-III IHC Bond III Leica stainer

3 protocols using bond 3 ihc stainer

Quantitative Immunohistochemistry of pSTAT3 in Liver

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Immunohistochemical (IHC) analysis was performed on an automated immunostainer (Leica Bond III IHC Stainer, San Diego, CA). Tissue sections (3 μm) were deparaffinized and underwent heat-induced antigen retrieval using the Tris-EDTA buffer for 20 min. Phospho-STAT3 on Tyr705 (pSTAT3) antibody (Cell Signaling #9145) was used at 1:100 dilution. Digital images were captured at 20x magnification using a whole slide scanner (Leica Aperio ImageScope software) and saved in SVS. format (Aperio). Portal triads and lobular inflammatory foci were annotated as non-hepatocyte areas. Liver lobular areas excluding inflammatory foci were annotated as hepatocyte areas. Quantification of pSTAT3 (pSTAT3 score) was developed in a CLIA laboratory, using nuclear V.9 algorithm (Aperio) and the product of the staining intensity multiplied by the percentage of nuclei ([3 × % of 3+ cells] + [2 × % of 2+ cells] + [1 × % of 1+ cells]).

Jiao J., Sanchez J.I., Saldarriaga O.A., Solis L.M., Tweardy D.J., Maru D.M., Stevenson H.L, & Beretta L. (2022). Spatial molecular and cellular determinants of STAT3 activation in liver fibrosis progression in non-alcoholic fatty liver disease. JHEP Reports, 5(2), 100628.

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Immunohistochemical Analysis of MMR Proteins

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Immunohistochemical (IHC) staining of Formalin-fixed, Paraffin-embedded (FFPE) tissue samples was performed following established routine procedures on a fully automated Bond-III IHC stainer (Leica, Wetzlar, Germany) according to the manufacturer's protocol with the following primary antibodies: MLH1, MSH2, MSH6, PMS2 (all antibodies were purchased from Leica). The level of protein staining in tumor cells was compared to that in normal tissue. MMR protein level was considered deficient if the nuclei showed no, or only very weak immunostaining relative to normal tissue.
IHC of MSH3 was performed on 2-3 μm FFPE tissue specimens using an automated staining system (Medac 480 S Autostainer; Medac, Wedel, Germany). For antigen retrieval a Pre-Treatment (PT)-module (Medac) was used. A rabbit polyclonal antibody for MSH3, raised against an NH2-terminal polypeptide comprising amino acids 1–200, was used at a dilution of 1:100.^{44 (link); 45 (link)} The reaction was developed with horseradish peroxidase (HRP)-conjugated detection system (C-DPVB 500 HRP, Medac) and the 3,3’-Diaminobenzidine (DAB) system (495192F, Medac).

Adam R., Spier I., Zhao B., Kloth M., Marquez J., Hinrichsen I., Kirfel J., Tafazzoli A., Horpaopan S., Uhlhaas S., Stienen D., Friedrichs N., Altmüller J., Laner A., Holzapfel S., Peters S., Kayser K., Thiele H., Holinski-Feder E., Marra G., Kristiansen G., Nöthen M.M., Büttner R., Möslein G., Betz R.C., Brieger A., Lifton R.P, & Aretz S. (2016). Exome Sequencing Identifies Biallelic MSH3 Germline Mutations as a Recessive Subtype of Colorectal Adenomatous Polyposis. American Journal of Human Genetics, 99(2), 337-351.

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Immunohistochemical Analysis of MMR Proteins

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Immunohistochemical (IHC) staining of formalin-fixed, paraffinembedded (FFPE) tissue samples was performed according to established routine procedures on a fully automated Bond-III IHC stainer (Leica) according to the manufacturer's protocol with the following primary antibodies: MLH1, MSH2, MSH6, and PMS2 (all purchased from Leica). The amount of protein staining in tumor cells was compared to that in normal tissue. The amount of MMR protein was considered deficient if the nuclei showed no or only very weak immunostaining in relation to normal tissue.
IHC of MSH3 was performed on 2-3 mm FFPE tissue specimens with an automated staining system (480 S Autostainer, Medac). For antigen retrieval, a pre-treatment module (Medac) was used. A rabbit polyclonal antibody for MSH3, raised against an NH2-terminal polypeptide comprising amino acids 1-200, was used at a dilution of 1:100. 44, 45 The reaction was developed with a horseradish-peroxidase (HRP)-conjugated detection system (C-DPVB 500 HRP, Medac) and the 3,3 0 -diaminobenzidine system (495192F, Medac).

Adam R., Spier I., Zhao B., Kloth M., Marquez J., Hinrichsen I., Kirfel J., Tafazzoli A., Horpaopan S., Uhlhaas S., Stienen D., Friedrichs N., Altmüller J., Laner A., Holzapfel S., Peters S., Kayser K., Thiele H., Holinski-Feder E., Marra G., Kristiansen G., Nöthen M., Büttner R., Möslein G., Betz R., Brieger A., Lifton R., & Aretz S. (2016). Exome Sequencing Identifies Biallelic MSH3 Germline Mutations as a Recessive Subtype of Colorectal Adenomatous Polyposis. The American Journal of Human Genetics, 99(2), 337-351.

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