Example 22
Several milligrams of S-12 were suspended in water. The mean particle size and distributions were determined using a Coulter Multisizer IV (Beckman Coulter, Inc., Brea, Calif.). The distribution shown in
Coulter multisizer 4
Manufactured by Beckman Coulter
Sourced in United States
The Coulter Multisizer IV is a particle size analyzer that uses the Coulter Principle to measure the size and count of particles suspended in a conductive liquid. It provides accurate and precise measurements of particle size distributions.
Automatically generated - may contain errors
7 protocols using coulter multisizer 4
1
Particle Size Analysis of S-12 Suspension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2
Particle Size Analysis of Surface Treated Microparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 22
Measurement of Average Size and Size Distribution of Surface Treated Microparticles (STMP)
Several milligrams of S-12 were suspended in water. The mean particle size and distributions were determined using a Coulter Multisizer IV (Beckman Coulter, Inc., Brea, Calif.). The distribution shown in
3
Particle Size Analysis of S-12 Suspension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 22
Several milligrams of S-12 were suspended in water. The mean particle size and distributions were determined using a Coulter Multisizer IV (Beckman Coulter, Inc., Brea, Calif.). The distribution shown in
4
Particle Size Analysis of S-12 Suspension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 22
Several milligrams of S-12 were suspended in water. The mean particle size and distributions were determined using a Coulter Multisizer IV (Beckman Coulter, Inc., Brea, Calif.). The distribution shown in
5
Measuring Median Volumes of VMs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The median volumes of 4,000–7,000 VMs were measured with a Coulter Multisizer 4 (Beckman Coulter, Brea, CA).[10 (link)]
6
Characterization of C. reinhardtii Mutants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Growth of the C. reinhardtii CrPrp19 RNAi, Maa7, overexpression lines, and wild-type CC124 were determined by a Coulter Multisizer 4 (Beckman Coulter, Fullerton, CA, United States) daily. Cell sizes were measured using a microscope after synchronization culture referred elsewhere (Dillard et al., 2011 (link)). Moreover, cell responses to stresses such as -N, -S, and high salinity (8 g/L sodium acetate) were observed using the plated method.
7
Quantifying Microalgal Lipid Content
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CrCUL RNAi transgenic strains and negative controls (pMaa7IR/X transgenic strains, shown as Maa7 lines) were grown under normal conditions (HSM medium) as described above for 10 days. The cell density was measured using a Coulter™ Multisizer 4 (Beckman Coulter, Fullerton, CA, USA) every day.
For neutral lipid content (NL) examination, cells were stained by 0.5 µg/mL of Nile red (Sigma-Aldrich, Saint Louis, MO, USA) dissolved in 25% (v/v) of DMSO solution. The fluorescence intensity (FI) was measured with a Glomax-Multi Detection System (Promega, Madison, WI, USA) with excitation and emission wavelengths of 530 nm and 575 nm, respectively. Triolein (Sigma-Aldrich, Saint Louis, MO, USA) was used to make a standard curve. The neutral lipid content (NL) was calculated according to the formula:
The lipid accumulation in transgenic strains were observed by a Nikon 80 i fluorescence microscope (Nikon, Kyoto, Japan) after being stained with Nile red. The excitation and emission wavelengths of 480 nm and 580 nm were applied, respectively.
For neutral lipid content (NL) examination, cells were stained by 0.5 µg/mL of Nile red (Sigma-Aldrich, Saint Louis, MO, USA) dissolved in 25% (v/v) of DMSO solution. The fluorescence intensity (FI) was measured with a Glomax-Multi Detection System (Promega, Madison, WI, USA) with excitation and emission wavelengths of 530 nm and 575 nm, respectively. Triolein (Sigma-Aldrich, Saint Louis, MO, USA) was used to make a standard curve. The neutral lipid content (NL) was calculated according to the formula:
The lipid accumulation in transgenic strains were observed by a Nikon 80 i fluorescence microscope (Nikon, Kyoto, Japan) after being stained with Nile red. The excitation and emission wavelengths of 480 nm and 580 nm were applied, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!