GFRs were measured by FITC-labeled sinistrin clearance obtaining seven consecutive blood draws over the time course of 75 min after retro-orbital 1% FITC sinistrin injection (56.1 μg sinistrin/g body weight; Fresenius Kabi). Plasma clearance of FITC-sinistrin was measured fluorometrically (λex = 488 nm/λex = 538 nm) from blood samples obtained via the saphenous vein at seven postinjection time points from 3 to 75 min. Systolic BP measurements were obtained by tail-cuff plethysmography (Kent Scientific). Mice were trained in the system for 3 consecutive days during the same time of day before BP measurements were recorded.
Tail cuff plethysmography
Manufactured by Kent Scientific
Sourced in United States, United Kingdom
Tail-cuff plethysmography is a non-invasive technique used to measure blood pressure in small animals. It utilizes a cuff placed around the animal's tail to detect changes in blood volume, which are then converted into blood pressure measurements.
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Lab products found in correlation
12 protocols using tail cuff plethysmography
1
FITC-Sinistrin Clearance Assay for GFR
2
Measurement of Blood Pressure and Urinary Biomarkers in Rats
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Non-invasive blood pressure was measured on several occasions in conscious rats restrained in a chamber with a controlled temperature between 32 and 34°C. Blood pressure was measured using a tail-cuff plethysmography (Kent Scientific Corporation, Torrington, CT, USA). Urine was sampled during metabolic cage observations for 24 h at the end of the study. Blood was sampled via cardiac catheterization before the animals were terminated. Creatinine was analyzed enzymatically. Albumin was analyzed immunologically. Both performed at laboratory services, St. Olav's Hospital.
3
Noninvasive Blood Pressure Monitoring
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Systolic blood pressure was monitored by noninvasive tail‐cuff plethysmography (Kent Scientific Corp, Torrington, CT) 2 days before the periarterial application of CaCl2 or NaCl (day 0) and weekly for 3 weeks, as detailed in Data S1 .
4
Noninvasive Blood Pressure Monitoring
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Blood pressure was monitored by non-invasive tail-cuff plethysmography (Kent Scientific Corporation, Torrington, USA). Blood pressure assessment began 3 days before minipump implantation in order to habituate the mice to the procedure and continued at three different intervals (days 0, 4, and 7) during 1 week. Mice were placed on a heating platform for 10 min before assessment of blood pressure. A minimum of five measurements were taken until the blood pressure stabilized and, following stabilization, a minimum of eight measurements were taken per mouse and averaged for analysis. Blood pressure was monitored by the same person at the same time of the day.
5
Tail-Cuff Plethysmography for Systolic BP
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Systolic blood pressure was monitored by tail‐cuff plethysmography (Kent Scientific Corp., USA) as detailed in Data S1 . Animals were habituated to the procedure 3 days before blood pressure assessment. The measures were taken 24 hours before CBF analysis.
6
Indirect Blood Pressure Measurement in Mice
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Indirect systolic blood pressure (SBP) and heart rate (HR) were determined by tail-cuff plethysmography (Kent Scientific, Litchfield, CT, USA) in WT or THOP1−/− mice that were fed an SD or a HD. The mice were familiarized with the apparatus for a total of seven days before the measurements were taken. The measurements of SBP and HR were obtained in the 20th and 24th week of experimentation with different diets [73 (link)].
7
Tail-cuff Plethysmography for Murine BP
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SBP was monitored in awake mice using tail-cuff plethysmography (Kent Scientific Corp, Torrington, CT, USA). Mice were warmed on a heating pad preheated at 37 °C for ten minutes before and during blood pressure recordings. Animals were habituated to the procedure three days before blood pressure assessment. Right before the implantation of osmotic minipumps (day 0) and weekly until the NVC analysis, ten blood pressure assessments per mouse were measured and averaged. Blood pressure was monitored by the same person at the same period of the day.
8
Tail-cuff Plethysmography for Systolic BP
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Systolic blood pressure was measured by tail-cuff plethysmography (Kent Scientific, Torrington, UK) as previously described (5 (link), 6 (link), 30 (link)).
9
Tail-Cuff Plethysmography for Blood Pressure
10
Chronic L-NAME Hypertension Model
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