Formalin was purchased from BDH, Poole, England. Duopharma (M) Sdn Bhd, Malaysia, produced the naloxone hydrochloride, morphine sulfate, atropine sulfate used in the study. Nifedipine and diclofenac sodium were procured from Denk Pharma, Germany, whereas glibenclamide was manufactured by Sanofi-Aventis, Guildford, United Kingdom. We obtained yohimbine hydrochloride from Walter Ritter GmbH + Co. KG, Germany whiles prostaglandin E2, bradykinin acetate salt, murine recombinant tumor necrosis factor-alpha (TNF-α), and interleukin-1β, NG-L-nitro-arginine methyl ester (L-NAME) were purchased from the Sigma-Aldrich Inc. St. Louis, MO, United States.
Bradykinin acetate salt
Manufactured by Merck Group
Sourced in Germany, Poland
Bradykinin acetate salt is a laboratory reagent used for research purposes. It is a chemical compound that is utilized in various experimental settings to study biological processes. The primary function of bradykinin acetate salt is to serve as a tool for scientific investigation, without any interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Ng nitro l arginine methyl ester l name, by Merck Group (2 mentions)
Indomethacin, by Merck Group (2 mentions)
Sodium nitroprusside, by Merck Group (2 mentions)
Glibenclamide, by Sanofi (1 mentions)
Formalin, by BD (1 mentions)
Interleukin 1β, by Merck Group (1 mentions)
Acetylcholine, by Merck Group (1 mentions)
Capsaicin, by Merck Group (1 mentions)
Thapsigargin, by Merck Group (1 mentions)
Gsk1016790a, by Merck Group (1 mentions)
Verapamil hydrochloride, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate, by Santa Cruz Biotechnology (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Ubiquitin from bovine erythrocytes, by Merck Group (1 mentions)
Doxycycline, by Thermo Fisher Scientific (1 mentions)
Oxotremorine m, by Merck Group (1 mentions)
Isa 2011b, by MedChemExpress (1 mentions)
Unc3230, by Bio-Techne (1 mentions)
Carbachol, by Merck Group (1 mentions)
7 protocols using bradykinin acetate salt
1
Reagents and Chemicals Used in Study
2
Vasodilation Assay: Coronary Artery Pharmacology
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Acetylcholine (ACh) chloride, sodium nitroprusside (SNP), bradykinin acetate salt, indomethacin, NG-nitro-L-arginine methyl ester (L-NAME) and potassium chloride (KCl) (Chempur, Poland) were obtained from Sigma-Aldrich unless stated otherwise. Stock solutions (10 mM) of indomethacin and L-NAME were prepared in DMSO. These solutions were stored at −20 °C, and appropriate dilutions were made in Krebs-Henseleit solution (KHS in mM: NaCl 120, KCl 4.76, NaHCO3 25, NaH2PO4·H2O 1.18, CaCl2 1.25, MgSO4·7H2O 1.18, glucose 5.5) on the day of the experiment. At these concentrations, DMSO did not alter the reactivity of coronary arteries.
3
Agonist and Inhibitor Characterization of Ion Channels
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Extracellular solution contained (in mM): 145 NaCl, 10 CsCl, 3 KCl, 2 CaCl2, 2 MgCl2, 10 HEPES, 10 D-glucose. Extracellular solution with an elevated concentration of potassium was obtained by replacing 75 mM NaCl with an equimolar amount of KCl. Solutions were adjusted to pH 7.2 with NaOH. Pregnenolone sulfate sodium salt (PS; TRPM3 agonist) and phorbol-12-myristate-13-acetate (PMA; PKC activator) were from Santa Cruz (Heidelberg, Germany). The following substances were purchased from Biomol (Hamburg, Germany): supercinnamaldehyde (SCA; TRPA1 agonist), Gö6983 (GÖ; PKC inhibitor), KN-62 (KN62; CaMKII inhibitor), R-59-022 (R59; DAG kinase inhibitor), RHC-80267 (RHC; DAG lipase inhibitor), exo1 (EXO), and endosidin 2 (END; both inhibitors of exocytosis). The following substances were obtained from Sigma-Aldrich: bradykinin acetate salt (BK; BKR agonist), capsaicin (CAP; TRPV1 agonist), GSK1016790A (GSK; TRPV4 agonist), verapamil hydrochloride (VERA; CaV blocker). Thapsigargin (TH; SERCA inhibitor) was from Sigma and Santa Cruz. AMG9810 (AMG; TRPV1 inhibitor) was obtained from Santa Cruz and Biomol. All chemical reagents were prepared as stock solutions in DMSO, with the exception of BK that was dissolved in acetic acid. The desired concentration of each substance was achieved by diluting stock solution into extracellular perfusing solution on the day of each experiment.
4
Optimizing Mass Spectrometry Experiments
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Ubiquitin from bovine erythrocytes
(≥98% purity) and ammonium acetate were purchased from Sigma
Chemical Co. (St. Louis, MO, USA). Acetic acid and HPLC-grade water
were obtained from Fisher Scientific (Leics, UK). All reagents were
used without further purification. Two 10 μM solutions were
prepared by dissolving ubiquitin in either aqueous 1% Acetic acid
or in 100 mM ammonium acetate aqueous solution, corresponding to the
partially unfolded (charge states 7+ and 8+) and the native protein
conformations (charge states 5+ and 6+), respectively. Papaverine
hydrocloride (99%) was purchased from ACROS Organics and a 0.1 mM
stock solution was prepared in ACN/H2O/AcOH 90:10:0.1 (v/v/v).
A series of dilutions were performed to final concentrations of 0.003,
0.03, 0.15, and 0.3 μM in order to map the stability diagram
of the Omnitrap under different space charge loads. Bradykinin acetate
salt (≥98%) was purchased from Sigma-Aldrich, and 1.2 μM
solution was prepared in H2O/MeOH/AcOH 60:39:1 (v/v/v)
in order to measure the secular frequency of the ions under variable
space charge loads and across a wide range of excitation amplitudes.
Oxidized insulin chain B from bovine pancreas (≥80%) and myoglobin
from equine heart were purchased from Sigma-Aldrich. Insulin chain
B 10 μM solution was prepared in ACN/H2O/AcOH 50:49.9:0.1
(v/v/v) and 10 μM myoglobin solution was prepared in H2O/MeOH/AcOH 49:49:2 (v/v/v).
(≥98% purity) and ammonium acetate were purchased from Sigma
Chemical Co. (St. Louis, MO, USA). Acetic acid and HPLC-grade water
were obtained from Fisher Scientific (Leics, UK). All reagents were
used without further purification. Two 10 μM solutions were
prepared by dissolving ubiquitin in either aqueous 1% Acetic acid
or in 100 mM ammonium acetate aqueous solution, corresponding to the
partially unfolded (charge states 7+ and 8+) and the native protein
conformations (charge states 5+ and 6+), respectively. Papaverine
hydrocloride (99%) was purchased from ACROS Organics and a 0.1 mM
stock solution was prepared in ACN/H2O/AcOH 90:10:0.1 (v/v/v).
A series of dilutions were performed to final concentrations of 0.003,
0.03, 0.15, and 0.3 μM in order to map the stability diagram
of the Omnitrap under different space charge loads. Bradykinin acetate
salt (≥98%) was purchased from Sigma-Aldrich, and 1.2 μM
solution was prepared in H2O/MeOH/AcOH 60:39:1 (v/v/v)
in order to measure the secular frequency of the ions under variable
space charge loads and across a wide range of excitation amplitudes.
Oxidized insulin chain B from bovine pancreas (≥80%) and myoglobin
from equine heart were purchased from Sigma-Aldrich. Insulin chain
B 10 μM solution was prepared in ACN/H2O/AcOH 50:49.9:0.1
(v/v/v) and 10 μM myoglobin solution was prepared in H2O/MeOH/AcOH 49:49:2 (v/v/v).
5
Modeling Parkinson's Disease with α-Synuclein Fibrils
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Doxycycline (J60579; Alfa Aesar) was dissolved in diH2O and SH-SY5Y cells were treated at 3 μg/mL for 72 h. UNC-3230 (5271; Tocris) and ISA-2011B (HY-16937; MedChemExpress) were dissolved in DMSO and cells were treated at 100 nM for 24 h prior to transient transfection or 24 h prior to fixation. Bradykinin acetate salt (B3259; Sigma-Aldrich) was dissolved in diH2O and perfused at 100 μM in 2 mM Ca2+ Ringer’s Solution. Oxotremorine M (O100; Sigma-Aldrich) was dissolved in diH2O and perfused at 100 μM in 2 mM Ca2+ Ringer’s Solution. UTP trisodium salt (U6625; Sigma-Aldrich) was dissolved in diH2O and perfused at 100 μM in 2 mM Ca2+ Ringer’s Solution. Active type 1 recombinant human α-Syn pre-formed fibrils (SPR-322; StressMarq Biosciences) were dissolved in PBS and sonicated for 10 min prior to treatment at 4 μg/mL for 72 h– 14 days. Irrespective of length of α-Syn fibril treatment, all isolated neuronal cultures spent a total of 14 days in culture from initial addition of α-Syn fibrils. α-Syn fibrils were confirmed by immunofluorescence following Triton X- permeabilization and Thioflavin S staining.23 (link)
6
Characterization of PERK Inhibitor Effects
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PERK inhibitor GSK2606414 was a kind gift from Jeffery M. Axten and Rakesh Kumar, GlaxoSmithKline, Collegeville, PA. (S)-3,5-Dihydroxyphenylglycine (DHPG) and thapsigargin were purchased from Tocris, bradykinin acetate salt was purchased from Sigma, carbachol was purchased from EMD Millipore, and Bt3-Ins(1, 4, 5)P3/AM (IP3-AM) was purchased from SiChem.
7
Vascular Pharmacology Reagent Protocol
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Urethane, norepinephrine bitartrate (NE), phenylephrine hydrochloride, sodium nitroprusside, acetylcholine iodide, bradykinin acetate salt, nifedipine, indomethacin, serotonin (5-HT), forskolin, ethylenediaminetetraacetic acid (EDTA), dimethyl sulfoxide (DMSO), KCl, and CaCl 2 .2H 2 O were purchased from Sigma-Aldrich, [1H- [1, 2, 4]
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