Tumors were excised from euthanized animals at indicated times and frozen in a slurry of dry ice and 70% ethanol. Frozen tumors were homogenized on dry ice and then submitted to three freeze-thawcycles. Protein concentrations were measured by bicinchoninic acid (BCA) assay (Thermo Scientific, Hampton, NH). Additionally, prior to confirmatory euthanasia, blood was collected via cardiac puncture. Blood was collected using a syringe bearing a 22-gauge needle (BD, Franklin Lakes, NJ) and transferred to microcentrifuge tubes (Fisher Scientific, Hampton, NH). IL-6 ELISA of serum and tumor samples was performed per manufacturer’s instruction (R&D Systems, Minneapolis, MN). Serum was run undiluted. Tissue homogenate was run at a concentration of 1.0−1.2 mg/ml at dilutions of 1:1 and 1:2. Final values were adjusted for protein concentration and dilution factors. All samples were run in duplicate.
22 gauge needle
Manufactured by BD
Sourced in United States
The 22-gauge needle is a medical device used for various procedures, such as injections, blood draws, and fluid sampling. It is designed to provide a precise and controlled method of accessing veins, tissues, or other bodily structures. The needle's gauge, which refers to its diameter, is a standard measurement that indicates the thickness of the needle. The 22-gauge needle is a commonly used size that offers a balance between ease of use and patient comfort.
Automatically generated - may contain errors
Lab products found in correlation
1 ml slip tip syringe, by BD (2 mentions)
Rlt buffer, by Qiagen (2 mentions)
Model no es5op 10w, by BD (2 mentions)
Bca assay, by Thermo Fisher Scientific (1 mentions)
Microcentrifuge tube, by Thermo Fisher Scientific (1 mentions)
Rneasy micro kit, by Qiagen (1 mentions)
Matrigel, by Corning (1 mentions)
Ack lysing buffer, by Thermo Fisher Scientific (1 mentions)
4 0 pds 2 sutures, by Johnson & Johnson (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Ar 36l, by Percival (1 mentions)
Leibovitz s l 15 medium, by Merck Group (1 mentions)
14 protocols using 22 gauge needle
1
Tumor Excision and Cytokine Analysis
2
Isolating and Profiling PTECs
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PTECs were harvested from devices by injecting 100 µL of detergent (Abcam luminescent kit, part 8206000) into the injection port using a 1 mL slip-tip syringe (BD, 309659) equipped with a 22-gauge needle (BD, 305142). Cell lysate was collected into 900 µL Trizol and frozen at -80 degrees Celsius until extraction. RNA was isolated using a RNeasy Micro Kit (Qiagen, 74004) and the RNA library was prepared and sequenced as described previously73 (link). Aligned data were read into R and summarized as counts per gene using the Bioconductor TxDb.Hsapiens.UCSC.hg38.knownGene package in concert with the GenomicAlignments package (version 3.4.0).
3
RNA Isolation from Kidney Tubules
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To collect RNA samples from PTEC tubules, the PT-MPS devices were flushed with a volume of 1 mL RLT buffer (Qiagen, #79216) delivered through the abluminal inlet using a 1 mL slip-tip syringe (BD, 309659) equipped with a 22-gauge needle (BD, 305142) and collected at the outlet port. The RNA samples in RLT buffer were stored at −80° C until extraction which was performed as described by Lidberg et. al 19 (link).
4
Subcutaneous Tumor Xenograft Model with CAR T Cell Therapy
5
Murine Tissue Harvesting and Cell Isolation
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Femurs, tibias, spleens, and thymuses were harvested from mice immediately after euthanasia with 3%–5% isoflurane and by cervical dislocation. BM cells were flushed into PBS containing 2% fetal bovine serum using a 22-gauge needle (BD Biosciences) and syringe. Single-cell suspensions from spleens, thymuses, and inguinal lymph nodes were prepared by mincing and gently passing cells through 70 μm cell strainers (CELLTREAT Scientific Products). ACK lysing buffer (Invitrogen) was used to remove red blood cells to isolate mononuclear cells.
6
RNA Extraction from PTEC Tubules
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To collect RNA samples from PTEC tubules, the PT-MPS devices were flushed with a volume of 1 mL RLT buffer (Qiagen, #79216) delivered through the abluminal inlet using a 1 mL slip-tip syringe (BD, #309659) equipped with a 22-gauge needle (BD, #305142) and collected at the outlet port. The RNA samples in RLT buffer were stored at −80 oC until extraction as described by Lidberg et al. 11 (link).
7
Cecal Ligation and Puncture Sepsis Model
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CL&P was performed to induce sepsis in accordance with an established protocol (Cuenca et al., 2010 (link)). 8-wk-old male mice were anesthetized with 3% isoflurane and with O2 flow at 1 L/min. A midline incision was made at the abdomen to exteriorize the cecum. Approximately, one-third of the cecum from its distal end was ligated using 3-0 polydioxanone (PDS II) sutures (Ethicon). The ligated cecum was then punctured once with a 22-gauge needle (BD Biosciences) and a small amount of feces (about 1 mm3) was extruded. After replacing the cecum into the abdominal cavity, the abdominal wall was closed with 4-0 PDS II sutures (Ethicon) followed by a skin closure with medical surgical clips (Braintree Scientific). Sham mice underwent the same surgery except for the ligation and puncture of the cecum. All mice were resuscitated by subcutaneous injection of 1 ml of PBS supplemented with antibiotics (imipenem/cilastatin, 0.5 mg/kg body weight).
8
Discography and Discoblock for Discogenic Low Back Pain
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Discography or discoblock at one intervertebral disc level was performed using a standard posterolateral approach with a 22-gauge needle (Becton Dickinson, Franklin Lakes, NJ, USA) in all 52 patients. For discography, the needle was inserted into the center of the disc under fluoroscopic control. Isovist 240 (range, 0.4-3.2 mL; Schering, Berlin, Germany) was injected into each disc until severe pain was provoked or until contrast medium was seen to leak out of the disc into the spinal canal. For discoblock, 0.75 mL of 0.5% bupivacaine was injected into the disc. We defined the treatment as "effective" if patients indicated a Visual Analogue Scale (VAS) pain score at 2 hours that was less than 50% of their initial VAS score before injection.
When pain was both provoked during the discography and decreased after the discoblock, we confirmed a diagnosis of discogenic LBP.
When pain was both provoked during the discography and decreased after the discoblock, we confirmed a diagnosis of discogenic LBP.
9
Electrospinning Apparatus Design for Precise Fiber Fabrication
10
Electrospinning Apparatus Design for Precise Fiber Fabrication
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The electrospinning apparatus was completely enclosed within a 35 × 36 in. dissipative PVC glove box (Terra Universal, Fullerton, CA). Previous studies from the Gilbert laboratory show that fluctuations in the humidity of the electrospinning environment can drastically affect fiber diameter and surface topography [15 (link), 16 (link)]. Thus, this enclosure was important to maintain the relative humidity of the electrospinning environment during fiber fabrication and prevent variations between fiber replicates. Within the glove box, a syringe pump was affixed above a grounded, spinning aluminum disk (22-cm diameter, 1-cm thick). A 5-mL syringe containing electrospinning solutions affixed to a 22 gauge needle (syringes and needles purchased through Becton-Dickenson, Franklin Lakes, NJ) was placed in the syringe pump and attached to a Gamma High Voltage Power Supply (Model No. ES5OP-10W, State College, PA). A collection distance of 5-cm was used between the needle tip and the collection wheel.
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