H1758

Manufactured by Merck Group

Sourced in United States

H1758 is a laboratory equipment product offered by Merck Group. It serves as a device for performing various laboratory tasks and procedures. The core function of H1758 is to provide a tool for scientific experimentation and analysis, without further interpretation of its intended use.

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Lab products found in correlation

J61036, by Thermo Fisher Scientific (3 mentions) P5493, by Merck Group (2 mentions) 96 well plate, by Eppendorf (1 mentions) I9516, by Merck Group (1 mentions) M2128, by Merck Group (1 mentions) P7000, by Merck Group (1 mentions) S8045, by Merck Group (1 mentions) Lyoquest 85, by Telstar (1 mentions) Sml1767, by Merck Group (1 mentions) Penicillin g, by Merck Group (1 mentions) L4408, by Merck Group (1 mentions) Tris buffer, by Thermo Fisher Scientific (1 mentions)

6 protocols using h1758

MTT Assay for Zoledronic Acid Sensitivity

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The MTT method was applied to test the sensitivity of PDLSCs to ZOL as previously reported with some adjustments^{27 (link)}. Briefly, cells (2 × 10³) were seeded into 96-well plates (Eppendorf, Milan, Italy) with 100 µL culture medium per well. Each drug concentration was tested on 16 wells. After 3 and 6 days of treatment, 10 µL MTT solution (M2128, Sigma, Milan, Italy) (2.5 mg/mL) was added to each well. Plates were incubated at 37°C for 3 h and then the supernatant was removed. Formazan crystals were solubilized by adding 0.08N HCl (H1758, Sigma, Milan, Italy) in isopropanol (I9516, Sigma, Milan, Italy) for 30 min at 37°C. The absorbance was measured at 595 nm using a microplate reader (iMark, Bio-Rad Laboratories, Milan, Italy). Values obtained in the absence of cells were considered as control.

Di Vito A., Chiarella E., Baudi F., Scardamaglia P., Antonelli A., Giudice D., Barni T., Fortunato L, & Giudice A. (2020). Dose-Dependent Effects of Zoledronic Acid on Human Periodontal Ligament Stem Cells: An In Vitro Pilot Study. Cell Transplantation, 29, 0963689720948497.

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Decellularized Endometrial Matrix Preparation

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Isolated DC endometrial tissue stock was flash-frozen in a mortar with liquid N₂, milled manually, and lyophilized (Lyoquest-85, Telstar, Valencia’s Polytechnic University) over 96 h at 20 Pa. The resulting endometrial lyophilized powder was digested and neutralized using a modified protocol (Brown et al., 2017 (link)). Briefly, 1% (w/v) lyophilized powder was suspended in 0.01 M HCl (H1758, Sigma-Aldrich) with 0.1% pepsin (P7000, Sigma-Aldrich) and digested for 48 h under agitation. The solution was left on ice and neutralized with 10% (v/v) 0.1 M NaOH (S8045, Sigma-Aldrich), 11.11% (v/v) 10X PBS (P5493, Sigma-Aldrich), and 1X PBS was used to reach the desire concentration. The resulting EndoECM solution was stored at −80°C.
This process was also performed with isolated DC myometrial and No-DC endometrial tissue stocks to create myometrial extracellular matrix (MyoECM) and No-DC endometrial matrix (No-DC Endo). MyoECM and No-DC Endo were used as controls for subsequent proteomic analyses.

López-Martínez S., Campo H., de Miguel-Gómez L., Faus A., Navarro A.T., Díaz A., Pellicer A., Ferrero H, & Cervelló I. (2021). A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current Human in vitro Models and Future in vivo Applications. Frontiers in Bioengineering and Biotechnology, 9, 639688.

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Nanopore Sensing of DNA using Electrolytes

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All electrolytes were prepared (purchased through Sigma–Aldrich) by dissolving the as-supplied salts LiCl (213 233), NaCl (S5886), KCl (P9333), RbCl (R2252), and CsCl (289 329)) in >18 MΩ cm resistivity ultra-pure water (ARS-102 Aries high purity water systems). All electrolytes were buffered using 10 mM Tris buffer (J61036, Fisher Scientific). Electrolytes were then filtered using 0.22 μm vacuum filter units (Millipore Sigma Stericup, S2GPU05RE) and the pH was adjusted by adding concentrated drops of HCl (H1758, Sigma–Aldrich) and/or KOH (306 568, Sigma–Aldrich). Both pH and conductivity were measured using an Orion Star pH meter.
Double-stranded DNA (dsDNA, 1kb) was purchased from Fisher Scientific (10787 018). The dsDNA was added to the cis side to a final concentration of ~17 ng/μL. All experiments were done using 4 M LiCl buffered using 10 mM tris buffer, +200 mV of applied voltage, 100 kHz low pass filtering, and 250 kHz sampling frequency.

Saharia J., Bandara Y.M., Karawdeniya B.I., Alexandrakis G, & Kim M.J. (2021). Assessment of 1/f noise associated with nanopores fabricated through chemically tuned controlled dielectric breakdown. Electrophoresis, 42(7-8), 899-909.

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Metabolic Activity of IVD Cells and C. acnes Coculture

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To assess the influence of co-culturing IVD cells and C. acnes and of treatment with sparstolonin B (SML1767, Sigma, St. Louis, MO, USA) on metabolic activity, the MTT assay (3-[4–dimethylthiazol-2-yl]μ-2,5-diphenyl tetrazolium bromide formazan, M2003, Merck, Darmstadt, Germany) was used. IVD cells were seeded and co-cultured with C. acnes (MOI 1, 10, and 100) with or without stimulation of different sparstolonin B concentrations (10, 15, 20, 25, 50, and 100 μM). Extracellular bacteria were killed by incubation for 2 h with penicillin G (0.128 mg/mL, 13752, Merck, Darmstadt, Germany). The cells were then incubated with MTT in DMEM/F12 (5 mg/mL) for 2 h at 37 °C, 0.04 M HCl (H1758, Sigma, St. Louis, MO, USA) in isopropanol (2-propanol, I9516, Sigma, St. Louis, MO, USA) was used as a solubilization solution, and the absorbance was measured at 570 nm. Metabolic activity was calculated relative to the untreated control.

Schmid B., Hausmann O., Hitzl W., Achermann Y, & Wuertz-Kozak K. (2020). The Role of Cutibacterium acnes in Intervertebral Disc Inflammation. Biomedicines, 8(7), 186.

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Nanopore Electrolyte Solution Preparation

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All electrolytes including LiCl (L4408, Sigma-Aldrich, USA) and KCl were dissolved in ultra-pure water (ARS-102 Aries high purity water systems) with ~18 MΩ∙cm resistivity. Each solution contains 10 mM of either tris buffer (J61036, Fisher Scientific, USA) or phosphate buffer saline (P5493, Sigma-Aldrich, USA). The former was used for translocation experiments whereas the latter was used to acquire current-voltage (I-V) curves for pore-diameter estimation. The solutions were then filtered using a filtration system with a Polyethersulfone membrane (S2VPU02RE, Fisher Scientific). Caution: dissolving LiCl in water is an exothermic process. After the electrolyte solution reached the room temperature, the pH was adjusted by adding HCl (H1758, Sigma-Aldrich, USA) or KOH (306568, Sigma-Aldrich, USA) dropwise while gently stirring the electrolyte solution continuously. Caution: these are concentrated solutions and should only be open inside a properly functioning fume hood. Both pH and conductivity of the electrolyte solutions were measured and typically, a 2M LiCl solution at pH ~7 would have a conductivity of ~12 S/m whereas a 1 M KCl solution at pH~7 would have a conductivity of ~11 S/m.

Karawdeniya B.I., D. Y. Bandara Y.M., Khan A.I., Chen W.T., Vu H.A., Morshed A., Suh J., Dutta P, & Kim M.J. (2020). Adeno-Associated Virus Characterization for Cargo Discrimination through Nanopore Responsiveness. Nanoscale, 12(46), 23721-23731.

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Lithium Chloride Electrolyte Preparation

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All experiments were carried out with LiCl as the principal electrolyte salt. All electrolytes were prepared by dissolving the as-supplied LiCl in ultra-pure water (>18 MΩ cm) followed by the addition of Tris buffer (J61036, Fisher Scientific) to a final concentration of 10 mM. Solutions were then filtered using 0.22 μm filters (S2GPT05RE, Fisher Scientific). The pH was adjusted by adding concentrated drops of HCl (H1758, Sigma Aldrich) or KOH (306568, Sigma Aldrich) and measured using an Orion Star ™ pH meter.

Saharia J., Bandara Y.M.N.D.Y., Karawdeniya B.I., Dwyer J.R, & Kim M.J. (2023). Over One Million DNA and Protein Events Through Ultra-Stable Chemically-Tuned Solid-State Nanopores. Small (Weinheim an der Bergstrasse, Germany), 19(29).

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